| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,399,985 | (C)7→8 | coding (252/516 nt) | ogt ← | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,399,978 | 1 | . | C | 97.1% | 118.8 / ‑3.1 | 35 | coding (259/516 nt) | ogt | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (20/14); total (21/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.43e-01 | |||||||||||
ATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑CCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAT > NC_000913/1399844‑1400100 | aTTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccc‑c < 1:951669/139‑1 (MQ=255) ccGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGGAGGGGATAGTGCGTAGTGTTTTTCAGACTTCGCCCTGAAATGGCGT‑ccc‑cccccgta < 2:85555/139‑5 (MQ=255) tttGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCa > 1:566267/1‑139 (MQ=255) gCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGa < 2:566267/139‑1 (MQ=255) aCCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAg < 1:1049120/139‑1 (MQ=255) cgcgccgcgcCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCaata > 2:1090048/1‑139 (MQ=255) cgcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCt < 1:1090048/139‑1 (MQ=255) aTTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGt > 2:981112/1‑110 (MQ=255) aTTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGt < 1:981112/110‑1 (MQ=255) cAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCAc > 1:115516/1‑139 (MQ=255) ccGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGa < 2:419064/88‑1 (MQ=255) ccGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGa > 1:419064/1‑88 (MQ=255) cGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCaa > 1:549496/1‑97 (MQ=255) cGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCaa < 2:549496/97‑1 (MQ=255) gTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCt > 1:871474/1‑139 (MQ=255) gTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccc‑c > 1:755267/1‑70 (MQ=255) gTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccc‑c < 2:755267/70‑1 (MQ=255) gTAATGCATTACCTGCCCGAAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCt > 1:896361/1‑139 (MQ=255) aaTGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTa > 2:397320/1‑139 (MQ=255) aTGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAg < 2:731964/85‑1 (MQ=255) aTGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAg > 1:731964/1‑85 (MQ=255) tGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaaa < 2:115516/139‑1 (MQ=255) ccTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTg > 2:354267/1‑139 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAtt > 1:1274058/1‑139 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAtt > 1:634993/1‑139 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGCATCAATAATGCTAAGAtt < 1:885521/94‑1 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑CCCACCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGAtt > 2:885521/1‑94 (MQ=255) ccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATtg > 1:147377/1‑139 (MQ=255) aGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAAt > 1:1264482/1‑139 (MQ=255) tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGa < 1:528502/57‑1 (MQ=255) tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGa > 2:528502/1‑57 (MQ=255) tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cc < 1:152525/39‑1 (MQ=255) tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cc > 2:152525/1‑39 (MQ=255) gtgtTTTCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCAt < 2:871474/139‑1 (MQ=255) tCCAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCctt < 2:1264482/139‑1 (MQ=255) cAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCcttct < 2:147377/139‑1 (MQ=255) cAGACTTCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCcttct > 2:1112510/1‑139 (MQ=255) ttCGCGCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTACCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCg > 1:49846/1‑139 (MQ=255) cgcgCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGa < 2:700957/36‑1 (MQ=255) cgcgCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGa > 1:700957/1‑36 (MQ=255) cgcgCTGAAATGGCGTCCCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAt > 2:900245/1‑139 (MQ=255) | ATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑CCC‑CCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAT > NC_000913/1399844‑1400100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |