| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,484,897 | (C)6→7 | coding (524/3594 nt) | evgS → | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,484,891 | 1 | . | C | 93.8% | 156.6 / 3.2 | 48 | coding (518/3594 nt) | evgS | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base C (23/22); total (25/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
TTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑A‑‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCA > NC_000913/2484754‑2485022 | ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑A‑‑c < 1:474815/139‑1 (MQ=255)ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTCACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑a < 2:718900/138‑1 (MQ=255)ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTCACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑a > 1:718900/1‑138 (MQ=255) cgccgcCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑A‑‑ccc > 1:632443/1‑139 (MQ=255) agacagctTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCCCCCTTCACGACTCAATGCGACCGCATACCTCGCCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑A‑‑ccccc < 1:798304/133‑1 (MQ=255) aCATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑A‑‑c > 2:1210026/1‑119 (MQ=255) aCATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑A‑‑c < 1:1210026/119‑1 (MQ=255) cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATtca > 2:639887/1‑139 (MQ=255) cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑A‑‑ccccccca > 1:477208/1‑123 (MQ=255) cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑A‑‑ccccccca < 2:477208/125‑3 (MQ=255) cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑A‑‑c < 1:1019973/118‑1 (MQ=255) cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑A‑‑c > 2:1019973/1‑118 (MQ=255) cATTGCTGCAACCAAACCATTGATAATTACATTTCCGCCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTTCTCGGGTACAAAATTAA‑‑CCCCCCAGACTAGGtctgtct > 2:798304/1‑133 (MQ=255) cAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCAt > 2:111302/1‑139 (MQ=255) accaaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCAttt > 1:614757/1‑139 (MQ=255) ttGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGca > 2:195990/1‑139 (MQ=255) gATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaac > 1:219024/1‑139 (MQ=255) tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATtcat < 1:338203/107‑1 (MQ=255) tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATtcat > 2:338203/1‑107 (MQ=255) ccGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑A‑‑ccccc < 1:131073/88‑1 (MQ=255) ccGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑A‑‑ccccc > 2:131073/1‑88 (MQ=255) gcgcTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACa < 2:219024/139‑1 (MQ=255) cTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAAt > 1:331940/1‑139 (MQ=255) gTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTta > 1:34007/1‑139 (MQ=255) aaCCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTtata < 2:34007/139‑1 (MQ=255) accagcCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat < 1:821813/139‑1 (MQ=255) caccCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑A‑‑cccccccaga < 2:167567/79‑5 (MQ=255) caccCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑A‑‑cccccccaga > 1:167567/1‑75 (MQ=255) ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATtcat > 2:928684/1‑89 (MQ=255) ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATtcat < 1:928684/89‑1 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt < 1:111302/139‑1 (MQ=255) gACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑ACCCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGc > 2:500576/1‑139 (MQ=255) gACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa < 2:778726/139‑1 (MQ=255) tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct > 2:86489/1‑139 (MQ=255) tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct > 1:477797/1‑139 (MQ=255) cGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTg > 2:622506/1‑139 (MQ=255) ttACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc < 2:477797/139‑1 (MQ=255) tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGca < 1:528350/139‑1 (MQ=255) aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACg < 2:355612/48‑1 (MQ=255) aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACg > 1:355612/1‑48 (MQ=255) cAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑A‑‑cccccccaga > 1:405269/1‑40 (MQ=255) cAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑A‑‑cccccccaga < 2:405269/44‑5 (MQ=255) aaaCCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTAc < 2:331940/139‑1 (MQ=255) aCCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa < 1:1258805/110‑1 (MQ=255) aCCAGTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa > 2:1258805/1‑110 (MQ=255) gTAAATATTGCTCGGGTAGCAAATT‑ACCCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAt < 1:500576/139‑1 (MQ=255) gTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcaca < 1:355317/125‑1 (MQ=255) gTAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcaca > 2:355317/1‑125 (MQ=255) tAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAg > 1:1530683/1‑115 (MQ=255) tAAATATTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACAAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAg < 2:1530683/115‑1 (MQ=255) atTGCTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagt < 2:1301319/139‑1 (MQ=255) cTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa < 1:679054/73‑1 (MQ=255) cTCGGGTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa > 2:679054/1‑73 (MQ=255) gTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCatcaatca < 2:399255/40‑1 (MQ=255) gTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCatcaatca > 1:399255/1‑40 (MQ=255) gTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGca < 2:900815/108‑1 (MQ=255) gTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGca > 1:900815/1‑108 (MQ=255) gTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa > 2:1375807/1‑114 (MQ=255) gTAGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa < 1:1375807/114‑1 (MQ=255) aGCAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAc < 1:639887/139‑1 (MQ=255) cAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGca > 2:623325/1‑104 (MQ=255) cAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGca < 1:623325/104‑1 (MQ=255) cAAATT‑AC‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAcaa > 2:250942/1‑137 (MQ=255) cAAATT‑A‑‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGc < 1:1511509/46‑1 (MQ=255) cAAATT‑A‑‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGc > 2:1511509/1‑46 (MQ=255) | TTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATT‑A‑‑CCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCA > NC_000913/2484754‑2485022 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |