| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,709,561 | (C)6→7 | coding (915/1692 nt) | eptB ← | KDO phosphoethanolamine transferase, Ca(2+)‑inducible |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,709,555 | 1 | . | C | 97.5% | 128.7 / ‑3.3 | 41 | G307G (GGC→GGG) | eptB | KDO phosphoethanolamine transferase, Ca(2+)‑inducible |
| Reads supporting (aligned to +/- strand): ref base . (0/1); major base C (20/19); minor base A (0/1); total (20/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.40e-01 | |||||||||||
GTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAA > NC_000913/3709426‑3709682 | gtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccctg < 1:157135/139‑4 (MQ=255) tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATTCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccctga > 1:733117/1‑135 (MQ=255) cATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTAc > 1:1075514/1‑139 (MQ=255) aTCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACa < 2:540318/139‑1 (MQ=255) gTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGACCCCCC‑TGACGTACAAACATGCAACg < 1:221238/139‑1 (MQ=255) caTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGagag > 2:969076/1‑139 (MQ=255) tttCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccc > 1:817968/1‑105 (MQ=255) tttCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccc < 2:817968/105‑1 (MQ=255) cGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACATACAAACATGCAACGCAGTGAGAGTTTgg < 1:86546/139‑1 (MQ=255) gCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGt < 1:285094/139‑1 (MQ=255) tctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTg < 1:338984/139‑1 (MQ=255) ctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGGTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc < 1:570509/139‑1 (MQ=255) gCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg < 1:790056/139‑1 (MQ=255) cGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac > 2:171471/1‑139 (MQ=255) cGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac > 1:20360/1‑139 (MQ=255) agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAgg > 1:113886/1‑139 (MQ=255) agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAg > 1:78951/1‑139 (MQ=255) aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca < 2:682814/128‑1 (MQ=255) aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca > 1:682814/1‑128 (MQ=255) tGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGa > 1:463604/1‑139 (MQ=255) aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAc > 2:805563/1‑139 (MQ=255) tAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGt < 1:526167/106‑1 (MQ=255) tAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGt > 2:526167/1‑106 (MQ=255) aCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCa < 1:554959/139‑1 (MQ=255) gCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg < 2:87122/71‑1 (MQ=255) gCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg > 1:87122/1‑71 (MQ=255) tCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc < 1:1003556/62‑1 (MQ=255) tCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc > 2:1003556/1‑62 (MQ=255) aaCCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTtctc > 2:184387/1‑139 (MQ=255) gcgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg < 1:149172/60‑1 (MQ=255) gcgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg > 2:149172/1‑60 (MQ=255) cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGTGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTggg > 1:172155/1‑139 (MQ=255) cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTggg > 1:1054990/1‑139 (MQ=255) cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGCTTGGTTGCGGTATCACAGGAGTACCCACGGAACGCCGCCAGATTTTTCTCCTggg > 2:245027/1‑139 (MQ=255) gttctgttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGttt < 2:463604/139‑1 (MQ=255) tctgttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCg < 1:805563/139‑1 (MQ=255) ctgttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCgg < 2:588570/139‑1 (MQ=255) gttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg < 1:278531/139‑1 (MQ=255) ttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGTTTGCGTTATca > 2:961932/1‑86 (MQ=255) ttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca < 1:961932/86‑1 (MQ=255) tctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTg < 2:113886/139‑1 (MQ=255) tctTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCCATGACGTACAAACATGCAACGCAGTGAGAGTTTGATTGCGGTATCACAGGAGTAACCACGTATCGCCGCCAGAGTTTTCTCCTGGGCCAGTTTCCGCGTg < 2:733117/139‑1 (MQ=255) ttAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccc < 2:493320/36‑2 (MQ=255) ttAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccc > 1:493320/1‑35 (MQ=255) cgcTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAAc > 2:703271/1‑47 (MQ=255) cgcTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAAc < 1:703271/47‑1 (MQ=255) cGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGcc > 1:858179/1‑139 (MQ=255) aTTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGTGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGaa < 2:172155/139‑1 (MQ=255) tATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGaaaa > 1:163485/1‑139 (MQ=255) | GTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAA > NC_000913/3709426‑3709682 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |