| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 380,022 | (G)10→11 | intergenic (‑141/+47) | frmR ← / ← yaiO | regulator protein that represses frmRAB operon/outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 380,012 | 1 | . | G | 77.8% | 141.9 / 35.7 | 54 | intergenic (‑131/+57) | frmR/yaiO | regulator protein that represses frmRAB operon/outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (6/6); new base G (28/14); total (34/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCC > NC_000913/379877‑380150 | ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑ggg > 2:129752/1‑139 (MQ=255) ttCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑gggggg‑‑gg > 1:808158/1‑139 (MQ=255) aCCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGG‑‑GGGG‑CTa < 2:808158/139‑1 (MQ=255) catgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGaa > 1:3/1‑139 (MQ=255) tgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGG‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAg > 1:170357/1‑139 (MQ=255) tataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGa > 1:232769/1‑139 (MQ=255) taGGGGGGTATTCTATATGTCAATGCATACCCCCCGATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGG‑GGGGGG‑‑GGGG‑TTAA‑TCGGCAGGGAAGGCCAcccgagat > 1:787446/1‑134 (MQ=255) aGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGG‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATa > 1:1008234/1‑139 (MQ=255) aGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAg > 1:851523/1‑139 (MQ=255) gggggTCTTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGTGAAGGCCGCCCCGGATAgcgg > 2:184508/1‑139 (MQ=255) gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGTTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAgcggg < 2:232769/139‑1 (MQ=255) gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAgcggg > 2:856601/1‑139 (MQ=255) gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAgcggg > 1:304582/1‑139 (MQ=255) gTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAgg > 2:77548/1‑120 (MQ=255) gTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAgg < 1:77548/120‑1 (MQ=255) tatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg > 2:324567/1‑139 (MQ=255) cAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAg > 2:487147/1‑139 (MQ=255) tatataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGG‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACgg > 1:79042/1‑139 (MQ=255) tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt > 2:864147/1‑139 (MQ=255) tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑g < 1:426492/58‑1 (MQ=255) tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑g > 2:426492/1‑58 (MQ=255) tataAAACTGGAGAATAAAATTTATCCGGTGAAGGGGGTCGGAAATCAAAGAGGAAA‑‑‑GGGGGGC‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGTGCt < 2:691022/139‑1 (MQ=255) ataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTg > 1:869571/1‑139 (MQ=255) aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAGAGAGGAAAGG‑GGGGGG‑‑GGGG‑CAAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGGGGCAGAAGGAATCAAAATTTCCAGGTCAGACGGGCTGc > 1:146803/1‑139 (MQ=255) aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCa > 2:544315/1‑139 (MQ=255) aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCaa > 2:282718/1‑139 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACggg > 2:183478/1‑132 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACggg < 1:183478/132‑1 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg > 2:412206/1‑139 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGG‑GGGGGG‑‑GGGG‑GTAA‑TCGGAAGGGAAGGCCGCCCCGGAAAGGGGGCGGCAGAAGGAATAAGAATTTCCGGGTAAGACGGGCGGCAGGtg > 2:414457/1‑138 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGa < 2:272580/101‑1 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGa > 1:272580/1‑101 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg > 1:691022/1‑139 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg > 1:511645/1‑139 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg > 2:85568/1‑139 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGG‑‑GGGGCCTAA‑TCGCCAGGGAAGGCCCCCCCGGTAAGCGGGGGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg > 1:381457/1‑139 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCgg > 2:430251/1‑66 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCgg < 1:430251/66‑1 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa < 1:324567/139‑1 (MQ=255) agaAAAAAATTTATCCGGTGAATGGGGTCGGAAAACAAAGAGGAAAGG‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa < 2:146803/139‑1 (MQ=255) aaTAAAATTTTTCCGGGGAAGGGTGTCGGAAAACAAAGGGGAAAGG‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa < 2:79042/139‑1 (MQ=255) tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGG‑‑GGGG‑CAAA‑ACGGGAGGGAAGGCCGCCCCGGGTAGGGGGGGGGAAAAGGGAACAGAATTTCCAGGTCAGACGGGCTGCAAGTTGTAGAc > 2:446797/1‑139 (MQ=255) aaaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGt > 1:846272/1‑139 (MQ=255) aTCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatc > 1:350539/1‑139 (MQ=255) ccGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatcatc > 2:402800/1‑139 (MQ=255) tGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGt > 2:409223/1‑139 (MQ=255) tGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGt < 2:846272/139‑1 (MQ=255) gAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTg < 1:487147/139‑1 (MQ=255) aTGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTggg < 1:282718/139‑1 (MQ=255) tgtgGTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTggg < 2:869571/139‑1 (MQ=255) ggTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgtg < 1:409223/139‑1 (MQ=255) ggTCGGAAAACAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgtg < 2:851523/139‑1 (MQ=255) gTCGGAAAACAAAGGGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgtgt < 1:85568/139‑1 (MQ=255) gTCGGAAAACAAAGAGGAAA‑‑‑GGGGGG‑‑GGGT‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGa < 2:151180/81‑1 (MQ=255) gTCGGAAAACAAAGAGGAAA‑‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGa > 1:151180/1‑81 (MQ=255) aaaaaaaaGAGGAAAGG‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTa < 1:414457/139‑1 (MQ=255) aaaaCAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAc < 1:412206/139‑1 (MQ=255) aaCAAAGGGGAAG‑‑‑GGGGGGGTGGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAcc < 2:170357/139‑1 (MQ=255) aCAAAGAGGAAAG‑‑GGGGGG‑‑GGGG‑CTAA‑TAGGCAGGGAAGGCCGCCCCGGAAAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGATGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACcac > 2:470035/1‑139 (MQ=255) aaa‑‑‑GGGGGG‑‑GGGG‑CCAACCCGGAAGGGCAGGCCGCCCCGGATAGAGGGCGGCAGACGGATACAGATCTTCCAGGTCAGACGGGCTGCAAGTGTCAGACCGGTAAAATCATCGGTCGGGGTGTCGTACCACACTTTACCt > 2:570288/1‑139 (MQ=255) a‑‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGcc > 2:959089/1‑139 (MQ=255) | GGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGG‑‑GGGG‑CTAA‑TCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCC > NC_000913/379877‑380150 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |