| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,920,205 | (T)7→8 | intergenic (+62/‑18) | yebT → / → rsmF | MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,920,198 | 1 | . | T | 97.8% | 144.8 / ‑4.1 | 46 | intergenic (+55/‑25) | yebT/rsmF | MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (22/23); total (22/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCCCG > NC_000913/1920072‑1920330 | ggCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑‑tttttt‑ttgcca > 2:1241806/1‑134 (MQ=255) gCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAg < 2:128094/139‑1 (MQ=255) ccTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTCTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAg < 2:1300775/129‑1 (MQ=255) ccTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTCTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAg > 1:1300775/1‑129 (MQ=255) gTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCg < 2:1250646/139‑1 (MQ=255) ggAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCg < 2:763762/102‑1 (MQ=255) ggAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCg > 1:763762/1‑102 (MQ=255) tGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTAc < 1:992176/90‑1 (MQ=255) tGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGacac > 2:441775/1‑139 (MQ=255) tGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACGGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTAc > 2:992176/1‑90 (MQ=255) cgcTTCCCAAATAATGCCCACTGCTCGGGCGTGCCTGCGCCGGAGTGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGcc < 2:1487314/100‑1 (MQ=255) cgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGcc > 1:1487314/1‑100 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑‑tttttt‑ttg > 2:677093/1‑61 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑‑tttttt‑ttg < 1:677093/63‑3 (MQ=255) cTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑‑TTTTTTGTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCttg < 1:1428428/139‑2 (MQ=255) gCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAacacac > 1:313889/1‑86 (MQ=255) gCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAacacac < 2:313889/86‑1 (MQ=255) tCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACGGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACg < 1:235757/139‑1 (MQ=255) ggCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAaca < 1:1075260/78‑1 (MQ=255) ggCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAaca > 2:1075260/1‑78 (MQ=255) tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCAt > 1:397599/1‑139 (MQ=255) tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCAt > 1:1392985/1‑139 (MQ=255) ccTGCGCCGGAGTGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGt < 1:1028198/50‑1 (MQ=255) ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGt > 2:1028198/1‑50 (MQ=255) ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑‑tttttt‑ttgcc < 2:693579/47‑5 (MQ=255) ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑‑tttttt‑ttgcc > 1:693579/1‑43 (MQ=255) ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑‑tttttt‑ttgcc < 1:359950/47‑5 (MQ=255) ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑‑tttttt‑ttgcc > 2:359950/1‑43 (MQ=255) gcgcCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTtgat < 1:441775/139‑1 (MQ=255) gcCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGAc < 2:186922/86‑1 (MQ=255) gcCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGAc > 1:186922/1‑86 (MQ=255) ggAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAacacac < 1:1374815/67‑1 (MQ=255) ggAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAacacac > 2:1374815/1‑67 (MQ=255) aGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCg < 1:1315738/52‑1 (MQ=255) aGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCg > 2:1315738/1‑52 (MQ=255) aGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTc > 1:817100/1‑139 (MQ=255) gCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTAtt > 1:10367/1‑64 (MQ=255) gCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTAtt < 2:10367/64‑1 (MQ=255) gCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGCGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATg < 1:479217/102‑1 (MQ=255) gCTAAACTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGCGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATg > 2:479217/1‑102 (MQ=255) aaaCTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCg > 2:692569/1‑40 (MQ=38) aaaCTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCg < 1:692569/40‑1 (MQ=255) aaaCTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGAt > 1:871275/1‑98 (MQ=255) aaaCTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGAt < 2:871275/98‑1 (MQ=255) aaCTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCg > 1:1186366/1‑104 (MQ=255) aaCTGCGCGCCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCg < 2:1186366/104‑1 (MQ=255) cgcgcCTGTTTTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGAtt > 1:805409/1‑119 (MQ=255) cgcgcCTGTTTTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGAtt < 2:805409/119‑1 (MQ=255) cgcgcCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAacacac > 1:704660/1‑47 (MQ=39) cgcgcCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAacacac < 2:704660/47‑1 (MQ=39) cgcgcCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGa < 2:1153334/62‑1 (MQ=255) cgcgcCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGa > 1:1153334/1‑62 (MQ=255) cgcCTGT‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCCCg < 2:424054/139‑1 (MQ=255) | GGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑‑TTTTTT‑TGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCCCG > NC_000913/1920072‑1920330 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |