Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,029,729	(C)6→5	coding (191/921 nt)	yedA →	amino acid exporter for phenylalanine, threonine

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,029,724	0	C	.	100.0%	190.5 / NA	46	coding (186/921 nt)	yedA	amino acid exporter for phenylalanine, threonine
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (22/24);  total (22/24)

GGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGCC  >  NC_000913/2029597‑2029858                                                                                                                                |                                                                                                                                       ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTcccccgct                                                                                                                                 <  2:767972/135‑4 (MQ=255) ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTcccccgct                                                                                                                                 >  1:767972/1‑132 (MQ=255) ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGt                                                                                                                            >  1:1184724/1‑139 (MQ=255) ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGt                                                                                                                            >  2:742452/1‑139 (MQ=255) ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGt                                                                                                                            >  2:491113/1‑139 (MQ=255) ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGt                                                                                                                            >  1:917318/1‑139 (MQ=255) ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTA‑TGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTc                                                                                                                           >  2:1169535/1‑139 (MQ=255)    gCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCg                                                                                                                         <  1:909034/139‑1 (MQ=255)       cAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCgctg                                                                                                                      >  2:71988/1‑139 (MQ=255)         aCCTATTTTGTCCTTCGGATTGGCGGGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCgctggt                                                                                                                    <  1:1152686/139‑3 (MQ=255)                                         cTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctgg                                                                                    <  2:950853/139‑1 (MQ=255)                                         cTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctgg                                                                                    >  1:487229/1‑139 (MQ=255)                                             ccTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATCGGCCTGTTATTGCTGGCTGt                                                                                <  2:928073/139‑1 (MQ=255)                                                   ttAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTaa                                                                          <  2:1184724/139‑1 (MQ=255)                                                           ggcgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGAt                                                                                                     <  2:882150/104‑1 (MQ=255)                                                           ggcgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGAt                                                                                                     >  1:882150/1‑104 (MQ=255)                                                           ggcgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGc                                                                                                 >  1:31986/1‑108 (MQ=255)                                                           ggcgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGc                                                                                                 <  2:31986/108‑1 (MQ=255)                                                            gcgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGt                                                                 >  2:1371964/1‑139 (MQ=255)                                                             cgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTg                                                                >  1:15643/1‑139 (MQ=255)                                                               ggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGAc                                                              <  2:15643/139‑1 (MQ=255)                                                               ggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGAc                                                              >  1:27491/1‑139 (MQ=255)                                                                    tCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTg                                                         <  2:27491/139‑1 (MQ=255)                                                                       aTTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCg                                                      <  1:1371964/139‑1 (MQ=255)                                                                        ttCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGa                                                     <  2:800724/139‑1 (MQ=255)                                                                         ttcTGGCAGCCGGTATTTTATTGCTGGCATTTGTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAAGGCCGCGCTGATTGGACTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCaaa                                                    <  1:288996/137‑1 (MQ=255)                                                                             ggCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCg                                                                                                                         >  2:197680/1‑66 (MQ=255)                                                                             ggCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCg                                                                                                                         <  1:197680/66‑1 (MQ=255)                                                                                aGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCaaa                                             <  1:742452/139‑1 (MQ=255)                                                                                   cGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATg                                          <  2:487229/139‑1 (MQ=255)                                                                                              tGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTgg                                                                                                  <  1:216759/72‑1 (MQ=255)                                                                                              tGCTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTgg                                                                                                  >  2:216759/1‑72 (MQ=255)                                                                                                cTGGCATTTTTGCTACTGCGCGGACACAACCT‑CCCCCGCTACGTCCGCTGCTCAATGCCgcgc                                                                                                         >  2:309355/1‑63 (MQ=255)                                                                                                cTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCgcgc                                                                                                         <  1:309355/63‑1 (MQ=255)                                                                                                cTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGa                                                                                                      >  1:806271/1‑66 (MQ=255)                                                                                                cTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGa                                                                                                      <  2:806271/66‑1 (MQ=255)                                                                                                cTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGt                                                                                <  2:503873/88‑1 (MQ=255)                                                                                                cTGGCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGt                                                                                >  1:503873/1‑88 (MQ=255)                                                                                                  ggCATTTTTGCTACTGCGCGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATcg                           >  1:1370470/1‑139 (MQ=255)                                                                                                                 gcgcGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATgg                                                                       >  2:338853/1‑80 (MQ=255)                                                                                                                 gcgcGGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATgg                                                                       <  1:338853/80‑1 (MQ=255)                                                                                                                  cgcgGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGc           >  2:57662/1‑139 (MQ=255)                                                                                                                    cgGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTgg                                                                                                  >  2:495832/1‑50 (MQ=255)                                                                                                                    cgGACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTgg                                                                                                  <  1:495832/50‑1 (MQ=255)                                                                                                                      gACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACa                                                  <  1:1310660/96‑1 (MQ=255)                                                                                                                      gACACAAACT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACa                                                  >  2:1310660/1‑96 (MQ=255)                                                                                                                           aaaCT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  2:917318/139‑1 (MQ=255)                                                                                                                           aaaCT‑CCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  2:1370470/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                       GGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGCC  >  NC_000913/2029597‑2029858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑