| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,767,445 | (C)7→8 | intergenic (+90/‑265) | rnlB → / → yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,767,438 | 1 | . | C | 93.1% | 82.3 / ‑0.5 | 30 | intergenic (+83/‑272) | rnlB/yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (13/14); total (16/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑C‑CCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGT > NC_000913/2767303‑2767565 | ccTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑c‑cc > 2:687006/1‑139 (MQ=255) tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑c‑cccc < 1:687006/139‑1 (MQ=255) ccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CCCCCCTCACAACGTc < 1:166312/139‑1 (MQ=255) gatgatGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTAGAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CCCCCCTCACAACGTCCt < 1:1100717/139‑1 (MQ=255) cAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CCCCCCTCACAACGTCCTGCATAGGGAaca > 1:346678/1‑139 (MQ=255) aGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CCCCCCTcaca < 1:219971/118‑1 (MQ=255) aGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CCCCCCTcaca > 2:219971/1‑118 (MQ=255) tCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc < 2:346678/139‑1 (MQ=255) aCTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACt < 1:598308/139‑1 (MQ=255) aCGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAATC‑CCCCCCCTACAACGTCCTCCATAGAGAAACCGTTTTCCCCTTTGCACCCCCGACTCACTTTCCCCCCCt > 1:196113/1‑139 (MQ=255) ccAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCTCCCCCTTTaaa < 2:522744/139‑1 (MQ=255) aaTTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc < 2:149752/95‑1 (MQ=255) aaTTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc > 1:149752/1‑95 (MQ=255) tCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CCCCCCTcaca < 1:121025/64‑1 (MQ=255) tCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CCCCCCTcaca > 2:121025/1‑64 (MQ=255) cGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑CACCCCCCTCACAACGTCCAGCATACGTAACACATTTTCCCATGTGGATCCACGACTAAATTTCACCCCCTTTAAAATACTCAATTATg > 2:242181/1‑139 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CCCCCCTCACAACGTCCTGCATAGGGAac > 1:606902/1‑80 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CCCCCCTCACAACGTCCTGCATAGGGAac < 2:606902/80‑1 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGt < 2:40065/139‑1 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGt < 2:196113/139‑1 (MQ=255) tGATATTAACTTGTCCAATATGATCAAATAGCATTAACC‑CCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCTCTCTTTAAACTTCTCAATAATGTCACCAATTTTCt > 1:372932/1‑139 (MQ=255) gTCCAATATGATCAAATAGCATTAACC‑CCCCCCTcaca > 2:1115293/1‑38 (MQ=255) gTCCAATATGATCAAATAGCATTAACC‑CCCCCCTcaca < 1:1115293/38‑1 (MQ=255) gTCCAATATGATCAAATAGCATTAACC‑CCCCCCTCACAACGTCCTGCCTAGGGAACACGTTTTCCCCTGTCCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATTTCACCAATTTTCTGGAAATCCTatg > 1:801574/1‑139 (MQ=255) gTCCAATATGATCAAATAGCATTAACC‑CCCCCCTCACAACGTCCTGCATAAGGAACACGTCTTCCCCTGTGCACCCACGACTAAATTTACCCCCCTTTAAACTTCACAATAATGTCACCAATTTTCGGGCATTCCTatg > 1:1220994/1‑139 (MQ=255) tCCAATATGATCAAATAGCATTAACC‑CCCCCCTCACAACGTCCTGCATAGGGACCCCGCATTCCCATTTGCACCCCCGACTAAATTTACCACCCTTTAAACTTCTCAATAATGTCAACAATTTTCTGGAAATCTTatac > 2:488129/1‑137 (MQ=255) tCCAATATGATCAAATAGCATTAACC‑CCCCCCTCACAACGTCCTGCACAGGGAACACATTTTCCCCTGTGAACCCACGACTACACTTAACCCCCCTTAAAGTTTTCCATAATCTCTGCAATTTACTCGACATCCTatga > 1:286433/1‑139 (MQ=255) tCCAATATGATCAAATAGCATTAA‑C‑CCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTACACTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAGTGTCACCAATTTTCTGGAAATCCTatgat > 1:881901/1‑139 (MQ=255) tCAAATAGCATTAACC‑CCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTccc > 1:303233/1‑139 (MQ=255) tCAAATAGCATTAACC‑CCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAAATCCCCCCCTTTAAACTTATCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTccc > 1:1266513/1‑139 (MQ=255) cAAATAGCATTAACC‑CCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCg < 2:811540/139‑1 (MQ=255) aaTAGCATTAACC‑CCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGt < 2:286433/139‑1 (MQ=255) | CCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑C‑CCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGT > NC_000913/2767303‑2767565 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |