Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,568,105	+C	coding (3/1227 nt)	eutH ←	ethanolamine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,568,102	1	.	C	100.0%	161.4 / NA	45	G2G (GGA→GGG)	eutH	ethanolamine transporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (24/21);  total (24/21)

ACCGCCTGATCCTTCGATACTTTTACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑C‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCG  >  NC_000913/2567970‑2568233                                                                                                                                      |                                                                                                                                     aCCGCCTGATCCTTCGATACTTTTACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑c‑cccat                                                                                                                               <  2:273963/139‑4 (MQ=255)             ttCGATACTTTTACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTcc                                                                                                                    <  1:615690/139‑1 (MQ=255)                gATACTTTTACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTg                                                                                                                 >  1:573851/1‑139 (MQ=255)                gATACTTTTACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTg                                                                                                                 >  1:893226/1‑139 (MQ=255)                    cTTTTACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAg                                                                                                             <  2:36224/139‑1 (MQ=255)                     ttttACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAgt                                                                                                            >  2:810402/1‑139 (MQ=255)                       ttACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAgttg                                                                                                          >  1:1282664/1‑139 (MQ=255)                       ttACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑c‑cccat                                                                                                                               <  2:1185522/117‑4 (MQ=255)                       ttACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑c‑cccat                                                                                                                               >  1:1185522/1‑114 (MQ=255)                              aCTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑c‑cccat                                                                                                                               >  2:27023/1‑107 (MQ=255)                              aCTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑c‑cccat                                                                                                                               <  1:27023/110‑4 (MQ=255)                               cTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGttatttat                                                                                                  >  2:412093/1‑139 (MQ=255)                                        gAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑c‑cccat                                                                                                                               >  1:687564/1‑97 (MQ=255)                                        gAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑c‑cccat                                                                                                                               <  2:687564/100‑4 (MQ=255)                                          aaCGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATgcg                                                                                       >  1:218994/1‑139 (MQ=255)                                                cAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTaa                                                                                 <  2:1282664/139‑1 (MQ=255)                                                 aGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTaaa                                                                                >  1:220661/1‑139 (MQ=255)                                                 aGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTaaa                                                                                <  1:810402/139‑1 (MQ=255)                                                     ccgccgAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑c‑cccat                                                                                                                               >  2:1422652/1‑84 (MQ=255)                                                     ccgccgAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑c‑cccat                                                                                                                               <  1:1422652/87‑4 (MQ=255)                                                        ccgAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTc                                                                                                                     >  2:1104913/1‑95 (MQ=255)                                                        ccgAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTc                                                                                                                     <  1:1104913/95‑1 (MQ=255)                                                        ccgAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTa                                                                         >  2:1200128/1‑139 (MQ=255)                                                                  aCAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTAc                                                               <  1:955555/139‑1 (MQ=255)                                                                       aTCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGCTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACAtgtg                                                          <  2:470813/139‑1 (MQ=255)                                                                         ccTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCa                                                        <  1:412093/139‑1 (MQ=255)                                                                                aCGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGt                                                 <  2:218994/139‑1 (MQ=255)                                                                                  ggCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑c‑cccat                                                                                                                               <  1:1201213/58‑4 (MQ=255)                                                                                  ggCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑c‑cccat                                                                                                                               >  2:1201213/1‑55 (MQ=255)                                                                                    cAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGc                                             >  2:133028/1‑139 (MQ=255)                                                                                       cTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTgg                                          >  2:935970/1‑139 (MQ=255)                                                                                                   aGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTa                                                                                                                        >  2:1339668/1‑49 (MQ=39)                                                                                                   aGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTa                                                                                                                        <  1:1339668/49‑1 (MQ=39)                                                                                                   aGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAgtt                                                                                                           <  2:1021000/62‑1 (MQ=255)                                                                                                   aGAACATCATGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAgtt                                                                                                           >  1:1021000/1‑62 (MQ=255)                                                                                                        atcatGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCg                                                                     <  2:978316/95‑1 (MQ=255)                                                                                                        atcatGATGTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCg                                                                     >  1:978316/1‑95 (MQ=255)                                                                                                        atcatGATGTACATGATGATTTCGTTAATT‑c‑cccat                                                                                                                               <  1:1442822/36‑4 (MQ=255)                                                                                                        atcatGATGTACATGATGATTTCGTTAATT‑c‑cccat                                                                                                                               >  2:1442822/1‑33 (MQ=255)                                                                                                           ttggtgTCCATGATTATTTCGTAAATT‑CACCACAGCCTTTACTCCCTGGTAGTTGTTATTTATTGGCGAATGCGACGTAAACACCTTATCCGTGCTACATGTGCAGACCCTTAGGCTGGACAAGACGCGGCAACCGTCg                      <  1:692399/135‑1 (MQ=255)                                                                                                               tgTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTa                                                                <  1:379814/93‑1 (MQ=255)                                                                                                               tgTACATGATGATTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTa                                                                >  2:379814/1‑93 (MQ=255)                                                                                                                      gatgatTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTgg                                                                                               <  1:878328/55‑1 (MQ=255)                                                                                                                      gatgatTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTgg                                                                                               >  2:878328/1‑55 (MQ=255)                                                                                                                      gatgatTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTa                                                                                  >  2:1578075/1‑68 (MQ=255)                                                                                                                      gatgatTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTa                                                                                  <  1:1578075/68‑1 (MQ=255)                                                                                                                      gatgatTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTa                                                <  2:1108851/102‑1 (MQ=255)                                                                                                                      gatgatTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTa                                                >  1:1108851/1‑102 (MQ=255)                                                                                                                       atgatTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTAt                                                                        <  1:519324/77‑1 (MQ=255)                                                                                                                       atgatTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTAt                                                                        >  2:519324/1‑77 (MQ=255)                                                                                                                        tgatTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGc                                             <  1:38416/103‑1 (MQ=255)                                                                                                                        tgatTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGc                                             >  2:38416/1‑103 (MQ=255)                                                                                                                         gatTTCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc        >  1:888080/1‑139 (MQ=255)                                                                                                                           tttCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc      >  2:846509/1‑139 (MQ=255)                                                                                                                           tttCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc      >  2:215468/1‑139 (MQ=255)                                                                                                                            ttCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAcc     >  1:16026/1‑139 (MQ=255)                                                                                                                             tCGTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcg    <  1:846509/139‑1 (MQ=255)                                                                                                                               gTTAATTCC‑CCATCGCCTTTCCTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgcg  <  2:1198039/139‑1 (MQ=255)                                                                                                                               gTTAATTCC‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgcg  <  1:215468/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     ACCGCCTGATCCTTCGATACTTTTACCGAACTTACCGAGGAAACGAGCAGAACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATT‑C‑CCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCG  >  NC_000913/2567970‑2568233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑