Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,447,891	G→A	*1260* (TAG→TAA)	tamB →	translocation and assembly module for autotransporter export, inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,447,891	0	G	A	100.0%	155.7 / NA	49	*1260* (TAG→TAA)	tamB	translocation and assembly module for autotransporter export, inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/24);  total (25/24)

TAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGC  >  NC_000913/4447788‑4448022                                                                                                        |                                                                                                                                    taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCcaca                                                                                                 >  1:277927/1‑139 (MQ=255)      acacTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCacaaacaa                                                                                            >  2:258121/1‑139 (MQ=255)         cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAatat                                                                                                                         >  2:265922/1‑107 (MQ=255)         cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAatat                                                                                                                         <  1:265922/107‑1 (MQ=255)         cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                         >  1:469772/1‑139 (MQ=255)         cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                         >  1:40013/1‑139 (MQ=255)                  cgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCAc                                                                                <  1:709680/139‑1 (MQ=255)                        cGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATg                                                                          >  1:354300/1‑139 (MQ=255)                            tGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                       >  2:910500/1‑122 (MQ=255)                            tGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                       <  1:910500/122‑1 (MQ=255)                             gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                                     <  2:40013/139‑1 (MQ=255)                                 ccTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                                                 <  2:206362/139‑1 (MQ=255)                                   tAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGcc                                                                                                    <  2:1026985/102‑1 (MQ=255)                                   tAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGcc                                                                                                    >  1:1026985/1‑102 (MQ=255)                                     aGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGt                                                             >  1:30113/1‑139 (MQ=255)                                          tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCAc                                                                                <  1:398197/115‑1 (MQ=255)                                          tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCAc                                                                                >  2:398197/1‑115 (MQ=255)                                           atCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACgg                                                                                                               <  2:52030/83‑1 (MQ=255)                                           atCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACgg                                                                                                               >  1:52030/1‑83 (MQ=255)                                           atCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTgg                                                       <  2:465531/139‑1 (MQ=255)                                                gAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAt                                                  >  1:85588/1‑139 (MQ=255)                                                gAAGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATAATTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAt                                                  >  1:214997/1‑139 (MQ=255)                                                 aaGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAcc                                                                       <  2:898938/117‑1 (MQ=255)                                                 aaGCCGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAcc                                                                       >  1:898938/1‑117 (MQ=255)                                                    ccGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAatat                                                                                                                         <  1:1045449/64‑1 (MQ=255)                                                    ccGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAatat                                                                                                                         >  2:1045449/1‑64 (MQ=255)                                                     cGTGTCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAg                                             >  2:75653/1‑139 (MQ=255)                                                      gtgtCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACACCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGt                                            <  1:1001433/139‑1 (MQ=255)                                                       tgtCTGGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTa                                           >  1:100026/1‑139 (MQ=255)                                                                tAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACt                                  <  1:395366/139‑1 (MQ=255)                                                                tAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACt                                  <  2:277927/139‑1 (MQ=255)                                                                  gACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                                <  2:354300/139‑1 (MQ=255)                                                                  gACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                                <  1:258121/139‑1 (MQ=255)                                                                   aCCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                       >  1:500401/1‑83 (MQ=255)                                                                   aCCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                       <  2:500401/83‑1 (MQ=255)                                                                     cAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg                             <  2:469772/139‑1 (MQ=255)                                                                        gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg                          >  2:247296/1‑139 (MQ=255)                                                                                  tGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTg                 <  2:30113/139‑1 (MQ=255)                                                                                       tATCAGTTCGAATTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCa            <  1:469067/139‑1 (MQ=255)                                                                                              tCGAGTTTTAACAATGCGTATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCa     >  1:706479/1‑139 (MQ=255)                                                                                              tCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg                             <  2:340271/115‑1 (MQ=255)                                                                                              tCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg                             >  1:340271/1‑115 (MQ=255)                                                                                              tCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACGGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCa     >  1:709638/1‑139 (MQ=255)                                                                                                gAGTTTTAACAATGCGAATAATTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAgg   <  2:214997/139‑1 (MQ=255)                                                                                                 aGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                       <  1:860589/54‑1 (MQ=255)                                                                                                 aGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                       >  2:860589/1‑54 (MQ=255)                                                                                                 aGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                       >  1:1065575/1‑54 (MQ=255)                                                                                                 aGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                       <  2:1065575/54‑1 (MQ=255)                                                                                                 aGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGCTTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGgc  >  1:205616/1‑139 (MQ=255)                                                                                                        |                                                                                                                                    TAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGC  >  NC_000913/4447788‑4448022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑