Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,006,415	Δ1 bp	coding (464/543 nt)	zapC →	FtsZ stabilizer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,006,413	0	G	.	100.0%	205.7 / NA	50	coding (462/543 nt)	zapC	FtsZ stabilizer
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (24/26);  total (24/26)

CCGGGCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGC  >  NC_000913/1006276‑1006546                                                                                                                                          |                                                                                                                                      ccGGGCGATGCGGCCTGCGTTTGGTTGAGCGAAACTCACGTGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTTGCACAGCCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTgg                                                                                                                                      <  2:1079404/139‑1 (MQ=255)    ggCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTggcga                                                                                                                                   <  1:1248573/139‑4 (MQ=255)      cGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACg                                                                                                                                <  2:881020/139‑1 (MQ=255)                         ttGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGa                                                                                                             <  2:323529/139‑1 (MQ=255)                            aGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAg                                                                                                          >  1:1378846/1‑139 (MQ=255)                            aGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAg                                                                                                          >  1:271297/1‑139 (MQ=255)                                 tACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGa                                                                                                     >  2:684330/1‑139 (MQ=255)                                      aCGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                                >  2:581375/1‑139 (MQ=255)                                        gAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCa                                                                                              >  2:1193622/1‑139 (MQ=255)                                           cAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGt                                                                                           >  2:652533/1‑139 (MQ=255)                                           cAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGt                                                                                           >  2:5142/1‑139 (MQ=255)                                             ggTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTa                                                                                         >  2:829767/1‑139 (MQ=255)                                             ggTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTa                                                                                         <  1:581375/139‑1 (MQ=255)                                              gTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGt                                                                                           >  1:198276/1‑136 (MQ=255)                                              gTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGt                                                                                           <  2:198276/136‑1 (MQ=255)                                                   tttGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGt                                                                                           <  2:759585/131‑1 (MQ=255)                                                   tttGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGt                                                                                           >  1:759585/1‑131 (MQ=255)                                                     tGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGAc                                                                                 <  1:70483/139‑1 (MQ=255)                                                          gTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCtt                                                                            <  2:1378846/139‑1 (MQ=255)                                                          gTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCtt                                                                            <  1:829767/139‑1 (MQ=255)                                                             gTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTaaa                                                                                                                        <  2:1038153/92‑1 (MQ=255)                                                             gTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTaaa                                                                                                                        >  1:1038153/1‑92 (MQ=255)                                                             gTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAg                                                                         >  1:413982/1‑139 (MQ=255)                                                                                    aCTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAAcgcg                                                  <  1:5142/139‑1 (MQ=255)                                                                                        tGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCaa                                              >  2:659573/1‑139 (MQ=255)                                                                                               tgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGa                                                                                                             <  1:1333608/69‑1 (MQ=255)                                                                                               tgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGa                                                                                                             >  2:1333608/1‑69 (MQ=255)                                                                                                ggCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTaaaa                                                                                                                       >  1:278764/1‑58 (MQ=255)                                                                                                ggCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTaaaa                                                                                                                       <  2:278764/58‑1 (MQ=255)                                                                                                ggCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTg                                      <  2:413982/139‑1 (MQ=255)                                                                                                ggCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTg                                      <  2:1500101/139‑1 (MQ=255)                                                                                                    caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGt                                  <  1:684330/139‑1 (MQ=255)                                                                                                       cccTGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCtt                               <  1:652533/139‑1 (MQ=255)                                                                                                         cTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTgg                                                                                                                                      <  2:654186/35‑1 (MQ=255)                                                                                                         cTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTgg                                                                                                                                      >  1:654186/1‑35 (MQ=255)                                                                                                          tGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                                <  1:562737/71‑1 (MQ=255)                                                                                                          tGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                                >  2:562737/1‑71 (MQ=255)                                                                                                          tGCGTTGTTATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCgg                            <  1:1193622/139‑1 (MQ=255)                                                                                                                 ttATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGAc                                                                                 <  2:184874/79‑1 (MQ=255)                                                                                                                 ttATTGCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGAc                                                                                 >  1:184874/1‑79 (MQ=255)                                                                                                                      gCGGGTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACa                >  1:847432/1‑139 (MQ=255)                                                                                                                         ggTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTTATGTTGa                                                                                  >  1:1054273/1‑70 (MQ=255)                                                                                                                         ggTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGa                                                                                  <  2:1054273/70‑1 (MQ=255)                                                                                                                         ggTCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCa             >  1:18232/1‑139 (MQ=255)                                                                                                                           tCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCa             <  2:188963/137‑1 (MQ=255)                                                                                                                           tCGCGCGATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCa             >  1:188963/1‑137 (MQ=255)                                                                                                                              cgcgATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTg                                      >  2:712540/1‑109 (MQ=255)                                                                                                                              cgcgATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTg                                      <  1:712540/109‑1 (MQ=255)                                                                                                                              cgcgATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGt                                  <  1:10332/113‑1 (MQ=255)                                                                                                                              cgcgATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGt                                  >  2:10332/1‑113 (MQ=255)                                                                                                                              cgcgATGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGaa        <  2:257564/139‑1 (MQ=255)                                                                                                                                   tGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCtt                               >  2:332285/1‑111 (MQ=255)                                                                                                                                   tGCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCtt                               <  1:332285/111‑1 (MQ=255)                                                                                                                                    gCAGTT‑GGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGc  <  2:18232/139‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      CCGGGCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGC  >  NC_000913/1006276‑1006546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑