| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,950,559 | (C)5→6 | intergenic (‑37/‑273) | aspS ← / → yecD | aspartyl‑tRNA synthetase/isochorismatase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,950,554 | 1 | . | C | 98.3% | 200.0 / ‑3.5 | 58 | intergenic (‑32/‑278) | aspS/yecD | aspartyl‑tRNA synthetase/isochorismatase family protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (27/30); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
ATGAAGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑C‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTA > NC_000913/1950416‑1950682 | aTGAAGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATg > 1:417363/1‑139 (MQ=255) gCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACATAGCTGCCGGCGGATGCC‑CCCctgctg < 2:991526/139‑1 (MQ=255) ccAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCa > 1:172451/1‑139 (MQ=255) tCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGAc > 1:1464023/1‑139 (MQ=255) cgacgGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTc < 1:908347/139‑1 (MQ=255) gTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGc < 2:1457332/139‑1 (MQ=255) aCCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATAACCCTTAACTTAGCTGCCGGCGGATG‑CACCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGt > 2:289184/1‑139 (MQ=255) cACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑c‑ccccc < 1:523000/92‑2 (MQ=255) cACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑c‑ccccc > 2:523000/1‑91 (MQ=255) aCCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACtga < 2:1464023/139‑1 (MQ=255) tgcCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCa < 1:614448/94‑1 (MQ=255) tgcCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCa > 2:614448/1‑94 (MQ=255) ccccACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAAc > 2:775597/1‑128 (MQ=255) ccccACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAAc < 1:775597/128‑1 (MQ=255) ccccACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGaaaaa > 1:1035512/1‑139 (MQ=255) aCGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCACAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc < 2:172451/139‑1 (MQ=255) cGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCg < 2:676156/100‑1 (MQ=255) cGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCg > 1:676156/1‑100 (MQ=255) gACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCtat > 1:37514/1‑139 (MQ=255) aGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc > 2:736884/1‑124 (MQ=255) aGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc < 1:736884/124‑1 (MQ=255) gCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCg > 2:1042997/1‑85 (MQ=255) gCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCg < 1:1042997/85‑1 (MQ=255) gCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc > 2:819779/1‑123 (MQ=255) gCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc < 1:819779/123‑1 (MQ=255) gCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGaa > 1:73379/1‑139 (MQ=255) ctatCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATCTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGaaa < 1:41258/136‑1 (MQ=255) tCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTc > 2:1522098/1‑139 (MQ=255) tCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTc > 2:1419057/1‑139 (MQ=255) ccACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc > 1:461551/1‑118 (MQ=255) ccACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc < 2:461551/118‑1 (MQ=255) tatTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGAcc < 1:1093324/68‑1 (MQ=255) tatTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGAcc > 2:1093324/1‑68 (MQ=255) tCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCg > 2:26603/1‑139 (MQ=255) tttACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCGGTATGTCACAACTGAATGAAAAAAGGCGGCCATTATACTGGAAATTCTGCCGCACCGTa < 2:73379/137‑1 (MQ=255) tGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACt < 2:1305369/119‑1 (MQ=255) tGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACt > 1:1305369/1‑119 (MQ=255) gCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGc > 2:1181229/1‑139 (MQ=255) aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCt > 2:791411/1‑139 (MQ=255) tatCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGGATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCcg < 1:289184/139‑1 (MQ=255) tCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGAcc < 1:1525213/48‑1 (MQ=38) tCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGAcc > 2:1525213/1‑48 (MQ=38) aaCTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGaa < 1:791411/139‑1 (MQ=255) aCTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAAc > 2:406797/1‑139 (MQ=255) cTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAag > 1:799381/1‑118 (MQ=255) cTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAag < 2:799381/118‑1 (MQ=255) ttAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGc < 2:1035512/139‑1 (MQ=255) tAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTg > 2:450291/1‑56 (MQ=39) tAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTg < 1:450291/56‑1 (MQ=39) tAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTa > 1:425281/1‑114 (MQ=255) tAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTa < 2:425281/114‑1 (MQ=255) aGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGc < 1:810221/47‑1 (MQ=38) aGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGc > 2:810221/1‑47 (MQ=38) aGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCt < 2:37514/139‑1 (MQ=255) tGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGt < 1:1522098/139‑1 (MQ=255) ccGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCta > 2:227841/1‑81 (MQ=255) ccGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCta < 1:227841/81‑1 (MQ=255) ccGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTa < 1:406797/139‑1 (MQ=255) ccGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTa < 1:26603/139‑1 (MQ=255) ggcggATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAACAg > 2:1509263/1‑72 (MQ=255) ggcggATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAg < 1:1509263/72‑1 (MQ=255) | ATGAAGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑C‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTA > NC_000913/1950416‑1950682 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |