| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,767,445 | (C)7→8 | intergenic (+90/‑265) | rnlB → / → yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,767,438 | 1 | . | C | 85.7% | 138.4 / 14.8 | 49 | intergenic (+83/‑272) | rnlB/yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
| Reads supporting (aligned to +/- strand): ref base . (7/0); new base C (17/25); total (24/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.03e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
CCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AA‑C‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGG > NC_000913/2767303‑2767571 | ccTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AA‑c‑c‑c < 1:1563446/139‑1 (MQ=255) tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AA‑c‑c‑cc‑c > 2:1443495/1‑139 (MQ=255) ctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACTAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AA‑c‑c‑cc‑cc < 2:1005280/139‑1 (MQ=255) ctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AA‑cttccg > 1:1185992/1‑134 (MQ=255) gTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTcaca < 2:866079/139‑1 (MQ=255) cccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGt > 2:840207/1‑139 (MQ=255) ccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTc < 1:840207/139‑1 (MQ=255) atgTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCAt > 2:578136/1‑139 (MQ=255) ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAac < 2:1036833/139‑1 (MQ=255) ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAac < 1:578136/139‑1 (MQ=255) cAAGTCCTGTCAATGGATTTTTGAATCTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AA‑c < 1:1254873/108‑1 (MQ=255) cAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGt > 2:1580537/1‑124 (MQ=255) cAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGt < 1:1580537/124‑1 (MQ=255) cAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AA‑c > 2:1254873/1‑108 (MQ=255) cAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AA‑C‑C‑CCTCCCCCAAAACGTACTGCATAGGAAacc > 2:425657/1‑138 (MQ=255) gTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑TAAA‑C‑C‑CC‑CCCACACAAAATACTGCATATTGAacaaga > 2:508736/1‑136 (MQ=255) cTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGtttt < 1:810650/139‑1 (MQ=255) gTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACTACGTCCTGCATAgggtat > 2:115014/1‑127 (MQ=255) gTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAac < 1:115014/130‑1 (MQ=255) cAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTcccc < 2:112751/139‑1 (MQ=255) ggATTTTTGAAACTCTTCTTTCTAAACGAACTAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AA‑c < 1:1315783/94‑1 (MQ=255) ggATTTTTGAAACTCTTCTTTCTAAACGAACTAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AA‑c > 2:1315783/1‑94 (MQ=255) ggATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCtgtg > 2:71632/1‑139 (MQ=255) ggATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AA‑c‑c‑cc > 1:300676/1‑97 (MQ=255) ggATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AA‑c‑c‑cc < 2:300676/97‑1 (MQ=255) ttttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc < 1:238736/139‑1 (MQ=255) ttttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc < 1:124418/139‑1 (MQ=255) ttttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc < 1:71632/139‑1 (MQ=255) tttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑TAAA‑A‑A‑CC‑CCATCACAACGACCTGCAAGAGGAACAGTATTGCCCTTATGCAccc > 1:1184843/1‑139 (MQ=255) tGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCCCAACGTCCTGCATAGGGAACACGtttt > 1:1297410/1‑124 (MQ=255) tGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGtttt < 2:1297410/124‑1 (MQ=255) tttctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTc < 2:1185992/139‑1 (MQ=255) tctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTcc < 1:1266721/92‑1 (MQ=255) tctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTcc > 2:1266721/1‑92 (MQ=255) ccAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTaaa < 2:1184843/139‑1 (MQ=255) caggTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTaaa < 2:242928/136‑1 (MQ=255) gTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACtt < 2:686477/139‑1 (MQ=255) ttCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAac < 2:125002/83‑1 (MQ=255) ttCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAac > 1:125002/1‑83 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTcaca < 2:626845/62‑1 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTcaca > 1:626845/1‑62 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTc > 2:112540/1‑88 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTc < 1:112540/88‑1 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTATGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGt > 2:830091/1‑139 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACATCCTGCATAGGGAACACGTTTTCACCCGTGCACCCACGACTAAATCACCCCCCCTTTAACTTTCTCaataactt > 1:242928/1‑136 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AA‑CTT‑CC‑TCCTGACAACGCCCTGAATAGGGTTCTCGATGTCCACTGTGCACCCAACACAAATTGTCGTCCCCATTAAACTTATCtatcagct > 2:1374398/1‑134 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AA‑C‑CACC‑CCCTCACAACGTCCCGCATCAGGATAACCTTTTCCCCTGTGCACACACGACTAAATTTCCCCCCCTTTAGACTTCTCatgcatga > 1:1119716/1‑132 (MQ=255) gCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAAttt < 1:1374398/139‑1 (MQ=255) atatTAACTTGTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTgg < 2:1119716/139‑1 (MQ=255) aaCTTGTCCAATATGATCAAATAGCATAA‑AA‑C‑C‑AT‑CCCTCACTCCCTCCTGTATAGGGATCACGGATTCCCATTCGCATAAACGACTATTTTTCCCTCCCTATATACTTCTCAAAAATGTCACCAATTATCTGAAAATc > 2:201689/1‑139 (MQ=255) gTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTccc < 2:1094855/66‑1 (MQ=255) gTCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTccc > 1:1094855/1‑66 (MQ=255) tCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTc > 2:689117/1‑42 (MQ=255) tCCAATATGATCAAATAGCAT‑T‑AACC‑C‑CC‑CCCTCACAACGTc < 1:689117/42‑1 (MQ=255) tCCAATATGATCAAATAGCAT‑T‑AA‑C‑CTGC‑CCCTCACAACGTCCTGCATAGAGAACACGTTTTCACCTGTGCACCCACGCATAAATTTCCACACCTTTAAACTTCTCAATAATGTCACCAATCTACTGGAAATCCTacga > 2:1036005/1‑139 (MQ=255) tCAAATAGCAT‑T‑AA‑CAC‑CC‑CCCTCACAACGTCCTGCATTGGGAACACGCGTTACCCTGTGCACCCACGACTAAAATTCCCCTCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGTAAATCCTATTATGCCTTTcac > 1:315630/1‑137 (MQ=255) cAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgat > 1:979315/1‑122 (MQ=255) cAT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgat < 2:979315/122‑1 (MQ=255) aT‑T‑AACC‑C‑CC‑CCCTCACAATGTCCTGCATAGGGAACACGTTTTCACCTGTGCACCCACGACTAAATTTCCCCCGCTTTAAACTTCTCAATACTGTCACCAATTTTCTGGAACTCCTATGATCCCTTTCCCTGTCAATgg > 1:1140860/1‑139 (MQ=255) aT‑T‑AACC‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTTCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCCGTAAATgg > 2:1531916/1‑139 (MQ=255) | CCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCAT‑T‑AA‑C‑C‑CC‑CCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGG > NC_000913/2767303‑2767571 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |