Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,821,867	(A)8→7	intergenic (‑248/+51)	chbB ← / ← osmE	N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,821,860	0	A	.	100.0%	276.2 / NA	64	intergenic (‑241/+58)	chbB/osmE	N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (32/32);  total (32/32)

AGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTA  >  NC_000913/1821745‑1821990                                                                                                                    |                                                                                                                                   aGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGt                                                                                                            >  1:222890/1‑139 (MQ=255)        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCaa                                                                                                                    >  2:773992/1‑124 (MQ=255)        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCaa                                                                                                                    <  1:773992/124‑1 (MQ=255)        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                         >  2:81114/1‑135 (MQ=255)        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                         <  1:81114/135‑1 (MQ=255)        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                     >  1:681000/1‑139 (MQ=255)         gCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgttg                                                                                                    <  2:448640/139‑1 (MQ=255)                  tttCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAg                                                                                           >  2:358112/1‑139 (MQ=255)                  tttCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAg                                                                                           >  2:423052/1‑139 (MQ=255)                  tttCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑‑AAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGc                                                                                          >  1:714126/1‑139 (MQ=255)                        tGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCa                                                                                     >  1:486868/1‑139 (MQ=255)                          aCCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGa                                                                                   >  2:1174347/1‑139 (MQ=255)                                   aTTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑‑AAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGAtt                                                                         <  2:714126/139‑1 (MQ=255)                                          ctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                   <  2:174109/139‑1 (MQ=255)                                          ctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                   <  1:423052/139‑1 (MQ=255)                                            ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                 <  2:131235/139‑1 (MQ=255)                                              ttGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGc                                                               <  2:754087/139‑1 (MQ=255)                                                      tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                       <  1:369019/139‑1 (MQ=255)                                                        gTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                         >  2:1050391/1‑87 (MQ=255)                                                        gTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                         <  1:1050391/87‑1 (MQ=255)                                                           aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                  >  2:600994/1‑139 (MQ=255)                                                            tGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtgt                                                 >  1:515492/1‑139 (MQ=255)                                                            tGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtgt                                                 >  2:767656/1‑139 (MQ=255)                                                             gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                <  2:222890/139‑1 (MQ=255)                                                             gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                <  1:767656/139‑1 (MQ=255)                                                             gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                <  2:515492/139‑1 (MQ=255)                                                             gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                >  1:89639/1‑139 (MQ=255)                                                                ccTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGaa                                                                               >  1:42045/1‑105 (MQ=255)                                                                ccTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGaa                                                                               <  2:42045/105‑1 (MQ=255)                                                                  tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAg                                                                                    >  1:157411/1‑98 (MQ=255)                                                                  tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAg                                                                                    <  2:157411/98‑1 (MQ=255)                                                                  tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                   <  2:335411/115‑1 (MQ=255)                                                                  tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                   >  1:335411/1‑115 (MQ=255)                                                                  tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                       >  2:1033284/1‑127 (MQ=255)                                                                  tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                       <  1:1033284/127‑1 (MQ=255)                                                                  tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTAtt                                           >  1:1227934/1‑139 (MQ=255)                                                                  tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTAtt                                           >  2:331456/1‑139 (MQ=255)                                                                   ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                   >  2:168072/1‑114 (MQ=255)                                                                   ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                   <  1:168072/114‑1 (MQ=255)                                                                   ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                  >  2:1092697/1‑131 (MQ=255)                                                                   ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                  <  1:1092697/131‑1 (MQ=255)                                                                          tttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgttg                                                                                                    <  1:849977/74‑1 (MQ=255)                                                                          tttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgttg                                                                                                    >  2:849977/1‑74 (MQ=255)                                                                          tttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                 >  2:502456/1‑109 (MQ=255)                                                                          tttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                 <  1:502456/109‑1 (MQ=255)                                                                            tCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGt                                 <  2:89639/139‑1 (MQ=255)                                                                             cGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTc                                >  1:1123771/1‑139 (MQ=255)                                                                              gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCt                               >  1:1322754/1‑139 (MQ=255)                                                                              gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCt                               >  2:1329346/1‑139 (MQ=255)                                                                                     taatTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAAcc                        <  1:331456/139‑1 (MQ=255)                                                                                       atTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCgg                      <  1:1174347/139‑1 (MQ=255)                                                                                                 aTAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc            <  1:1329346/139‑1 (MQ=255)                                                                                                 aTAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc            <  2:681000/139‑1 (MQ=255)                                                                                                  tAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACa           <  1:600994/139‑1 (MQ=255)                                                                                                          aaCTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGt   <  2:1227934/139‑1 (MQ=255)                                                                                                           aCTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGAtt                                                                         >  1:440000/1‑68 (MQ=255)                                                                                                           aCTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGAtt                                                                         <  2:440000/68‑1 (MQ=255)                                                                                                           aCTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTa  >  1:1183147/1‑139 (MQ=255)                                                                                                             tCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGa                                                                           >  2:1123068/1‑64 (MQ=255)                                                                                                             tCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGa                                                                           <  1:1123068/64‑1 (MQ=255)                                                                                                              cAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                 >  2:652653/1‑73 (MQ=255)                                                                                                              cAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                 <  1:652653/73‑1 (MQ=255)                                                                                                               aGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTg                              <  1:966065/107‑1 (MQ=255)                                                                                                               aGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTg                              >  2:966065/1‑107 (MQ=255)                                                                                                                    |                                                                                                                                   AGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTA  >  NC_000913/1821745‑1821990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑