Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,330,396	G→A	*478* (TAG→TAA)	dacB →	D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,330,396	0	G	A	100.0%	188.7 / NA	58	*478* (TAG→TAA)	dacB	D‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/29);  total (29/29)

AGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑G‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTG  >  NC_000913/3330265‑3330529                                                                                                                                      |                                                                                                                                      agcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCaaaa                                                                                                                                 >  2:838174/1‑139 (MQ=255) agcgGGCAACGAATGGCGTTTGTGCAATATC‑TTCTGCCTATGCAGTATAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGG‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCaaaaa                                                                                                                                >  2:1024123/1‑139 (MQ=255)   cgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAg                                                                                                                               >  1:1181595/1‑139 (MQ=255)   cgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAg                                                                                                                               >  2:794868/1‑139 (MQ=255)    gggCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa                                                                                                                              <  1:1204582/139‑1 (MQ=255)            aaTGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCgg                                                                                                                      <  2:594745/139‑1 (MQ=255)               ggCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac                                                                                                                   >  1:510083/1‑139 (MQ=255)                gCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcaca                                                                                                                  <  2:1503572/139‑1 (MQ=255)                       tgCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCgg                                                                                                           <  1:1234999/139‑1 (MQ=255)                         cAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCgggg                                                                                                         <  1:838174/139‑1 (MQ=255)                                    tGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGattat                                                                                              <  2:510083/139‑1 (MQ=255)                                          tGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACaa                                                                                        >  1:864898/1‑139 (MQ=255)                                                aGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTgcg                                                                                  <  2:1105552/139‑1 (MQ=255)                                                 gAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCg                                                                                                            >  2:796492/1‑112 (MQ=255)                                                 gAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCg                                                                                                            <  1:796492/112‑1 (MQ=255)                                                  aaCCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa                                                                                                                              <  2:678333/93‑1 (MQ=255)                                                  aaCCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa                                                                                                                              >  1:678333/1‑93 (MQ=255)                                                    ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCttt                                                                              >  2:1204453/1‑139 (MQ=255)                                                             cAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCgg                                                                                                           <  1:1499174/101‑1 (MQ=255)                                                             cAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGCCCgg                                                                                                           >  2:1499174/1‑101 (MQ=255)                                                                  tAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATg                                                                <  1:1024123/139‑1 (MQ=255)                                                                  tAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATg                                                                <  2:864898/139‑1 (MQ=255)                                                                             ttCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGca                                                                                                                    <  2:1423618/76‑1 (MQ=255)                                                                             ttCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGca                                                                                                                    >  1:1423618/1‑76 (MQ=255)                                                                                  ttAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa                                                                                                                              >  1:434817/1‑61 (MQ=255)                                                                                  ttAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa                                                                                                                              <  2:434817/61‑1 (MQ=255)                                                                                    aGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc                                              <  1:166526/139‑1 (MQ=255)                                                                                           tttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                        >  1:463415/2‑139 (MQ=255)                                                                                                      cGTTTGTATAAAGATCTTTATCAGCACAATTAATCAAAATGAAACGCCGGCACATGGCCGGGGCTTCAGATGATTGACAAAGTGCGCTTTGATTATGCCGCATGCGGCATAAACACCTTATCCGGCCTCCAAAATAGTg                             >  1:1091957/1‑139 (MQ=25)                                                                                                       gTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc                            <  2:463415/139‑1 (MQ=255)                                                                                                       gTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc                            <  1:794868/139‑1 (MQ=255)                                                                                                       gTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc                            <  2:1181595/139‑1 (MQ=255)                                                                                                        tttGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCa                           >  2:596537/1‑139 (MQ=255)                                                                                                            tataAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCa                           >  1:1586227/1‑135 (MQ=255)                                                                                                            tataAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCa                           <  2:1586227/135‑1 (MQ=255)                                                                                                                aaGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac                                                                                                                   <  2:1097993/43‑1 (MQ=255)                                                                                                                aaGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac                                                                                                                   >  1:1097993/1‑43 (MQ=255)                                                                                                                aaGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAc                   >  1:115447/1‑139 (MQ=255)                                                                                                                  gATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCg                                                                                                            >  2:898349/1‑48 (MQ=255)                                                                                                                  gATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCg                                                                                                            <  1:898349/48‑1 (MQ=255)                                                                                                                      tttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc                            <  2:1401291/124‑1 (MQ=255)                                                                                                                      tttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc                            >  1:1401291/1‑124 (MQ=255)                                                                                                                      tttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc                            >  2:168907/1‑124 (MQ=255)                                                                                                                      tttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc                            <  1:168907/124‑1 (MQ=255)                                                                                                                      tttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt                       >  1:1125277/1‑129 (MQ=255)                                                                                                                      tttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt                       <  2:1125277/129‑1 (MQ=255)                                                                                                                      tttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTg             >  2:1454529/1‑139 (MQ=255)                                                                                                                      tttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTg             >  1:1045912/1‑139 (MQ=255)                                                                                                                           cAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTAGTTTATCCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAAtt        >  2:144194/1‑139 (MQ=255)                                                                                                                             gAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt                                                                                <  1:1598985/65‑1 (MQ=255)                                                                                                                             gAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt                                                                                >  2:1598985/1‑65 (MQ=255)                                                                                                                              aaCAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTgcgc                                                                                 <  1:1378278/63‑1 (MQ=255)                                                                                                                              aaCAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTgcgc                                                                                 >  2:1378278/1‑63 (MQ=255)                                                                                                                              aaCAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                                         <  2:1010915/87‑1 (MQ=255)                                                                                                                              aaCAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                                         >  1:1010915/1‑87 (MQ=255)                                                                                                                                 aaTTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTg  <  1:1454529/139‑1 (MQ=255)                                                                                                                                   ttAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc                                              <  1:1316558/93‑1 (MQ=255)                                                                                                                                   ttAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc                                              >  2:1316558/1‑93 (MQ=255)                                                                                                                                      |                                                                                                                                      AGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑G‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTG  >  NC_000913/3330265‑3330529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑