| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,803,347 | (T)7→6 | coding (2000/2145 nt) | nrdE → | ribonucleoside‑diphosphate reductase 2, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,803,341 | 0 | T | . | 97.1% | 277.3 / 2.2 | 70 | coding (1994/2145 nt) | nrdE | ribonucleoside‑diphosphate reductase 2, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base . (34/34); total (34/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
TTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGCTTTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAGGCTGC > NC_000913/2803206‑2803474 | tttACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGCtttt > 2:200516/1‑139 (MQ=255) tacCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGCTTTTTTccc < 1:924196/139‑1 (MQ=255) tacCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGCTTTTTTccc < 1:200516/139‑1 (MQ=255) ccTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGa < 2:1399895/139‑1 (MQ=255) gCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATAc < 2:1513956/139‑1 (MQ=255) tttATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGccac > 2:1019139/1‑139 (MQ=255) cTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTcgc < 1:710356/139‑1 (MQ=255) cTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTcgc < 2:361000/139‑1 (MQ=255) cTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGAAACCTACGCGGAAGCGACTCGCCATGTCGATCAGGTGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATcaacaa > 2:1117822/1‑139 (MQ=255) cgcTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTCTTCCCCGATACCGCCACCACTCGCGATATCAACAAAgcg < 2:326472/139‑1 (MQ=255) tATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGAt > 1:486195/1‑139 (MQ=255) aCGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGcc > 1:1277490/1‑139 (MQ=255) aCGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGcc < 2:628732/139‑1 (MQ=255) cGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCt > 1:674220/1‑139 (MQ=255) cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGa < 2:1521983/93‑1 (MQ=255) cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGa > 1:1521983/1‑93 (MQ=255) cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATa < 1:894633/95‑1 (MQ=255) cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCg < 2:68425/98‑1 (MQ=255) cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCg > 1:68425/1‑98 (MQ=255) cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGcc > 2:829063/1‑136 (MQ=255) cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGcc > 1:767211/1‑136 (MQ=255) cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGcc < 1:829063/136‑1 (MQ=255) cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGcc < 2:767211/136‑1 (MQ=255) cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTgg > 1:969923/1‑139 (MQ=255) cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTgg > 1:582147/1‑139 (MQ=255) cTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGACTGTCGCTGACGC‑TTTTTTCCCCGATa > 2:894633/1‑95 (MQ=255) tACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGgcg > 2:310074/1‑139 (MQ=255) aCGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGgcgc > 1:684605/1‑139 (MQ=255) cGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCa < 1:1019139/139‑1 (MQ=255) cgcAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCa > 1:320144/1‑139 (MQ=255) aGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCaaaa < 2:486195/139‑1 (MQ=255) aGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCaaaa < 2:752726/139‑1 (MQ=255) aGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCaaaa < 2:674220/139‑1 (MQ=255) aaaaGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACg < 2:582147/139‑1 (MQ=255) aaaaGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACg < 1:310074/139‑1 (MQ=255) aaaaGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACg < 1:8924/139‑1 (MQ=255) aaaGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCATGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGc < 1:1117822/139‑1 (MQ=255) cGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACa > 1:295905/1‑139 (MQ=255) acacCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGCttttttcc < 1:1468008/59‑3 (MQ=255) acacCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGCttttttcc > 2:1468008/1‑57 (MQ=255) acacCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGcc > 1:1121983/1‑106 (MQ=255) acacCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGcc < 2:1121983/106‑1 (MQ=255) acCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCaa < 2:120081/112‑1 (MQ=255) acCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCaa > 1:120081/1‑112 (MQ=255) ccTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACg < 2:586042/124‑1 (MQ=255) ccTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACg > 1:586042/1‑124 (MQ=255) cgcgGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGc > 1:1127882/1‑139 (MQ=255) cgcgGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGc > 1:210251/1‑139 (MQ=255) aaGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAgcg < 2:1221866/82‑1 (MQ=255) aaGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAgcg > 1:1221866/1‑82 (MQ=255) gCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGAt > 2:504915/1‑139 (MQ=255) cTCGCCATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGgcg < 1:504915/139‑1 (MQ=255) cATGTCGATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGa < 2:1277490/139‑1 (MQ=255) gATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTc > 1:32193/1‑48 (MQ=255) gATCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTc < 2:32193/48‑1 (MQ=255) tCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGa < 2:77091/121‑1 (MQ=255) tCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGa > 1:77091/1‑121 (MQ=255) tCAGGGGCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGAAGGCACTg < 2:210251/139‑1 (MQ=255) gCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACg > 2:1081275/1‑90 (MQ=255) gCTGTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACg < 1:1081275/90‑1 (MQ=255) gCTGTCGCTGACGC‑TTTTTTCCCCGATACCACCACCACTCGCGATATCAACAAAgcgc > 1:1218272/1‑58 (MQ=38) gCTGTCGCTGACGC‑TTTTTTCCCCGATACCACCACCACTCGCGATATCAACAAAgcgc < 2:1218272/58‑1 (MQ=38) gTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAg > 1:156123/1‑139 (MQ=255) gTCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATT‑CATCCGCCTGCGTCAGATGGCGCTTGAAGGGCCTGAAAATGAAgg > 1:557051/1‑139 (MQ=255) tCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGcc > 1:1246450/1‑65 (MQ=255) tCGCTGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGcc < 2:1246450/65‑1 (MQ=255) tGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGcc > 1:1227749/1‑61 (MQ=255) tGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGcc < 2:1227749/61‑1 (MQ=255) tGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCt < 1:1398096/99‑1 (MQ=255) tGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCt > 2:1398096/1‑99 (MQ=255) tGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGa > 2:1489440/1‑117 (MQ=255) tGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGa < 1:1489440/117‑1 (MQ=255) tGACGC‑TTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAGGCTGc > 2:218085/1‑139 (MQ=255) | TTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGCTTTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAGGCTGC > NC_000913/2803206‑2803474 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |