Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,101,039	(C)7→8	coding (585/720 nt)	yggN ←	DUF2884 family putative periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,101,032	1	.	C	100.0%	153.7 / NA	44	L198V (CTG→GTG)	yggN	DUF2884 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (19/25);  total (19/25)

ATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTT  >  NC_000913/3100896‑3101160                                                                                                                                          |                                                                                                                                  aTAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑cc                                                                                                                                >  1:1129642/1‑139 (MQ=255)    gagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccc                                                                                                                             >  1:929470/1‑139 (MQ=255)    gagGAATATTTTAAACTCCCGACAAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACACCTTTGCCAAACTACGGGAAATCTTTTTCCTGCTTTTTCAACTCGGATTGGATTGAGGATGGCAG‑‑ccccc                                                                                                                             >  2:1457849/1‑139 (MQ=255)     agGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑cccccc                                                                                                                            >  1:1389538/1‑139 (MQ=255)      ggATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccccc                                                                                                                           >  2:1151861/1‑139 (MQ=255)          tattTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccccccagg                                                                                                                       <  2:682466/139‑5 (MQ=255)            ttttAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCt                                                                                                                      <  1:671807/139‑1 (MQ=255)            ttttAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑cc                                                                                                                                <  2:842979/128‑1 (MQ=255)            ttttAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑cc                                                                                                                                >  1:842979/1‑128 (MQ=255)                 aaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCa                                                                                                                 >  1:935505/1‑139 (MQ=255)                   ttCCCGGCCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGc                                                                                                               <  2:267244/139‑1 (MQ=255)                   ttCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGc                                                                                                               <  1:1151861/139‑1 (MQ=255)                       cGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACAt                                                                                                           <  1:582287/139‑1 (MQ=255)                        gACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACAtt                                                                                                          <  2:935505/139‑1 (MQ=255)                         aCCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCCAGGCTTCCCAGCACAtt                                                                                                          <  2:1129642/139‑1 (MQ=255)                                cTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccccccagg                                                                                                                       <  2:302654/117‑5 (MQ=255)                                cTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccccccagg                                                                                                                       >  1:302654/1‑113 (MQ=255)                                   tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccccccagg                                                                                                                       <  1:518299/114‑5 (MQ=255)                                   tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccccccagg                                                                                                                       >  2:518299/1‑110 (MQ=255)                                        cTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGca                                                                                                              >  2:1318596/1‑119 (MQ=255)                                        cTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGca                                                                                                              <  1:1318596/119‑1 (MQ=255)                                        cTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTaa                                                                                                    <  2:250261/129‑1 (MQ=255)                                        cTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTaa                                                                                                    >  1:250261/1‑129 (MQ=255)                                           acTTCCGGAGTCAAAACGCGGCTACAAACTTCTTGGCCAACCTGCTGGTAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGAGTGAAGC‑CCCCCCCAGGCTTCACAGCACATTCTGTAATGGGTTAccgccg                                                                                       <  1:1457849/138‑1 (MQ=255)                                                     tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGca                                                                                                              <  2:488610/106‑1 (MQ=255)                                                     tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGca                                                                                                              >  1:488610/1‑106 (MQ=255)                                                                          ctatGCCAAACGGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATCGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAt                                                        >  2:890170/4‑139 (MQ=255)                                                                          cTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAt                                                        <  1:890170/139‑1 (MQ=255)                                                                             tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGca                                                                                                              >  1:1300093/1‑82 (MQ=255)                                                                             tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGca                                                                                                              <  2:1300093/82‑1 (MQ=255)                                                                             tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTaa                                                     >  2:740215/1‑139 (MQ=255)                                                                              gCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCa                                                                             >  1:1496524/1‑114 (MQ=255)                                                                              gCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCa                                                                             <  2:1496524/114‑1 (MQ=255)                                                                               ccAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg                                                   <  2:1389538/139‑1 (MQ=255)                                                                               ccAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg                                                   <  2:929470/139‑1 (MQ=255)                                                                                  aaCTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTg                                                >  2:1175138/1‑139 (MQ=255)                                                                                       ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTg                                           >  1:637435/1‑139 (MQ=255)                                                                                              tCTTTTTCCTGCTTTTTCCACTCGGTTTGTATTGAGGATTGCAG‑‑c                                                                                                                                 <  2:1024457/45‑1 (MQ=255)                                                                                              tCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑c                                                                                                                                 >  1:1024457/1‑45 (MQ=255)                                                                                               cTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTc                                   >  2:603746/1‑139 (MQ=255)                                                                                                     ccTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCa                             <  2:637435/139‑1 (MQ=255)                                                                                                     ccTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCa                             <  1:740215/139‑1 (MQ=255)                                                                                                         cTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGc                         >  2:1464675/1‑139 (MQ=255)                                                                                                           ttttCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCt                       <  1:603746/139‑1 (MQ=255)                                                                                                            tttCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTg                      >  2:15241/1‑139 (MQ=255)                                                                                                            tttCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTg                      >  2:814768/1‑139 (MQ=255)                                                                                                             ttCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGa                     <  1:1464675/139‑1 (MQ=255)                                                                                                              tCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGca                                                                                                              >  2:513637/1‑49 (MQ=255)                                                                                                              tCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGca                                                                                                              <  1:513637/49‑1 (MQ=255)                                                                                                                   tCGGTTTGGATTGAGGATTGCAGCCCCCCCCCAGGCTTCCCAGCACAt                                                                                                           >  1:81768/1‑48 (MQ=255)                                                                                                                   tCGGTTTGGATTGAGGATTGCAGCCCCCCCCCAGGCTTCCCAGCACAt                                                                                                           <  2:81768/48‑1 (MQ=255)                                                                                                                   tCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACt               <  1:15241/139‑1 (MQ=255)                                                                                                                   tCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATCGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACt               <  1:814768/139‑1 (MQ=255)                                                                                                                       tttGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCttt                                                                                   <  2:692934/67‑1 (MQ=255)                                                                                                                       tttGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCttt                                                                                   >  1:692934/1‑67 (MQ=255)                                                                                                                                acgATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTATAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCtt  >  2:920641/3‑139 (MQ=255)                                                                                                                                 ggATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgc                                                                   <  1:859544/73‑1 (MQ=255)                                                                                                                                 ggATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgc                                                                   >  2:859544/1‑73 (MQ=255)                                                                                                                                          |                                                                                                                                  ATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTT  >  NC_000913/3100896‑3101160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑