| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,330,357 | +T | coding (1395/1434 nt) | dacB → | D‑alanyl‑D‑alanine carboxypeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,330,353 | 1 | . | T | 98.3% | 191.8 / ‑4.0 | 60 | coding (1391/1434 nt) | dacB | D‑alanyl‑D‑alanine carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (27/32); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑G‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATC > NC_000913/3330229‑3330485 | ggggTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCTTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt < 2:1077432/139‑1 (MQ=255)ggggTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt < 1:173706/139‑1 (MQ=255)ggggTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt < 2:234436/139‑1 (MQ=255) aCCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt > 1:458216/1‑129 (MQ=255) aCCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt > 1:476631/1‑129 (MQ=255) aCCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt < 2:458216/129‑1 (MQ=255) aCCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt < 2:476631/129‑1 (MQ=255) ggCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATaa < 1:1107641/133‑1 (MQ=255) ggCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATaa > 2:1107641/1‑133 (MQ=255) ggCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGatat > 1:1105552/1‑139 (MQ=255) cGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt > 2:82490/1‑123 (MQ=255) cGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt < 1:82490/123‑1 (MQ=255) cGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATAttt > 2:1204582/1‑139 (MQ=255) ggATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTAt < 1:2639/139‑1 (MQ=255) caGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGtat > 1:104608/1‑114 (MQ=255) caGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGtat < 2:104608/114‑1 (MQ=255) agcgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATaa > 2:734752/1‑115 (MQ=255) agcgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATaa < 1:734752/115‑1 (MQ=255) agcgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTaatc > 1:594745/1‑136 (MQ=255) cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑G‑tttttgaa > 2:1390971/1‑95 (MQ=255) cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑G‑tttttgaa < 1:1390971/99‑5 (MQ=255) agcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCaaaa > 2:838174/1‑139 (MQ=255) agcgGGCAACGAATGGCGTTTGTGCAATATC‑TTCTGCCTATGCAGTATAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGG‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCaaaaa > 2:1024123/1‑139 (MQ=255) cgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAg > 2:794868/1‑139 (MQ=255) cgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAg > 1:1181595/1‑139 (MQ=255) gggCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa < 1:1204582/139‑1 (MQ=255) gggCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑G‑tttttgaa < 2:816384/94‑5 (MQ=255) gggCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑G‑tttttgaa > 1:816384/1‑90 (MQ=255) aaTGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCgg < 2:594745/139‑1 (MQ=255) ggCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac > 1:510083/1‑139 (MQ=255) gCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt > 1:601551/1‑88 (MQ=255) gCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt < 2:601551/88‑1 (MQ=255) gCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcaca < 2:1503572/139‑1 (MQ=255) gTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑G‑tttttgaaa < 1:876023/81‑6 (MQ=255) gTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑G‑tttttgaaa > 2:876023/1‑76 (MQ=255) tgCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCgg < 1:1234999/139‑1 (MQ=255) cAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCgggg < 1:838174/139‑1 (MQ=255) tGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGattat < 2:510083/139‑1 (MQ=255) gCTATGCAGTAGAACCTGCGGATCAACGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt < 1:575256/66‑1 (MQ=255) gCTATGCAGTAGAACCTGCGGATCAACGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt > 2:575256/1‑66 (MQ=255) tGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACaa > 1:864898/1‑139 (MQ=255) aGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTgcg < 2:1105552/139‑1 (MQ=255) gAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCg > 2:796492/1‑112 (MQ=255) gAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCg < 1:796492/112‑1 (MQ=255) aaCCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGata < 2:265471/67‑1 (MQ=255) aaCCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGata > 1:265471/1‑67 (MQ=255) aaCCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa < 2:678333/93‑1 (MQ=255) aaCCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa > 1:678333/1‑93 (MQ=255) ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCttt > 2:1204453/1‑139 (MQ=255) tGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATaa > 1:1191810/1‑58 (MQ=255) tGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATaa < 2:1191810/58‑1 (MQ=255) cAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCgg < 1:1499174/101‑1 (MQ=255) cAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGCCCgg > 2:1499174/1‑101 (MQ=255) cGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTa < 2:729373/69‑1 (MQ=255) cGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTa > 1:729373/1‑69 (MQ=255) tAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATg < 1:1024123/139‑1 (MQ=255) tAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATg < 2:864898/139‑1 (MQ=255) tCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt > 2:1134202/1‑35 (MQ=37) tCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt < 1:1134202/35‑1 (MQ=37) tCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAg > 2:1372688/1‑45 (MQ=38) tCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAg < 1:1372688/45‑1 (MQ=38) ttCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGca > 1:1423618/1‑76 (MQ=255) ttCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGca < 2:1423618/76‑1 (MQ=255) ttAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa > 1:434817/1‑61 (MQ=255) ttAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa < 2:434817/61‑1 (MQ=255) aGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc < 1:166526/139‑1 (MQ=255) | GGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑G‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATC > NC_000913/3330229‑3330485 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |