Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,906,662	(A)5→4	coding (399/810 nt)	rlmA ←	23S rRNA m(1)G745 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,906,658	0	A	.	100.0%	250.9 / NA	60	coding (403/810 nt)	rlmA	23S rRNA m(1)G745 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (29/31);  total (29/31)

GCCGCCGGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATC  >  NC_000913/1906545‑1906790                                                                                                                  |                                                                                                                                     gccgccGGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTcg                                                                                                            >  1:178615/1‑139 (MQ=255)          cTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCtt                                                                                                   <  2:154908/139‑1 (MQ=255)                  ctCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCt                                                                                           <  2:718139/139‑1 (MQ=255)                       gCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCtt                                                                                      >  1:990132/1‑139 (MQ=255)                       gCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCtt                                                                                      >  2:1718190/1‑139 (MQ=255)                       gCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCtt                                                                                      >  1:210122/1‑139 (MQ=255)                       gCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGAGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCtt                                                                                      >  1:335912/1‑139 (MQ=255)                                tctGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATc                                                                             <  2:614593/139‑1 (MQ=255)                                      ttACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACACaaaagt                                                                                                                                 >  2:1631503/1‑80 (MQ=255)                                      ttACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACACaaaagt                                                                                                                                 <  1:1631503/82‑3 (MQ=255)                                       tACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCa                                                                      >  1:666981/1‑139 (MQ=255)                                         cacGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCaaa                                                                    <  2:210122/139‑1 (MQ=255)                                              cgcgTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAATTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATc                                                                             >  1:333042/1‑125 (MQ=255)                                              cgcgTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTcgccgc                                                                                                        <  1:556616/98‑1 (MQ=255)                                              cgcgTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTcgccgc                                                                                                        >  2:556616/1‑98 (MQ=255)                                              cgcgTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATc                                                                             <  2:333042/125‑1 (MQ=255)                                                   aaaTACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCt                                                                                           <  1:942034/106‑1 (MQ=255)                                                   aaaTACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCt                                                                                           >  2:942034/1‑106 (MQ=255)                                                            ttatGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGAttt                                                         <  1:30907/131‑1 (MQ=255)                                                            ttatGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGAttt                                                         >  2:30907/1‑131 (MQ=255)                                                             tatGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACg                                                >  1:1792879/1‑139 (MQ=255)                                                             tatGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACg                                                >  2:1418969/1‑139 (MQ=255)                                                              atGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGc                                               <  1:39974/139‑1 (MQ=255)                                                              atGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGc                                               >  2:83930/1‑139 (MQ=255)                                                                  cGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCttt                                                                                                  >  1:1162177/1‑84 (MQ=255)                                                                  cGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCttt                                                                                                  <  2:1162177/84‑1 (MQ=255)                                                                  cGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCg                                           >  2:1023634/1‑139 (MQ=255)                                                                   gTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTAcc                                                                                        >  2:1760037/1‑93 (MQ=255)                                                                   gTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTAcc                                                                                        <  1:1760037/93‑1 (MQ=255)                                                                    tCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACgtgg                                                               >  1:1018819/1‑117 (MQ=255)                                                                    tCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACgtgg                                                               <  2:1018819/117‑1 (MQ=255)                                                                       aTACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCaaa                                      <  1:83930/139‑1 (MQ=255)                                                                       aTACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCaaa                                      <  2:178615/139‑1 (MQ=255)                                                                          cTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGc                                   <  1:700866/139‑1 (MQ=255)                                                                            ggTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCgt                                 <  2:72979/139‑1 (MQ=255)                                                                             gTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCgtg                                >  2:1834591/1‑139 (MQ=255)                                                                               aTCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAgtc                                                                        <  1:1099551/97‑3 (MQ=255)                                                                               aTCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGAc                                                                        >  2:1099551/1‑97 (MQ=255)                                                                               aTCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCa                                                   >  2:733465/1‑118 (MQ=255)                                                                               aTCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCa                                                   <  1:733465/118‑1 (MQ=255)                                                                               aTCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCgtgtg                              >  1:1125043/1‑139 (MQ=255)                                                                                tCGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCgtgtgt                             >  1:1365975/1‑139 (MQ=255)                                                                                 cGGAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCgtgtgtg                            <  2:666981/139‑1 (MQ=255)                                                                                  ggAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGa                                                                                   >  2:795670/1‑83 (MQ=255)                                                                                  ggAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGa                                                                                   <  1:795670/83‑1 (MQ=255)                                                                                  ggAAAACGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATCTCGGGCAACGCATCGGCAAATGCgtgtgtgt                           >  2:932702/1‑139 (MQ=255)                                                                                    aaaaCGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTaa                         <  2:335912/139‑1 (MQ=255)                                                                                     aaaCGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGc                                   >  2:815120/1‑128 (MQ=255)                                                                                     aaaCGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGc                                   <  1:815120/128‑1 (MQ=255)                                                                                       aCGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGaa                                                                                  <  1:1566384/79‑1 (MQ=255)                                                                                       aCGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGaa                                                                                  >  2:992537/1‑79 (MQ=255)                                                                                       aCGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGaa                                                                                  >  2:1566384/1‑79 (MQ=255)                                                                                       aCGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGaa                                                                                  <  1:992537/79‑1 (MQ=255)                                                                                       aCGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGa                                                                         >  2:491665/1‑88 (MQ=255)                                                                                       aCGGCAAACGGTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGa                                                                         <  1:491665/88‑1 (MQ=255)                                                                                                ggTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTcacca             <  1:1023634/139‑1 (MQ=255)                                                                                                 gTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTcaccac            <  2:1792879/139‑1 (MQ=255)                                                                                                 gTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTcaccac            <  2:1125043/139‑1 (MQ=255)                                                                                                 gTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTcaccac            <  2:990132/139‑1 (MQ=255)                                                                                                 gTGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTcaccac            <  1:1718190/139‑1 (MQ=255)                                                                                                  tGGCTGGAAGCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACcaca           >  2:545691/1‑139 (MQ=255)                                                                                                           gCGACAC‑AAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATc  <  2:1365975/139‑1 (MQ=255)                                                                                                                  |                                                                                                                                     GCCGCCGGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATC  >  NC_000913/1906545‑1906790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑