| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,101,039 | (C)7→8 | coding (585/720 nt) | yggN ← | DUF2884 family putative periplasmic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,101,032 | 1 | . | C | 97.1% | 110.9 / ‑3.2 | 34 | coding (592/720 nt) | yggN | DUF2884 family putative periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (13/20); total (13/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01 | |||||||||||
AATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCC > NC_000913/3100895‑3101138 | aaTAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑c < 2:185235/139‑1 (MQ=255) gagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccccc > 2:1340394/1‑139 (MQ=255) aaaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTccc < 2:1281307/139‑1 (MQ=255) aaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCa < 2:664079/139‑1 (MQ=255) tCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGca < 2:1321888/139‑1 (MQ=255) ccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGcaca > 1:265852/1‑139 (MQ=255) ggCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTaa > 1:216976/1‑139 (MQ=255) ggCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTaa > 2:1013508/1‑139 (MQ=255) tGCGGCTATCTTCCATAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccccccccagg < 1:861945/114‑5 (MQ=255) tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑ccccccccagg > 2:861945/1‑110 (MQ=255) tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cccccccca < 1:474143/112‑3 (MQ=255) tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cccccccca > 2:474143/1‑110 (MQ=255) tCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg < 1:640546/139‑1 (MQ=255) tCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTGTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg < 1:1340394/139‑1 (MQ=255) tCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCtt < 2:265852/139‑1 (MQ=255) tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTTCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCCCCCCAGGCTTCCCAGCACAtt < 2:1209740/111‑1 (MQ=255) tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GCCCCCCCCCAGGCTTCCCAGCACAtt > 1:1209740/1‑111 (MQ=255) tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACAt < 1:638604/109‑1 (MQ=255) tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACAt > 2:638604/1‑109 (MQ=255) tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCa > 2:436060/1‑139 (MQ=255) tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCa > 1:1297173/1‑139 (MQ=255) acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAcc > 2:568937/1‑139 (MQ=255) gCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgc > 1:7170/1‑139 (MQ=255) cTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg < 1:1013508/139‑1 (MQ=255) cTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg < 2:216976/139‑1 (MQ=255) aaCATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt < 2:7170/139‑1 (MQ=255) cTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAt < 1:523318/139‑1 (MQ=255) tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg > 1:597575/1‑105 (MQ=255) tGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg < 2:597575/105‑1 (MQ=255) ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTg > 2:504916/1‑139 (MQ=255) ggAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTa < 2:1033928/139‑1 (MQ=255) ggAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTa < 1:568937/139‑1 (MQ=255) tCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTccc > 2:1035225/1‑61 (MQ=255) tCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTccc < 1:1035225/61‑1 (MQ=255) cTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTc < 1:436060/139‑1 (MQ=255) ccTGCTTTTTCCCCTCGGTTTTGATTGAGGATTGCACC‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCa < 1:746410/139‑1 (MQ=255) cTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGc < 1:504916/139‑1 (MQ=255) tttttCCACTCGGTTTGGATTGAGGATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGcc < 2:1057772/139‑1 (MQ=255) tCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cccccccca > 2:287842/1‑35 (MQ=255) tCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑cccccccca < 1:287842/37‑3 (MQ=255) ggATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTa > 2:353652/1‑99 (MQ=255) ggATTGCA‑GC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTa < 1:353652/99‑1 (MQ=255) | AATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCA‑G‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCC > NC_000913/3100895‑3101138 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |