| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,484,897 | (C)6→7 | coding (524/3594 nt) | evgS → | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,484,891 | 1 | . | C | 95.2% | 135.6 / ‑0.2 | 42 | coding (518/3594 nt) | evgS | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (21/19); total (22/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
CTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAG > NC_000913/2484753‑2485023 | cTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa < 2:130073/139‑1 (MQ=255) gccgccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑c‑ccc < 1:1127076/139‑1 (MQ=255) ccgccTCTTAATAATGAGATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑c‑cccc > 1:1050718/1‑139 (MQ=255) ccgccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑c‑cccc > 2:999859/1‑139 (MQ=255) ccgccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑c‑cccc < 2:1318965/139‑1 (MQ=255) cgccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑c‑ccccc > 1:260554/1‑139 (MQ=255) tctTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑c‑ccccccaga < 1:380787/139‑5 (MQ=255) ctTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑c‑ccccccagac < 2:1326444/139‑6 (MQ=255) tataagagacagttGCTGCAACCAAACCATTGATAATTACGTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCATAACCACTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACg < 2:914722/127‑1 (MQ=255) cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑c‑ccccccagac > 1:1226200/1‑123 (MQ=255) cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑c‑ccccccagac < 2:1226200/128‑6 (MQ=255) ttGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATtcatc > 2:960346/1‑139 (MQ=255) ctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCCAGACGAGGTAATTCatcaa > 1:1175219/1‑139 (MQ=255) aaaccaTTGATAATTGCCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTACTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATtcatctgtctcttata > 1:914722/1‑128 (MQ=255) aaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCa < 2:1050718/139‑1 (MQ=255) aTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAg < 2:1175219/139‑1 (MQ=255) ttACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTa > 1:348931/1‑139 (MQ=255) tACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt > 1:1284699/1‑139 (MQ=255) aCCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtc < 2:348931/139‑1 (MQ=255) tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAg > 2:690033/1‑123 (MQ=255) tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAg < 1:690033/123‑1 (MQ=255) aaCCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTtata < 2:260554/139‑1 (MQ=255) ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGc > 1:24671/1‑139 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt > 2:617616/1‑139 (MQ=255) gACTGAATGCAACCGCTTACCTCACCAAAGCCAGTAAGTAATGCTCAGGTACCAAATTAC‑C‑CCCCCAGTCGAGGTAATTCATCAATCATTTCCAAAAGTATCAAGTATCACTTTTACAAATTTATATCAGGCATTAGCa < 1:362996/139‑1 (MQ=255) gACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa > 1:268428/1‑139 (MQ=255) tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATcc > 1:1368582/1‑139 (MQ=255) tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑c‑ccccccaga > 2:685149/1‑58 (MQ=255) tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑c‑ccccccaga < 1:685149/62‑5 (MQ=255) gACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAACAATTTCCAAAGGCAACAATTACCTCTTTTACAATTTTATATAAGCCATTAGCATCCTTCTCAg > 1:818254/1‑139 (MQ=255) cGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt < 2:10658/95‑1 (MQ=255) cGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt > 1:10658/1‑95 (MQ=255) cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTggg > 2:381758/1‑139 (MQ=255) ttACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAAGTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc < 1:864438/139‑1 (MQ=255) cAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGc < 1:475594/74‑1 (MQ=255) cAAAACCAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGc > 2:475594/1‑74 (MQ=255) cAGTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTa < 1:960346/139‑1 (MQ=255) cAGTAAATATTGCTCGGGTAGCAAATTA‑‑CACCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACTAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTa < 2:923899/139‑1 (MQ=255) gTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAtt > 2:1052664/1‑139 (MQ=255) gTAAATATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAtt > 2:282652/1‑139 (MQ=255) aaaTATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAtt > 1:703169/1‑129 (MQ=255) aaaTATTGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAtt < 2:703169/129‑1 (MQ=255) ttGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagta < 2:1368582/139‑1 (MQ=255) ttGCTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagta < 2:1284699/139‑1 (MQ=255) ttGCTCGGGTAGCAAATTA‑‑‑‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAc > 2:1360735/1‑139 (MQ=255) cTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa < 1:871366/96‑1 (MQ=255) cTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa > 2:871366/1‑96 (MQ=255) cTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca < 2:24671/139‑1 (MQ=255) cTCGGGTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca > 1:67533/1‑139 (MQ=255) gTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTAc > 2:282246/1‑69 (MQ=255) gTAGCAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTAc < 1:282246/69‑1 (MQ=255) cAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaa > 2:1216579/1‑49 (MQ=255) cAAATTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaa < 1:1216579/49‑1 (MQ=255) aaaTTAC‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACcag < 1:1052664/139‑1 (MQ=255) | CTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑‑C‑CCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAG > NC_000913/2484753‑2485023 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |