| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,484,897 | (C)6→7 | coding (524/3594 nt) | evgS → | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,484,891 | 1 | . | C | 98.3% | 209.4 / ‑3.4 | 60 | coding (518/3594 nt) | evgS | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (30/29); total (31/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.81e-01 | |||||||||||
TTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAG > NC_000913/2484754‑2485023 | ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑c < 2:194571/139‑1 (MQ=255) tctTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑cccccccaga < 1:1069094/139‑5 (MQ=255) cATTGCTGCAACCACACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑c < 2:1726952/118‑1 (MQ=255) cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑c > 1:1726952/1‑118 (MQ=255) tgctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑cccccccagac > 1:1343976/1‑120 (MQ=255) tgctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑cccccccagac < 2:1343976/125‑6 (MQ=255) ctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCatcaat > 1:585212/1‑139 (MQ=255) cAACCAAACCATTGATAATTACCTTTCCGGTGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCatcaatc > 1:447039/1‑137 (MQ=255) cAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAGCCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCatcaatc < 2:447039/137‑1 (MQ=255) ccaaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTc > 2:104367/1‑139 (MQ=255) aaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCa < 1:1329579/139‑1 (MQ=255) accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCatcaatc > 2:425100/1‑130 (MQ=255) accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCatcaatc < 1:425100/130‑1 (MQ=255) tGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑cccccccagac > 2:986126/1‑103 (MQ=255) tGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑cccccccagac < 1:986126/108‑6 (MQ=255) ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGc < 2:117044/125‑1 (MQ=255) ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGc > 1:117044/1‑125 (MQ=255) ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCtt > 1:555261/1‑139 (MQ=255) tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCttt > 1:1414444/1‑139 (MQ=255) ataagaaacaaaaCCACCCTTCACAACTCAATGCGACCCCTTACCTCACCAAAACCAGAAAATTTTGCTCGGTAAGCAAATTAC‑CCCCCC‑GGACAAGTTAATTCATC‑ATCATTTCCAAAAGCAACAATTATCTCTTTTa < 2:1325722/128‑1 (MQ=255) cGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa < 1:223397/139‑1 (MQ=255) ggCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTAc < 1:1684711/139‑1 (MQ=255) ccaccCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATc > 2:106427/1‑139 (MQ=255) ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGc < 2:555261/139‑1 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt > 2:5921/1‑139 (MQ=255) tCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAtt < 1:5921/139‑1 (MQ=255) tCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAtt > 1:719451/1‑139 (MQ=255) aCGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATC‑ATCCTTTCCAAAAGCAAAAATTAACTCTTTTAAAAATTtataacctttttctat > 1:1325722/1‑127 (MQ=255) aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGcaac < 2:1216909/97‑1 (MQ=255) aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGcaac > 1:1216909/1‑97 (MQ=255) aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGcaa > 2:586014/1‑96 (MQ=255) aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGcaa < 1:586014/96‑1 (MQ=255) tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTaa < 2:1472222/70‑1 (MQ=255) tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTaa > 1:1472222/1‑70 (MQ=255) tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTAc > 1:394513/1‑110 (MQ=255) tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTAc < 2:394513/110‑1 (MQ=255) aCCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGc < 2:585212/139‑1 (MQ=255) ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCt > 2:1613217/1‑139 (MQ=255) ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCt > 1:1426142/1‑139 (MQ=255) ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCt > 1:1382052/1‑139 (MQ=255) ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCt > 1:1003216/1‑139 (MQ=255) cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATtcat < 1:1469067/67‑1 (MQ=255) cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATtcat > 2:1469067/1‑67 (MQ=255) ttACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc < 2:719451/139‑1 (MQ=255) tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑cccccccaga > 1:28305/1‑48 (MQ=255) tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑cccccccaga < 2:28305/52‑5 (MQ=255) aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACAAGGAAATTCATCAATAATTTCAAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGAATTAGCATCCGTCTCAGCTGGCCAAAATGa > 2:747549/1‑139 (MQ=255) ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt < 2:1382052/139‑1 (MQ=255) cAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAtt < 2:1414444/139‑1 (MQ=255) aaCCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat > 1:1385105/1‑99 (MQ=255) aaCCAGTAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat < 2:1385105/99‑1 (MQ=255) tAAATATTGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTg < 1:106427/139‑1 (MQ=255) ttGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagta > 2:274445/1‑139 (MQ=255) ttGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagta < 1:747549/139‑1 (MQ=255) tGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCa > 2:22753/1‑53 (MQ=255) tGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCa < 1:22753/53‑1 (MQ=255) tGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAAtt > 1:1117022/1‑81 (MQ=255) tGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAAtt < 2:1117022/81‑1 (MQ=255) tGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTtata > 2:247948/1‑85 (MQ=255) tGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTtata < 1:247948/85‑1 (MQ=255) tGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt < 2:381161/122‑1 (MQ=255) tGCTCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt > 1:381161/1‑122 (MQ=255) tGCTCGGGTAGCAAATTA‑‑CCCCCCAAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCTTTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGTTAGTaa > 2:1608851/1‑139 (MQ=255) tCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt < 2:948110/90‑1 (MQ=255) tCGGGTAGCAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt > 1:948110/1‑90 (MQ=255) gCAAATTACCCCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca > 1:355549/1‑132 (MQ=255) gCAAATTACCCCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca < 2:355549/132‑1 (MQ=255) cAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa < 2:1071776/64‑1 (MQ=255) cAAATTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa > 1:1071776/1‑64 (MQ=255) aaaTTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACcag < 2:1003216/139‑1 (MQ=255) aaaTTAC‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACcag < 2:1426142/139‑1 (MQ=255) | TTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑CCCCCC‑AGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAG > NC_000913/2484754‑2485023 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |