Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,970,015	T→C	W329R (TGG→CGG)	wzzE →	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,970,015	0	T	C	100.0%	125.9 / NA	36	W329R (TGG→CGG)	wzzE	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/16);  total (20/16)

AATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTG  >  NC_000913/3969883‑3970148                                                                                                                                     |                                                                                                                                        aaTCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCa                                                                                                                                   >  1:914484/1‑139 (MQ=255)    cGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTg                                                                                                                                >  2:269087/1‑139 (MQ=255)      ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCgggg                                                                                                                                     <  2:456008/132‑1 (MQ=255)      ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCgggg                                                                                                                                     >  1:456008/1‑132 (MQ=255)      ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTc                                                                                                                              >  2:829781/1‑139 (MQ=255)                  cacCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGAt                                                                                                                 <  1:829781/139‑1 (MQ=255)                      cTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCggg                                                                                                             <  1:269087/139‑1 (MQ=255)                                   cAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCAt                                                                                                >  1:985486/1‑139 (MQ=255)                                     aCCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTa                                                                                              >  2:950544/1‑139 (MQ=255)                                     aCCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gg                                                                                                                           <  1:1034321/110‑1 (MQ=255)                                     aCCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gg                                                                                                                           >  2:1034321/1‑110 (MQ=255)                                         tGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTT‑gggg‑ggg                                                                                                                     >  2:625213/1‑110 (MQ=255)                                         tGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTT‑gggg‑ggg                                                                                                                     <  1:625213/111‑2 (MQ=255)                                           gATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAAcc                                                                                           >  2:9038/1‑136 (MQ=255)                                           gATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAAcc                                                                                           <  1:9038/136‑1 (MQ=255)                                                gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCgggg                                                                                                                                     <  1:221692/90‑1 (MQ=255)                                                gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCgggg                                                                                                                                     >  2:221692/1‑90 (MQ=255)                                                 cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc                                                                                  >  1:677444/1‑139 (MQ=255)                                                 cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gg                                                                                                                           <  2:596432/98‑1 (MQ=255)                                                 cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gg                                                                                                                           >  1:596432/1‑98 (MQ=255)                                                   ttttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc                                                                                <  1:950544/139‑1 (MQ=255)                                                              aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaacaac                                                                     >  1:347785/1‑139 (MQ=255)                                                              aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaacaac                                                                     >  2:818390/1‑139 (MQ=255)                                                               tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑GGGGTGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAcca                                                                    <  2:622192/139‑1 (MQ=255)                                                                      ttGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACtgctgc                                                             >  1:1069992/1‑139 (MQ=255)                                                                                          ggTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gggg                                                                                                                         <  1:61939/59‑1 (MQ=255)                                                                                          ggTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gggg                                                                                                                         >  2:61939/1‑59 (MQ=255)                                                                                               aaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGTTCGCCGCt                                    <  2:347785/139‑1 (MQ=255)                                                                                                     aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGgt                                                                                                       >  1:813607/1‑66 (MQ=255)                                                                                                     aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGgt                                                                                                       <  2:813607/66‑1 (MQ=255)                                                                                                                gtataagagacagtgatTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCGGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTGTTCGAAGAGAATCGa                   <  2:717768/126‑1 (MQ=255)                                                                                                                  tGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg                 >  1:1001059/1‑139 (MQ=255)                                                                                                                   gCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtgt                >  1:840274/1‑139 (MQ=255)                                                                                                                           gatgatTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTac        <  2:677444/139‑1 (MQ=255)                                                                                                                              gatTATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga     <  2:985486/139‑1 (MQ=255)                                                                                                                                 tATGCGGGGCATTGTCGGGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgactg  >  2:402956/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                        AATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGG‑GGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTG  >  NC_000913/3969883‑3970148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑