Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,713,568	T→C	W225R (TGG→CGG)	srmB →	ATP‑dependent RNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,713,568	0	T	C	100.0%	198.7 / NA	55	W225R (TGG→CGG)	srmB	ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/31);  total (24/31)

AGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGTGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAA  >  NC_000913/2713443‑2713691                                                                                                                               |                                                                                                                            aGACCCTGCTGTGTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCgcgc                                                                                                                <  2:1055348/139‑1 (MQ=255) aGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCgcgc                                                                                                                <  2:1241131/139‑1 (MQ=255)   aCCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGGAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCg                                                                                                              >  1:907579/1‑139 (MQ=255)   aCCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCg                                                                                                              >  1:111109/1‑139 (MQ=255)   aCCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCg                                                                                                              >  2:1133022/1‑139 (MQ=255)        gCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGCCTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCgatgat                                                                                                         >  2:1114008/1‑139 (MQ=255)                cGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCa                                                                                                 <  2:448648/139‑1 (MQ=255)                      cGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAAc                                                                                           <  2:261011/139‑1 (MQ=255)                        cTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGGAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACcg                                                                                         <  2:907579/139‑1 (MQ=255)                           gAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGt                                                                                      >  1:1008764/1‑139 (MQ=255)                              ggCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGc                                                                                   <  2:725981/139‑1 (MQ=255)                                cgatgcgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCgcgc                                                                                                                <  1:1162181/108‑1 (MQ=255)                                cgatgcgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCgcgc                                                                                                                >  2:1162181/1‑108 (MQ=255)                                cgatgcgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTg                                                                                 >  2:592179/1‑139 (MQ=255)                                 gatgcgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCTCCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTgg                                                                                >  2:165924/1‑139 (MQ=255)                                   tgcgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCg                                                                                                              <  2:738739/107‑1 (MQ=255)                                   tgcgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCg                                                                                                              >  1:738739/1‑107 (MQ=255)                                    gcgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGc                                                                             <  1:1114008/139‑1 (MQ=255)                                     cgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTAAGCATAAAACCGCGTTGCTGGTGCa                                                                            <  2:1369422/139‑1 (MQ=255)                                      gatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCAt                                                                           <  2:463764/139‑1 (MQ=255)                                       atTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATc                                                                          <  1:313057/139‑1 (MQ=255)                                               cTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTaaaa                                                                  <  2:638517/139‑1 (MQ=255)                                                  tgtcGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCgat                                                                                                            >  2:830614/4‑94 (MQ=255)                                                  tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACcgcg                                                                                                                 >  2:475367/1‑89 (MQ=255)                                                  tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACcgcg                                                                                                                 <  1:475367/89‑1 (MQ=255)                                                  tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCgat                                                                                                            <  1:830614/94‑1 (MQ=255)                                                  tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGc                                                                             >  1:113668/1‑125 (MQ=255)                                                  tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGc                                                                             <  2:113668/125‑1 (MQ=255)                                                  tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                            <  1:1125222/126‑1 (MQ=255)                                                  tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                            >  2:1125222/1‑126 (MQ=255)                                                  tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAg                                                               >  1:249780/1‑139 (MQ=255)                                                   gCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCTCCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGc                                                              <  1:591721/139‑1 (MQ=255)                                                       aGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGa                                                          >  2:1124830/1‑139 (MQ=255)                                                           tCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCg                                                      <  1:165924/139‑1 (MQ=255)                                                                tgGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCg                                                 <  2:111109/139‑1 (MQ=255)                                                                       tCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAAt                                           >  1:1178708/1‑139 (MQ=255)                                                                          ggTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGAc                                                    >  2:147979/1‑126 (MQ=255)                                                                          ggTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGAc                                                    <  1:147979/126‑1 (MQ=255)                                                                           gTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTgtgt                                      <  2:1008764/139‑1 (MQ=255)                                                                             ggAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGttt                                    <  1:1124830/139‑1 (MQ=255)                                                                                  tttCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCg                                                                                                              <  2:581802/60‑1 (MQ=255)                                                                                  tttCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCg                                                                                                              >  1:581802/1‑60 (MQ=255)                                                                                   ttCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGt                              <  2:249780/139‑1 (MQ=255)                                                                                         cAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAagcgt                        <  2:1244661/139‑1 (MQ=255)                                                                                           aTCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAAtt                                          <  2:360001/119‑1 (MQ=255)                                                                                           aTCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAAtt                                          >  1:360001/1‑119 (MQ=255)                                                                                            tCCCTCCACCCGTGAGCGCAAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAagcgtga                      <  2:1178708/139‑1 (MQ=255)                                                                                                       gTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGc                                                >  1:148384/1‑101 (MQ=255)                                                                                                       gTGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGc                                                <  2:148384/101‑1 (MQ=255)                                                                                                        tGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                            <  2:529964/72‑1 (MQ=255)                                                                                                        tGAGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                            >  1:529964/1‑72 (MQ=255)                                                                                                          aGCGC‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCt       >  2:103247/1‑139 (MQ=255)                                                                                                              c‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGAc                                                    <  2:238140/90‑1 (MQ=255)                                                                                                              c‑AAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGAc                                                    >  1:238140/1‑90 (MQ=255)                                                                                                                aaaaaaaTTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCaa  >  1:365819/1‑139 (MQ=255)                                                                                                                               |                                                                                                                            AGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGC‑AAAAAAATTCATCAGTGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAA  >  NC_000913/2713443‑2713691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑