| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,761,518 | G→A | R92C (CGT→TGT) | sufD ← | component of SufBCD Fe‑S cluster assembly scaffold |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,761,518 | 0 | G | A | 98.0% | 156.8 / ‑6.9 | 50 | R92C (CGT→TGT) | sufD | component of SufBCD Fe‑S cluster assembly scaffold |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (22/27); minor base C (1/0); total (23/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTA > NC_000913/1761384‑1761639 | gCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCa < 1:977603/139‑1 (MQ=255) ggCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAacac > 2:175675/1‑139 (MQ=255) tCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGCAACACCCATCGACAAACACCAGcc > 2:902138/1‑139 (MQ=255) tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCAc < 1:733098/139‑1 (MQ=255) cAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCt < 2:926267/139‑1 (MQ=255) ttCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAAc > 2:24664/1‑139 (MQ=255) gAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGc > 2:607930/1‑139 (MQ=255) cGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg > 2:165191/1‑139 (MQ=255) cGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg < 1:175675/139‑1 (MQ=255) ggTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctgc > 1:1296002/1‑139 (MQ=255) ggTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCACTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctgct < 2:1587340/139‑1 (MQ=255) aCGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg > 1:5436/1‑123 (MQ=255) aCGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg < 2:5436/123‑1 (MQ=255) aaTGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCAc < 2:877154/81‑1 (MQ=255) aaTGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCAc > 1:877154/1‑81 (MQ=255) tGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCt > 2:192464/1‑139 (MQ=255) cACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctgct < 2:271814/110‑1 (MQ=255) cACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctgct > 1:271814/1‑110 (MQ=255) cACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACa > 2:1144141/1‑139 (MQ=255) cTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctg < 2:411102/106‑1 (MQ=255) cTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctg > 1:411102/1‑106 (MQ=255) cTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa < 1:1144141/139‑1 (MQ=255) cTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa < 2:257115/139‑1 (MQ=255) ttCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAAc < 1:902138/139‑1 (MQ=255) ttCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAAc < 2:1312263/139‑1 (MQ=255) cGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTccc > 1:519617/1‑116 (MQ=255) cGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTccc < 2:519617/116‑1 (MQ=255) cTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCt < 2:970612/69‑1 (MQ=255) cTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCt > 1:970612/1‑69 (MQ=255) cTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCaa < 2:508570/118‑1 (MQ=255) cTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCaa > 1:508570/1‑118 (MQ=255) cTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt > 1:770346/1‑130 (MQ=255) cTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt < 2:770346/130‑1 (MQ=255) cTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCt < 2:510410/134‑1 (MQ=255) cTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCt > 1:510410/1‑134 (MQ=255) cTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGa > 2:1522054/1‑139 (MQ=255) tGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTAACAAACTGGCTATTGAt < 1:165191/139‑1 (MQ=255) ttCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTa > 1:465544/1‑65 (MQ=255) ttCAGTTGCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTa < 2:465544/65‑1 (MQ=255) gCATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCa < 2:1354466/139‑1 (MQ=255) cATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACg > 2:510938/1‑50 (MQ=255) cATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACg < 1:510938/50‑1 (MQ=255) cATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt > 1:1436978/1‑117 (MQ=255) cATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt < 2:1436978/117‑1 (MQ=255) cATCGCTCAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAg < 1:607930/139‑1 (MQ=255) cAGTGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGta < 1:1522054/139‑1 (MQ=255) tGC‑GGGCACGTAACCCCCATCGACAAACACCAGCCGCAc > 2:803519/1‑39 (MQ=255) tGC‑GGGCACGTAACACCCATCGACAAACACCAGCCGCAc < 1:803519/39‑1 (MQ=255) tAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCAt > 2:639516/1‑55 (MQ=255) tAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCAt < 1:639516/55‑1 (MQ=255) | GCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGC‑GGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTA > NC_000913/1761384‑1761639 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |