| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,370,044 | (T)9→8 | intergenic (+41/‑172) | pspE → / → ycjM | thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,370,036 | 0 | T | . | 96.3% | 205.9 / 2.2 | 54 | intergenic (+33/‑180) | pspE/ycjM | thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base . (25/27); total (26/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
GGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGA > NC_000913/1369909‑1370173 | ggCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGttttttttctgt < 2:332845/139‑5 (MQ=255) caagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTca < 1:411629/139‑1 (MQ=255) aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTcac < 2:773964/139‑1 (MQ=255) caaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACAcc < 1:468522/139‑1 (MQ=255) cTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATc < 1:834659/139‑1 (MQ=255) tAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACa > 2:439705/1‑135 (MQ=255) tAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACa < 1:439705/135‑1 (MQ=255) gCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGTAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTc < 2:971366/119‑1 (MQ=255) gCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGCGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTc > 1:971366/1‑119 (MQ=255) aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCg > 2:240698/1‑139 (MQ=255) cccACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATc > 1:1382278/1‑120 (MQ=255) cccACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATc < 2:1382278/120‑1 (MQ=255) ccACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACAcc < 2:1255484/108‑1 (MQ=255) ccACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACAcc > 1:1255484/1‑108 (MQ=255) aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGc < 2:886352/139‑1 (MQ=255) cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTcaca < 2:524874/103‑1 (MQ=255) cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTcaca > 1:524874/1‑103 (MQ=255) gTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTca > 2:1445475/1‑100 (MQ=255) gTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTca < 1:1445475/100‑1 (MQ=255) gTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTc > 1:1500617/1‑139 (MQ=255) agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTca > 1:1217178/1‑96 (MQ=255) agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTca < 2:1359354/96‑1 (MQ=255) agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTca > 1:1359354/1‑96 (MQ=255) agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTca < 2:1217178/96‑1 (MQ=255) agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACa > 2:542644/1‑109 (MQ=255) agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACa < 1:542644/109‑1 (MQ=255) aaaGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGttttttttctgt > 1:1180437/1‑73 (MQ=255) aaaGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGttttttttctgt < 2:1180437/77‑5 (MQ=255) cATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACAcc < 2:481011/78‑1 (MQ=255) cATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACAcc > 1:481011/1‑78 (MQ=255) cATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTata < 2:6864/87‑3 (MQ=255) cATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTata > 1:6864/1‑85 (MQ=255) aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACa < 2:469703/86‑1 (MQ=255) aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACa > 1:469703/1‑86 (MQ=255) aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaa > 2:443051/1‑139 (MQ=255) tGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTca > 1:697745/1‑66 (MQ=255) tGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTca < 2:697745/66‑1 (MQ=255) aaGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTcac > 2:1185688/1‑62 (MQ=255) aaGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTcac < 1:1185688/62‑1 (MQ=255) cAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt > 2:1376364/1‑94 (MQ=255) cAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt < 1:1376364/94‑1 (MQ=255) gTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTaaaa < 2:1174973/139‑1 (MQ=255) aaaaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTc < 1:798925/47‑1 (MQ=255) aaaaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTc > 2:798925/1‑47 (MQ=255) aaaaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTcc > 1:1251741/1‑104 (MQ=255) aaaaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTcc < 2:1251741/104‑1 (MQ=255) aaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCtt < 2:778192/139‑1 (MQ=255) ttCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt > 2:1061508/1‑139 (MQ=255) ttCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt > 1:1071519/1‑139 (MQ=255) aGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACCTCCCCATAG‑‑‑ATTTGCGTCAGCTCACaaa > 2:739968/1‑73 (MQ=255) aGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAG‑‑‑ATTTGCGTCAGCTCACaaa < 1:739968/73‑1 (MQ=255) cgcTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACa > 2:1124980/1‑40 (MQ=255) cgcTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACa < 1:1124980/40‑1 (MQ=255) gcTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAt > 2:692237/1‑139 (MQ=255) gcTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAt > 1:804146/1‑139 (MQ=255) gcgcCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTg < 1:692237/139‑1 (MQ=255) gTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGa < 1:240698/139‑1 (MQ=255) | GGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGA > NC_000913/1369909‑1370173 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |