Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,565,464	C→A	L129F (TTG→TTT)	yjiM ←	putative 2‑hydroxyglutaryl‑CoA dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,565,464	0	C	A	100.0%	206.2 / NA	64	L129F (TTG→TTT)	yjiM	putative 2‑hydroxyglutaryl‑CoA dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/36);  total (28/36)

TTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGT  >  NC_000913/4565332‑4565597                                                                                                                                      |                                                                                                                                      tttCAGCGCAATGGCATCGCGAAGAGCATCTTCGCTAA‑CTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCa                                                                                                                                <  2:924253/139‑1 (MQ=255)  ttCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCa                                                                                                                                >  1:941961/1‑139 (MQ=255)      gcgcAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATcac                                                                                                                            <  2:941961/139‑1 (MQ=255)           aTGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGaa                                                                                                                       <  1:1412985/139‑1 (MQ=255)            tGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGGTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAAc                                                                                                                      >  1:1029380/1‑139 (MQ=255)                    cgcAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTaa                                                                                                              >  1:673029/1‑139 (MQ=255)                    cgcAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTaa                                                                                                              >  1:641845/1‑139 (MQ=255)                    cgcAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTaa                                                                                                              >  1:1603822/1‑139 (MQ=255)                       agagCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACt                                                                                                           <  2:394878/139‑1 (MQ=255)                           cATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGc                                                                                                       <  2:234236/139‑1 (MQ=255)                            aTCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCACAGCATCTCGGCTTT‑CCATAGCGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGcc                                                                                                      <  2:1174459/139‑1 (MQ=255)                              cTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCAt                                                                                                    <  2:673029/139‑1 (MQ=255)                              cTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCAt                                                                                                    <  1:427467/139‑1 (MQ=255)                               ttCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATg                                                                                                   <  2:641845/139‑1 (MQ=255)                               ttCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATg                                                                                                   <  2:1603822/139‑1 (MQ=255)                                       tctcGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGGTGTTGGGAAATTGCATCACATGaa                                                                                                                       <  1:1240611/111‑1 (MQ=255)                                       tctcGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCCTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATgga                                                                                                                       >  2:1240611/1‑109 (MQ=255)                                           gTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGaa                                                                                                                       >  2:320842/1‑107 (MQ=255)                                           gTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGaa                                                                                                                       <  1:320842/107‑1 (MQ=255)                                            tGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAAt                                                                                                                                    <  1:833347/93‑1 (MQ=255)                                            tGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCATTAACGCTGTTGGGAAAt                                                                                                                                    >  2:833347/1‑93 (MQ=255)                                             gCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACAtttt                                                                                     >  1:1628752/1‑139 (MQ=255)                                              cccAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGcc                                                                                                      <  1:559193/121‑1 (MQ=255)                                              cccAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGcc                                                                                                      >  2:559193/1‑121 (MQ=255)                                                cAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCtt                                                                                  >  2:1000609/1‑139 (MQ=255)                                                   aaCGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTaaa                                                                                                             >  1:1178200/1‑110 (MQ=255)                                                   aaCGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTaaa                                                                                                             <  2:1178200/110‑1 (MQ=255)                                                         cttctACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTc                                                                         <  2:1468926/139‑1 (MQ=255)                                                                            gcgcAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATcac                                                                                                                            <  2:1301484/69‑1 (MQ=255)                                                                            gcgcAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATcac                                                                                                                            >  1:1301484/1‑69 (MQ=255)                                                                              gcagcaTCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGaccac                                                    <  2:1029380/139‑1 (MQ=255)                                                                                      tcGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCg                                            <  2:1382696/139‑1 (MQ=255)                                                                                      tcGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCg                                            <  1:770797/139‑1 (MQ=255)                                                                                      tcGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCg                                            <  2:1476366/139‑1 (MQ=255)                                                                                        ggCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGaa                                                                                                                       >  1:750604/1‑62 (MQ=255)                                                                                        ggCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGaa                                                                                                                       <  2:750604/62‑1 (MQ=255)                                                                                                cATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCg                                                         >  2:1538981/1‑117 (MQ=255)                                                                                                cATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCg                                                         <  1:1538981/117‑1 (MQ=255)                                                                                                       cacgcGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCa                                                                      <  2:1781500/97‑1 (MQ=255)                                                                                                       cacgcGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCa                                                                      >  1:1781500/1‑97 (MQ=255)                                                                                                       cacgcGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCg                                                         >  1:69495/1‑110 (MQ=255)                                                                                                       cacgcGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCg                                                         <  2:69495/110‑1 (MQ=255)                                                                                                        acgcGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAgggg                           >  1:460937/1‑139 (MQ=255)                                                                                                        acgcGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAgggg                           >  2:1496267/1‑139 (MQ=255)                                                                                                        acgcGAGGCATCGTCCTTAACGCTGTTCGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAgggg                           <  1:1047487/139‑1 (MQ=255)                                                                                                         cgcgAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCa                                                                                                     <  1:1269834/64‑1 (MQ=255)                                                                                                         cgcgAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCa                                                                                                     >  2:1269834/1‑64 (MQ=255)                                                                                                         cgcgAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCg                                                         <  1:516843/108‑1 (MQ=255)                                                                                                         cgcgAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCg                                                         >  2:516843/1‑108 (MQ=255)                                                                                                          gcgAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCa                                                                                             >  2:1345148/1‑71 (MQ=255)                                                                                                          gcgAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCa                                                                                             <  1:1345148/71‑1 (MQ=255)                                                                                                          gcgAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGtt                                                               <  1:1615394/101‑1 (MQ=255)                                                                                                          gcgAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGtt                                                               >  2:1615394/1‑101 (MQ=255)                                                                                                          gcgAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCa                         <  2:406421/139‑1 (MQ=255)                                                                                                              ggCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTa                     <  2:1564290/139‑1 (MQ=255)                                                                                                                    gTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGtt               <  1:440489/139‑1 (MQ=255)                                                                                                                          aaCGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATg                                                                                                   <  1:58014/49‑1 (MQ=255)                                                                                                                          aaCGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATg                                                                                                   >  2:58014/1‑49 (MQ=255)                                                                                                                            cGCTGTTGGGAAATTGCGTCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCg                                                         <  1:1052390/89‑1 (MQ=255)                                                                                                                            cGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCg                                                         >  2:1052390/1‑89 (MQ=255)                                                                                                                            cGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGaa       >  2:868059/1‑139 (MQ=255)                                                                                                                                gTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAggt                                                                   >  2:1255846/1‑75 (MQ=255)                                                                                                                                gTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAggt                                                                   <  1:1255846/75‑1 (MQ=255)                                                                                                                                 ttGGGAAATTGCATCACATGAACAGGCTTAAACTTCGCCATGTATTCATACATTTTCTTTTTGACGTCGCAGGTGGTTTCACCCACCAACAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGACGAAGCCgc  >  1:170366/1‑138 (MQ=255)                                                                                                                                      |                                                                                                                                      TTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTT‑CCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGT  >  NC_000913/4565332‑4565597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑