Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,208,534	Δ1 bp	coding (403/414 nt);coding (586/603 nt)	tfaP → tfaE ←	e14 prophage, uncharacterized protein e14 prophage, putative tail fiber assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,208,534	0	C	.	100.0%	228.3 / NA	61	coding (403/414 nt) coding (586/603 nt)	tfaP tfaE	e14 prophage, uncharacterized protein e14 prophage, putative tail fiber assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (33/28);  total (33/28)

GCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAATTTCAGTTGAA  >  NC_000913/1208405‑1208662                                                                                                                                  |                                                                                                                                 gCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATg                                                                                                                        >  2:918152/1‑139 (MQ=255)     cATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACAt                                                                                                                    >  1:593931/1‑139 (MQ=255)     cATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACAt                                                                                                                    >  1:1048473/1‑139 (MQ=255)     cATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACAt                                                                                                                    >  1:31534/1‑139 (MQ=255)           gAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg                                                                                                              <  1:984561/139‑1 (MQ=255)           gAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg                                                                                                              <  1:918152/139‑1 (MQ=255)             aGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGcgcg                                                                                                            <  1:459353/139‑1 (MQ=255)               aCTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGcc                                                                                                                             <  2:786657/120‑1 (MQ=255)               aCTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGcc                                                                                                                             >  1:786657/1‑120 (MQ=255)               aCTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGt                                                                                                          <  2:1048473/139‑1 (MQ=255)                cTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGCTTATCTGGAAGCACTGGAACTGGCTGATACATCCAGTGCGCCAGATATTGAATGTCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTg                                                                                                         <  1:717936/139‑1 (MQ=18)                    aagaagATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTgg                                                                                                     >  1:850235/1‑139 (MQ=255)                         gaTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCt                                                                                                <  2:31534/139‑1 (MQ=255)                          aTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTg                                                                                               >  1:367814/1‑139 (MQ=255)                          aTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTg                                                                                               <  1:628549/139‑1 (MQ=255)                           tGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGTAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGt                                                                                              >  1:76751/1‑139 (MQ=255)                                        tataATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACcgcg                                                                                 >  1:919729/1‑139 (MQ=255)                                         ataATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGt                                                                                <  2:677984/139‑1 (MQ=255)                                         ataATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGt                                                                                <  1:750397/139‑1 (MQ=255)                                                  ggCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg                                                                                                              >  2:1029659/1‑100 (MQ=255)                                                  ggCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg                                                                                                              <  1:1029659/100‑1 (MQ=255)                                                  ggCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAAt                                                                       >  2:291022/1‑139 (MQ=255)                                                             cTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCg                                                            <  2:919729/139‑1 (MQ=255)                                                                 aaGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACcg                                                                                   <  1:964328/112‑1 (MQ=255)                                                                 aaGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACcg                                                                                   >  2:964328/1‑112 (MQ=255)                                                                  aGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                                           >  2:340486/1‑119 (MQ=255)                                                                  aGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                                           <  1:340486/119‑1 (MQ=255)                                                                   gCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCAc                                                                                     <  2:369437/108‑1 (MQ=255)                                                                   gCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCAc                                                                                     >  1:369437/1‑108 (MQ=255)                                                                   gCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGt                                                      <  2:76751/139‑1 (MQ=255)                                                                     aCTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg                                                                                                              <  2:942448/81‑1 (MQ=17)                                                                     aCTGGAACTGGTTGATACATACAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg                                                                                                              >  1:942448/1‑81 (MQ=17)                                                                      cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcgc                                                                                                             <  2:971258/81‑1 (MQ=17)                                                                      cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcgc                                                                                                             >  1:971258/1‑81 (MQ=255)                                                                      cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTaa                                                                        <  2:1004595/118‑1 (MQ=255)                                                                      cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTaa                                                                        >  1:1004595/1‑118 (MQ=255)                                                                      cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTg                                                   >  2:1003903/1‑139 (MQ=255)                                                                         gAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGgtt                                                >  1:87859/1‑139 (MQ=255)                                                                              ggTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTAAATGTAATCCAGCACAGCg                                                            <  1:716368/122‑1 (MQ=255)                                                                              ggTTGATACATCCAGCGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGATGCCGTCACCGCGTCAATGTAATCCAGCACAGCg                                                            >  2:716368/1‑122 (MQ=14)                                                                               gTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCt                                                                                                >  2:580102/1‑85 (MQ=14)                                                                               gTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCt                                                                                                <  1:580102/85‑1 (MQ=14)                                                                               gTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTct                                          >  2:709820/1‑139 (MQ=255)                                                                                    tACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTctgcctg                                     >  1:106299/1‑139 (MQ=255)                                                                                       aTCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGt                                  <  2:850235/139‑1 (MQ=255)                                                                                        tCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTc                                 >  1:435776/1‑139 (MQ=255)                                                                                          cAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCa                                                                                      >  1:96839/1‑84 (MQ=11)                                                                                          cAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCa                                                                                      <  2:96839/84‑1 (MQ=11)                                                                                              gcgcCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg                                                                                                              >  1:186295/1‑56 (MQ=14)                                                                                              gcgcCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg                                                                                                              <  2:186295/56‑1 (MQ=14)                                                                                               cgcCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGca                                                                 <  2:698282/100‑1 (MQ=255)                                                                                               cgcCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGca                                                                 >  1:698282/1‑100 (MQ=255)                                                                                                 ccAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACcgc                                                                                  <  1:410860/81‑1 (MQ=255)                                                                                                 ccAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACcgc                                                                                  >  2:410860/1‑81 (MQ=11)                                                                                                 ccAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                                           >  2:680865/1‑88 (MQ=11)                                                                                                 ccAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                                           <  1:680865/88‑1 (MQ=11)                                                                                                 ccAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGt                        >  1:45041/1‑139 (MQ=255)                                                                                                               gcttACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAAttt          >  2:543417/4‑139 (MQ=255)                                                                                                                cttACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAATTTc         >  2:676028/3‑139 (MQ=255)                                                                                                                cttACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAATTTc         >  2:1060307/3‑139 (MQ=255)                                                                                                                       cTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAATTTCAGTTGaa  <  2:435776/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                 GCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAATTTCAGTTGAA  >  NC_000913/1208405‑1208662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑