| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,897,050 | +G | coding (277/666 nt) | yieH → | phosphoenolpyruvate and 6‑phosphogluconate phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,897,046 | 1 | . | G | 98.4% | 219.3 / ‑3.8 | 63 | coding (273/666 nt) | yieH | phosphoenolpyruvate and 6‑phosphogluconate phosphatase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (32/30); total (32/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑A‑GGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGG > NC_000913/3896909‑3897177 | ttCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑A‑g < 2:1048860/139‑1 (MQ=255) tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGa‑a > 2:150692/1‑128 (MQ=255) tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGa‑a < 1:150692/128‑1 (MQ=255) tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAg > 2:616590/1‑139 (MQ=255) aCTGTACGAAATTATCGTTATTGTTGCCCTCGAACATGGTGTTACGTTAGCGAAAACAGCAGCTGAACACGTTTACCGTGCAGAAGCAGCTCGGCTGTTCGATTCAGAACTGGGAGGCACGGATG‑GGGGCTGGATCGCt < 2:739494/139‑1 (MQ=255) gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTcc > 2:1306162/1‑138 (MQ=255) gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTcc < 1:1306162/138‑1 (MQ=255) gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTcc > 1:1334792/1‑138 (MQ=255) gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTcc < 2:1334792/138‑1 (MQ=255) tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGa > 1:1142284/1‑130 (MQ=255) tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGa < 2:1142284/130‑1 (MQ=255) tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTg > 1:1493086/1‑139 (MQ=255) tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTg > 1:92925/1‑139 (MQ=255) tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTg < 2:942277/139‑1 (MQ=255) tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTg > 1:27227/1‑139 (MQ=255) tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTg > 2:1270702/1‑139 (MQ=255) tATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGAt < 2:27227/139‑1 (MQ=255) atTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg < 1:1496012/139‑1 (MQ=255) tGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGACGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAg > 1:64266/1‑126 (MQ=255) tGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAg < 2:64266/126‑1 (MQ=255) tttCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCaa > 1:200857/1‑139 (MQ=255) tttCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑A‑gggggc < 1:853169/107‑3 (MQ=255) tttCCCTTGAACATGGTGATACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑A‑gggggc > 2:853169/1‑105 (MQ=255) ttCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGAAGCGCTCCTGTCAGCGATCACTGCGCCAAt > 2:379192/1‑139 (MQ=255) ttGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg < 2:1493086/139‑1 (MQ=255) tGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtgt > 1:497560/1‑139 (MQ=255) ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCt > 2:823663/1‑139 (MQ=255) gtgtTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑A‑g < 1:1259913/87‑1 (MQ=255) gtgtTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑A‑g > 2:1259913/1‑87 (MQ=255) aCGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGc < 1:22263/139‑1 (MQ=255) aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTcc > 1:1296884/1‑93 (MQ=255) aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTcc < 2:1296884/93‑1 (MQ=255) aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCg > 1:967429/1‑101 (MQ=255) aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCg < 2:967429/101‑1 (MQ=255) aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACt < 1:669810/107‑1 (MQ=255) aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACt > 2:669810/1‑107 (MQ=255) aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCaaa < 2:200857/139‑1 (MQ=255) cGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCGCTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATa > 1:964886/1‑139 (MQ=255) aCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACaaa > 2:1242639/1‑139 (MQ=255) cccGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCa < 2:28644/84‑1 (MQ=255) cacGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTGAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa < 2:1015848/94‑1 (MQ=255) cacGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCa > 1:28644/1‑86 (MQ=255) cacGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCAGTGCGCCa > 1:1015848/1‑94 (MQ=255) tACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc < 2:497560/139‑1 (MQ=255) gTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGc < 2:179870/139‑1 (MQ=255) gTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGc < 2:699339/139‑1 (MQ=255) cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGa < 1:616590/139‑1 (MQ=255) aaGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAata < 1:1270702/139‑1 (MQ=255) gTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATggg > 2:1234349/1‑126 (MQ=255) gTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATggg < 1:1234349/126‑1 (MQ=255) gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGAt < 1:1239614/63‑1 (MQ=255) gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGAt > 2:1239614/1‑63 (MQ=255) cTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACaaa > 2:774618/1‑105 (MQ=255) cTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACaaa < 1:774618/105‑1 (MQ=255) ggCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCact < 2:1453966/139‑1 (MQ=255) gCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCacta > 2:351694/1‑139 (MQ=255) gCTGTTCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGAAGCATTCTATGGGCAAGCTGAATATGTTGCacta > 1:983589/1‑139 (MQ=255) tCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcagca < 1:1070241/104‑1 (MQ=255) tCGATTCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcagca > 2:1070241/1‑104 (MQ=255) ttCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg < 2:374380/66‑1 (MQ=255) ttCAGAACTGGAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg > 1:374380/1‑66 (MQ=255) gAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCaaa > 1:949975/1‑75 (MQ=255) gAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCaaa < 2:949975/75‑1 (MQ=255) gAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgca < 2:477746/87‑1 (MQ=255) gAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgca > 1:477746/1‑87 (MQ=255) gAAGCCATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTc > 1:4424/1‑139 (MQ=255) cATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCaa > 2:1263988/1‑97 (MQ=255) cATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCaa < 1:1263988/97‑1 (MQ=255) cATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg > 2:743987/1‑139 (MQ=255) cATCGA‑AGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg < 2:983589/139‑1 (MQ=255) | TTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGA‑A‑GGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGG > NC_000913/3896909‑3897177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |