Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	677,889	G→A	A159T (GCC→ACC)	djlB →	putative HscC co‑chaperone, uncharacterized J domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	677,889	0	G	A	100.0%	159.8 / NA	50	A159T (GCC→ACC)	djlB	putative HscC co‑chaperone, uncharacterized J domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/26);  total (24/26)

ATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTG  >  NC_000913/677766‑678014                                                                                                                            |                                                                                                                               aTCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGa                                                                                                                <  2:996762/139‑1 (MQ=255)               tGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGttttt                                                                                                        >  2:1483269/1‑133 (MQ=255)               tGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGttttt                                                                                                        <  1:1483269/133‑1 (MQ=255)               tGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTc                                                                                                  >  2:463613/1‑139 (MQ=255)                   cATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGa                                                                                              <  1:463613/139‑1 (MQ=255)                    aTTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGAc                                                                                             >  1:844782/1‑139 (MQ=255)                         tCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGAc                                                                                             <  1:1326508/134‑1 (MQ=255)                         tCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGAc                                                                                             >  2:1326508/1‑134 (MQ=255)                              cGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCAt                                                                                   <  2:1803743/139‑1 (MQ=255)                                aGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACTGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCt                                                                                 >  1:46778/1‑139 (MQ=255)                                aGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCt                                                                                 >  2:704911/1‑139 (MQ=255)                                    cGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGttt                                                                                                          >  1:1235985/1‑110 (MQ=255)                                    cGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGttt                                                                                                          <  2:1235985/110‑1 (MQ=255)                                    cGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCAtt                                                                                         >  1:1112312/1‑127 (MQ=255)                                    cGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCAtt                                                                                         <  2:465842/127‑1 (MQ=255)                                    cGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCAtt                                                                                         <  2:1112312/127‑1 (MQ=255)                                    cGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCAtt                                                                                         >  1:465842/1‑127 (MQ=255)                                    cGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGccc                                                                             >  1:1472683/1‑139 (MQ=255)                                     gtggtgTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCg                                                                            <  2:1472683/139‑1 (MQ=255)                                        gtgtTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTg                                                                         <  1:303313/139‑1 (MQ=255)                                         tgtTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGc                                                                        <  2:759065/139‑1 (MQ=255)                                           ttattaGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCg                                                                      >  2:1059683/1‑139 (MQ=255)                                                   aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAGCTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAAt                                                              >  1:1157220/1‑139 (MQ=255)                                                     aaaGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGCAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑ggggc                                                                                                                <  2:1126522/87‑2 (MQ=255)                                                     aaaGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATg                                                             >  2:391305/1‑139 (MQ=255)                                                     aaaGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGa                                                                                                                >  1:1126522/1‑87 (MQ=255)                                                     aaaGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCg                                                                            >  2:830121/1‑123 (MQ=255)                                                     aaaGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCg                                                                            <  1:830121/123‑1 (MQ=255)                                                     aaaGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGa                                                            >  2:140274/1‑139 (MQ=255)                                                           tGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCa                                                                  <  2:243114/127‑1 (MQ=255)                                                           tGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCa                                                                  >  1:243114/1‑127 (MQ=255)                                                                  gtTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGAtttt                                               >  2:623591/1‑139 (MQ=255)                                                                      cAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTAcc                                           <  2:844782/139‑1 (MQ=255)                                                                            gaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGt                                     >  2:286425/1‑139 (MQ=255)                                                                            gaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGt                                     >  1:331402/1‑139 (MQ=255)                                                                                 tACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTa                                                                                                       <  2:923920/68‑1 (MQ=255)                                                                                 tACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTa                                                                                                       >  1:923920/1‑68 (MQ=255)                                                                                 tACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCt                                <  1:888032/139‑1 (MQ=255)                                                                                 tACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCt                                >  2:888032/1‑139 (MQ=255)                                                                                          aaTAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTgg                       <  2:46778/139‑1 (MQ=255)                                                                                            tAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTc                     <  2:331402/139‑1 (MQ=255)                                                                                            tAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGACTTTTACCAAATGTTCGCTCGGATTTGGTc                     <  1:1059683/139‑1 (MQ=255)                                                                                              gACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCAt                   <  1:1436004/139‑1 (MQ=255)                                                                                                   agaagaaCTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCt                                <  2:411880/121‑1 (MQ=255)                                                                                                   agaagaaCTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCt                                >  1:411880/1‑121 (MQ=255)                                                                                                      agaaCTGGAATCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCca           <  2:338745/139‑1 (MQ=255)                                                                                                               aTCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTg  <  1:1285261/139‑1 (MQ=255)                                                                                                               aTCCTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTg  <  2:1157220/139‑1 (MQ=255)                                                                                                                  cTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCAt                                                      >  2:320606/1‑84 (MQ=255)                                                                                                                  cTTTTTATATACCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCAt                                                      <  1:320606/84‑1 (MQ=255)                                                                                                                            |                                                                                                                               ATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAA‑GGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTG  >  NC_000913/677766‑678014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑