| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,835,045 | G→A | *378* (TAG→TAA) | norW → | NADH:flavorubredoxin oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,835,045 | 0 | G | A | 97.6% | 126.2 / ‑4.8 | 42 | *378* (TAG→TAA) | norW | NADH:flavorubredoxin oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (24/17); total (25/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
ACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AG‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTAT > NC_000913/2834926‑2835176 | aCCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTaaa > 2:792767/1‑139 (MQ=255) aaCGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGt > 1:713200/1‑139 (MQ=255) aCGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAAGTGTGGGCTACTGTGCCTAAAATg > 1:1296115/1‑139 (MQ=255) aCGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTc > 1:911258/1‑139 (MQ=255) aTGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACg < 2:911258/139‑1 (MQ=255) aTGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACg < 2:713200/139‑1 (MQ=255) gcgcgcGGCGTTGACGATGCTGACCAGCTTCCCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGAATGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGgcg > 2:258440/1‑139 (MQ=255) cgcgGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGt > 1:802669/1‑139 (MQ=255) cgcgGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGt > 2:612895/1‑139 (MQ=255) ggcgTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCtt < 1:792767/139‑1 (MQ=255) tGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTAc > 2:1038248/1‑139 (MQ=255) cAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACg < 1:612895/139‑1 (MQ=255) gCTTCGCGCCTTTGTGGTCAGTGAGGATCAGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCa < 1:1038248/139‑1 (MQ=255) cTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCAt > 1:939207/1‑139 (MQ=255) aGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCt < 1:1336997/109‑1 (MQ=255) aGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCt > 2:1336997/1‑109 (MQ=255) tGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGt > 2:304779/1‑139 (MQ=255) tGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGt > 2:1258728/1‑139 (MQ=255) tGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGACCGGATAAGGCGt > 1:234587/1‑139 (MQ=255) ggATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTa > 2:1137969/1‑139 (MQ=255) aTCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTAcg < 1:1258728/139‑1 (MQ=255) ggATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTAcgccg > 1:1512805/1‑139 (MQ=255) gATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTAcgccgc > 1:125189/1‑139 (MQ=255) aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAgg < 2:1527826/116‑1 (MQ=255) aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAgg > 1:1527826/1‑116 (MQ=255) aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCa < 1:1137969/139‑1 (MQ=255) agagGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATcc > 1:492794/1‑87 (MQ=255) agagGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATcc < 2:492794/87‑1 (MQ=255) agGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGa < 1:377422/97‑1 (MQ=255) agGCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGa > 2:377422/1‑97 (MQ=255) ggCATTTGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaa < 1:304779/139‑1 (MQ=255) ttGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGcgcg > 2:1428972/1‑71 (MQ=255) ttGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGcgcg < 1:1428972/71‑1 (MQ=255) tGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACg > 2:183874/1‑92 (MQ=255) tGGATTGTTGAAAACATTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACg < 1:183874/92‑1 (MQ=255) aTTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGAc > 2:1317755/1‑46 (MQ=255) aTTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGAc < 1:1317755/46‑1 (MQ=255) aTTGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGt > 2:484272/1‑139 (MQ=255) tGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGttt > 1:545642/1‑139 (MQ=255) tGCCGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGttt > 1:175844/1‑139 (MQ=255) cGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTAt < 1:258440/139‑1 (MQ=255) cGATGT‑AA‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTAt < 2:939207/139‑1 (MQ=255) | ACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGT‑AG‑GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTAT > NC_000913/2834926‑2835176 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |