Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,781,132	T→C	D532G (GAC→GGC)	ypjA ←	adhesin‑like autotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,781,132	0	T	C	100.0%	150.4 / NA	44	D532G (GAC→GGC)	ypjA	adhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/23);  total (21/23)

TGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGTCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCACTACCGTCAGTTCG  >  NC_000913/2781011‑2781263                                                                                                                           |                                                                                                                                    tGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAAcg                                                                                                                    >  1:866844/1‑139 (MQ=255)    aTTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAAcgccg                                                                                                                 <  1:830413/139‑1 (MQ=255)      tCCTGATGTTGAAAGTATCACCGTGACTGTTGGTTCCTTCGATAAGTGTTCCGTAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCTTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                               <  2:463513/139‑1 (MQ=255)       ccTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCa                                                                                                              <  2:503055/139‑1 (MQ=255)              tAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTaa                                                                                                       <  2:872501/139‑1 (MQ=255)                     cATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGt                                                                                                <  2:594594/139‑1 (MQ=255)                     cATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGt                                                                                                >  1:554050/1‑139 (MQ=255)                        cACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCt                                                                                             >  1:427263/1‑139 (MQ=255)                              aaCTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGt                                                                                       >  2:728062/1‑139 (MQ=255)                                    tGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTg                                                                                                                                 <  2:307324/91‑1 (MQ=255)                                    tGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTg                                                                                                                                 >  1:307324/1‑91 (MQ=255)                                     ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATc                                                                                          >  2:265858/1‑129 (MQ=255)                                     ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATc                                                                                          <  1:265858/129‑1 (MQ=255)                                     ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcc                                                                                <  1:674325/139‑1 (MQ=255)                                     ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcc                                                                                >  2:51348/1‑139 (MQ=255)                                     ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcc                                                                                <  1:826228/139‑1 (MQ=255)                                     ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcc                                                                                <  1:728062/139‑1 (MQ=255)                                           ttCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCa                                                                                                                         <  1:15315/92‑1 (MQ=255)                                           ttCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCa                                                                                                                         >  2:15315/1‑92 (MQ=255)                                           ttCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCatt                                                                                                            >  2:1030498/1‑105 (MQ=255)                                           ttCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCatt                                                                                                            <  1:1030498/105‑1 (MQ=255)                                             cGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAAcgccg                                                                                                                 >  1:740424/1‑98 (MQ=255)                                             cGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAAcgccg                                                                                                                 <  2:740424/98‑1 (MQ=255)                                                tAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGAc                                                                     <  1:834472/139‑1 (MQ=255)                                                    tgtTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATc                                                                 <  2:866844/139‑1 (MQ=255)                                                    tgtTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATc                                                                 >  2:892944/1‑139 (MQ=255)                                                      ttCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATc                                                                 >  2:146210/1‑137 (MQ=255)                                                      ttCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATc                                                                 <  1:146210/137‑1 (MQ=255)                                                        ccGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAAGACTCACCACCGCCCTGAACCTCCAGCACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGc                                                              >  2:589178/1‑139 (MQ=255)                                                         cGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACTTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCtt                                                            >  2:480002/1‑139 (MQ=255)                                                                cGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCa                                                     <  1:892944/139‑1 (MQ=255)                                                                  tGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGcc                                                      >  2:304720/1‑136 (MQ=255)                                                                  tGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGcc                                                      <  1:304720/136‑1 (MQ=255)                                                                      aGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATc                                                <  1:589178/139‑1 (MQ=255)                                                                       gCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCaa                                              <  1:178443/139‑1 (MQ=255)                                                                                              ttgCTCAACATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCATCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGtt                       >  2:635287/1‑139 (MQ=255)                                                                                                     aCATGCTT‑AGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCa                <  1:51348/139‑1 (MQ=255)                                                                                                               gCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCACTACCGTCa       >  2:461174/1‑139 (MQ=255)                                                                                                                    accaccGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTg                                                                                               >  2:1002039/1‑46 (MQ=255)                                                                                                                    accaccGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTg                                                                                               <  1:1002039/46‑1 (MQ=255)                                                                                                                    accaccGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGcc                                                      <  2:428193/87‑1 (MQ=255)                                                                                                                    accaccGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGcc                                                      >  1:428193/1‑87 (MQ=255)                                                                                                                    accaccGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCACTACCGTCAGTTCg  >  1:142116/1‑139 (MQ=255)                                                                                                                    accaccGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCACTACCGTCAGTTCg  >  1:101601/1‑139 (MQ=255)                                                                                                                           |                                                                                                                                    TGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTT‑AGCCTCACCACCGTCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCACTACCGTCAGTTCG  >  NC_000913/2781011‑2781263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑