| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 723,528 | (T)8→9 | coding (887/2685 nt) | kdpD ← | fused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 723,520 | 1 | . | T | 94.4% | 161.5 / 2.2 | 54 | coding (895/2685 nt) | kdpD | fused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein |
| Reads supporting (aligned to +/- strand): ref base . (0/3); new base T (27/24); total (27/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
TCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGA > NC_000913/723397‑723645 | tCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTa > 2:471418/1‑139 (MQ=255)tCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTa > 1:1436189/1‑139 (MQ=255) ttGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAg < 1:471418/139‑1 (MQ=255) tGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGc > 2:1454555/1‑139 (MQ=255) gagaTTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTg < 2:1211180/139‑1 (MQ=255) gTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTgcagggcag < 2:1436189/139‑1 (MQ=255) gTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTgcagggcag > 1:789909/1‑139 (MQ=255) cATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTgcagggcagg < 1:1449916/131‑1 (MQ=255) cATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTgcagggcagg > 2:1449916/1‑131 (MQ=255) cATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCaa > 1:1025189/1‑139 (MQ=255) cTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTACGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCACCATACACCGCGTGcc < 2:1025189/139‑1 (MQ=255) tctcTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTg > 1:1134795/1‑104 (MQ=255) tctcTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTg < 2:1134795/104‑1 (MQ=255) tctTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACg < 1:459034/139‑1 (MQ=255) ttCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCt > 2:680829/1‑139 (MQ=255) cGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTAcc > 1:919038/1‑139 (MQ=255) cTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAA‑TGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTgcagggcag > 1:1113478/1‑102 (MQ=255) cTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAA‑TGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTgcagggcag < 2:1113478/102‑1 (MQ=255) gATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGGCGTAAGGCGCTGAGAATTGCCCGACGTTTT‑TTTTTTCCGGTAAGCGGTg < 1:362635/93‑1 (MQ=255) gATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTT‑TTTTTTCCGGTAAGCGGTg > 2:362635/1‑93 (MQ=255) gATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGcc < 1:680829/139‑1 (MQ=255) tgtTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAg < 2:530919/77‑1 (MQ=255) tgtTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAg > 1:530919/1‑77 (MQ=255) tgtTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGtt < 1:751863/95‑1 (MQ=255) tgtTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGtt > 2:751863/1‑95 (MQ=255) cGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGcc > 1:1476349/1‑123 (MQ=255) cGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGcc < 2:1476349/123‑1 (MQ=255) tcCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgccg < 2:1118084/132‑1 (MQ=255) tcCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgccg > 1:1118084/1‑132 (MQ=255) tcCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCt > 2:818747/1‑139 (MQ=255) cgcgCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTgcg < 2:1153560/139‑1 (MQ=255) gcgcCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAAGTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTgcg < 1:979595/139‑1 (MQ=255) cAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAg > 2:931312/1‑59 (MQ=39) cAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑‑TTCTTTTTTCCGGTAAg < 1:931312/59‑1 (MQ=39) ccAGACGTAAGGCGCTGAGAATTGCCCGACGTTTT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCg > 2:1235107/1‑139 (MQ=255) ccAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTg > 1:204894/1‑53 (MQ=255) ccAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTg < 2:1189470/53‑1 (MQ=255) ccAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTg > 1:1189470/1‑53 (MQ=255) ccAGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTg < 2:204894/53‑1 (MQ=255) aGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAg > 2:558179/1‑46 (MQ=39) aGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAg < 1:558179/46‑1 (MQ=39) aGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGTGTGcc > 1:946724/1‑83 (MQ=255) aGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTg > 1:276656/1‑139 (MQ=255) aGACGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTg > 2:1379500/1‑139 (MQ=255) aGACGTAAGGCGCTGAGAATTGCCCGACG‑‑tt‑ttttt > 1:1260018/1‑36 (MQ=255) aGACGTAAGGCGCTGAGAATTGCCCGACG‑‑tt‑ttttt < 2:1260018/36‑1 (MQ=255) aGACGTAAGGCGCTGAGAATTGCCCGACG‑‑TTCTTTTTTCCGTTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGcc < 2:946724/83‑1 (MQ=255) aCGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAg < 1:580927/44‑1 (MQ=38) aCGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAg > 2:580927/1‑44 (MQ=38) aCGTAAGGCGCTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGcc < 2:1463941/139‑1 (MQ=255) cTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCg < 1:760773/130‑1 (MQ=255) cTGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCg > 2:760773/1‑130 (MQ=255) tGAGAATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGc > 1:1519348/1‑139 (MQ=255) gaATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGa < 2:1519348/139‑1 (MQ=255) gaATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGa < 1:1454555/139‑1 (MQ=255) gaATTGCCCGACGT‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGa < 2:919038/139‑1 (MQ=255) | TCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑‑TT‑TTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGA > NC_000913/723397‑723645 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |