| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,897,050 | +G | coding (277/666 nt) | yieH → | phosphoenolpyruvate and 6‑phosphogluconate phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,897,046 | 1 | . | G | 96.6% | 203.5 / ‑0.7 | 59 | coding (273/666 nt) | yieH | phosphoenolpyruvate and 6‑phosphogluconate phosphatase |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (30/27); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑G‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGG > NC_000913/3896908‑3897177 | tttCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa < 1:436864/139‑1 (MQ=255) aGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑g‑gg‑ggc < 1:368772/139‑3 (MQ=255) aaaCTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAgcg < 1:133082/139‑1 (MQ=255) tACGAAATTATCGCTATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTg > 2:1170357/1‑139 (MQ=255) tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTg > 1:458727/1‑139 (MQ=255) tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTg < 2:273506/139‑1 (MQ=255) tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTg > 2:1230456/1‑139 (MQ=255) aTTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa < 1:1026441/114‑1 (MQ=255) aTTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa > 2:1026441/1‑114 (MQ=255) atTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCGATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTgcg < 1:820385/139‑1 (MQ=255) tCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtg < 2:200094/139‑1 (MQ=255) tGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAgc < 2:453488/106‑1 (MQ=255) tGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAgc > 1:453488/1‑106 (MQ=255) gAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtgtg > 1:884675/1‑139 (MQ=255) aaCATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa > 2:1229929/1‑92 (MQ=255) aaCATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa < 1:1229929/92‑1 (MQ=255) ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa > 1:800570/1‑87 (MQ=255) ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa < 2:800570/87‑1 (MQ=255) ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg > 2:654777/1‑131 (MQ=255) ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg > 2:582006/1‑131 (MQ=255) ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg < 1:582006/131‑1 (MQ=255) ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg < 1:654777/131‑1 (MQ=255) ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGt > 2:269456/1‑135 (MQ=255) ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGt < 1:269456/135‑1 (MQ=255) ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCt > 1:1337876/1‑139 (MQ=255) tACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACgg > 1:477159/1‑139 (MQ=255) aCGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGAt > 2:54181/1‑108 (MQ=255) aCGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGAt < 1:54181/108‑1 (MQ=255) aCGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGc < 2:458727/139‑1 (MQ=255) tAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCaa > 1:1158409/1‑139 (MQ=255) aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTc > 2:1282464/1‑92 (MQ=255) aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTc < 1:1282464/92‑1 (MQ=255) aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTg < 1:452714/95‑1 (MQ=255) aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTg > 2:452714/1‑95 (MQ=255) aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa < 1:570025/113‑1 (MQ=255) aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa > 2:570025/1‑113 (MQ=255) aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGACTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGcc < 1:724259/112‑1 (MQ=255) aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGACTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGcc > 2:724259/1‑112 (MQ=255) aaCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg > 1:1005089/1‑114 (MQ=255) aaCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg < 2:1005089/114‑1 (MQ=255) aGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAAt > 1:442630/1‑139 (MQ=255) aaGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgc > 1:563727/1‑139 (MQ=255) gCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcag < 2:367144/139‑1 (MQ=255) aaCACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCAtt > 2:338714/1‑139 (MQ=255) tACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAAGTGGAAGCCATCGAA‑G‑GGAGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc < 2:884675/139‑1 (MQ=255) tACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc < 1:1230456/139‑1 (MQ=255) tACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc < 2:1337876/139‑1 (MQ=255) ccGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑GTGG‑GCTGGAGCGCTCATGTCAGCGATCACTGCGCCAATGTGTGTGTTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATTGGCaa < 2:477159/139‑1 (MQ=255) tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgt > 2:544431/1‑87 (MQ=255) tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgt < 1:544431/87‑1 (MQ=255) tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCt > 1:417765/1‑139 (MQ=255) aaGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAata < 2:1158409/139‑1 (MQ=255) gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTg > 1:1247234/1‑139 (MQ=255) tCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATg < 2:526731/107‑1 (MQ=255) tCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATg > 1:526731/1‑107 (MQ=255) ttCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTg < 1:1223397/46‑1 (MQ=255) ttCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTg > 2:1223397/1‑46 (MQ=39) ttCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTc > 2:1158231/1‑139 (MQ=255) cAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATa > 2:48243/1‑139 (MQ=255) gAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTgcg < 1:994558/46‑1 (MQ=255) gAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcag < 2:1043391/88‑1 (MQ=255) gAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcag > 1:1043391/1‑88 (MQ=255) gAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTc > 2:228569/1‑139 (MQ=255) gAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGCCAGCGATCACTgcg > 2:994558/1‑46 (MQ=255) gCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGc < 1:48243/139‑1 (MQ=255) cATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg < 2:442630/139‑1 (MQ=255) cATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCga < 1:228569/139‑2 (MQ=255) | TTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑G‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGG > NC_000913/3896908‑3897177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |