Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	675,570	C→T	*326* (TAG→TAA)	ybeQ ←	Sel1 family TPR‑like repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	675,570	0	C	T	100.0%	178.7 / NA	56	*326* (TAG→TAA)	ybeQ	Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/30);  total (26/30)

GAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGG  >  NC_000913/675435‑675705                                                                                                                                        |                                                                                                                                        gAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTAc                                                                                                                                      >  1:907678/1‑139 (MQ=255) gAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTAc                                                                                                                                      >  2:816233/1‑139 (MQ=255)           cGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCtt                                                                                                                            <  2:1668583/139‑1 (MQ=255)                      aaaTGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGa                                                                                                                 >  1:86277/1‑139 (MQ=255)                              tCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTccga                                                                                                         <  2:32292/139‑3 (MQ=255)                                aTGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTccgact                                                                                                       <  2:1459953/139‑5 (MQ=255)                                   aCCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCc‑gactgac                                                                                                   <  1:1618607/139‑1 (MQ=255)                                       gTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACAtct                                                                                               <  2:86277/139‑1 (MQ=255)                                       gTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACAtct                                                                                               >  2:680131/1‑139 (MQ=255)                                        ttCCAGAGACGCCCGTTTGAGTCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACAtctc                                                                                              >  1:538556/1‑139 (MQ=255)                                        ttCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACAtctc                                                                                              <  2:684181/139‑1 (MQ=255)                                            agagACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGt                                                                                          >  1:1527623/1‑139 (MQ=255)                                               gaCGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTcc                                                                                       >  1:666769/1‑139 (MQ=255)                                                  gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGa                                                                                    <  2:1527623/139‑1 (MQ=255)                                                     cGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGa                                                                                 <  2:666769/139‑1 (MQ=255)                                                       tttGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACt                                                                               >  2:1802552/1‑139 (MQ=255)                                                        ttGAGCCGTTATAGTCTGATTTCGCACAGCAATACAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTg                                                                              <  2:1174497/139‑1 (MQ=255)                                                        ttGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTg                                                                              >  1:134406/1‑139 (MQ=255)                                                          gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGaa                                                                            <  2:1455893/139‑1 (MQ=255)                                                          gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGaa                                                                            <  2:84705/139‑1 (MQ=255)                                                                    aGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATa                                                                  <  2:538556/139‑1 (MQ=255)                                                                      tCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACa                                                                <  2:134406/139‑1 (MQ=255)                                                                          aTTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTc                                                            >  1:1582345/1‑139 (MQ=255)                                                                                    gCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTccga                                                                                                         >  2:453180/1‑83 (MQ=255)                                                                                    gCAATAAAGAGAATATGGGCCGAAAGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTccga                                                                                                         <  1:453180/85‑3 (MQ=255)                                                                                             agaATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGaa                                                                            >  2:364967/1‑104 (MQ=255)                                                                                             agaATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGaa                                                                            <  1:364967/104‑1 (MQ=255)                                                                                             agaATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCg                                         >  2:1809724/1‑139 (MQ=255)                                                                                               aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTc                                                                                                                           <  2:659360/56‑1 (MQ=255)                                                                                               aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTc                                                                                                                           >  1:659360/1‑56 (MQ=255)                                                                                               aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCg                                                                                      >  2:17160/1‑92 (MQ=255)                                                                                               aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCg                                                                                      <  1:17160/92‑1 (MQ=255)                                                                                                atatGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACa                                      <  2:1582345/139‑1 (MQ=255)                                                                                                atatGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACa                                      <  1:1802552/139‑1 (MQ=255)                                                                                                  atGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATc                                          >  2:1534195/1‑133 (MQ=255)                                                                                                  atGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATc                                          <  1:1534195/133‑1 (MQ=255)                                                                                                    gggCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCc‑gactgac                                                                                                   <  1:1247815/74‑1 (MQ=255)                                                                                                    gggCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCc‑gactgac                                                                                                   >  2:1247815/1‑74 (MQ=255)                                                                                                      gCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAgg                                    <  2:911280/135‑1 (MQ=255)                                                                                                      gCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAgg                                    >  1:911280/1‑135 (MQ=255)                                                                                                       ccGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTccga                                                                                                         <  2:1635508/66‑3 (MQ=255)                                                                                                       ccGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTccga                                                                                                         >  1:1635508/1‑64 (MQ=255)                                                                                                         gAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttc                               >  2:1522501/1‑137 (MQ=255)                                                                                                         gAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttc                               <  1:1522501/137‑1 (MQ=255)                                                                                                          aaTGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACAtctc                                                                                              <  1:1046560/73‑1 (MQ=255)                                                                                                          aaTGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACAtctc                                                                                              >  2:1046560/1‑73 (MQ=255)                                                                                                          aaTGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttcctt                            >  2:551886/1‑139 (MQ=255)                                                                                                               aaTTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCg                       <  2:1547754/139‑1 (MQ=255)                                                                                                                 ttCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTa                     <  1:551886/139‑1 (MQ=255)                                                                                                                      cGGCCCTTGTTTTTATCTTTACAGTAAATCTCCAGTCTCACGAATTT‑CAGACTGACATCTCTGGTTCTGGATGACTGAACTCTACGAGACAGGTCGGGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTAAt                >  1:773408/1‑139 (MQ=255)                                                                                                                           cTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACa                                                                >  1:918781/1‑86 (MQ=255)                                                                                                                           cTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACa                                                                <  2:918781/86‑1 (MQ=255)                                                                                                                             tGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCg                       >  1:295295/1‑125 (MQ=255)                                                                                                                             tGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCg                       <  2:295295/125‑1 (MQ=255)                                                                                                                              gTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATg        >  2:186683/1‑139 (MQ=255)                                                                                                                                    aTCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAgg  <  1:680131/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        GAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGG  >  NC_000913/675435‑675705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑