Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,154,109	G→A	*270* (TAG→TAA)	pabC →	4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,154,109	0	G	A	100.0%	182.9 / NA	57	*270* (TAG→TAA)	pabC	4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/28);  total (29/28)

TCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATCAAAGAAGAGACGATATTTACCCTG  >  NC_000913/1153975‑1154243                                                                                                                                        |                                                                                                                                       tCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCAt                                                                                                                                    >  2:809835/1‑139 (MQ=255)   gTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGa                                                                                                                                  >  1:412368/1‑139 (MQ=255)      gcaggcagATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGaaaa                                                                                                                               <  2:585451/139‑1 (MQ=255)        aggcagATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGaaaaaa                                                                                                                             <  2:1000270/139‑1 (MQ=255)           cagATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAgtg                                                                                                                          <  1:1303139/139‑1 (MQ=255)            agatgagatgGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAgtgt                                                                                                                         <  1:809835/139‑1 (MQ=255)            agatgagatgGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAgtgt                                                                                                                         <  2:751594/139‑1 (MQ=255)              atgagatgGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTa                                                                                                                       >  2:1166484/1‑139 (MQ=255)                    tgGTTATTTGTATTGCGTTAATGCCAGTGATACACGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATCTTAATATTTAGCCCCGCTTTGTGCGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATa                                                                                                                 >  1:1316965/1‑139 (MQ=255)                      gTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAAt                                                                                                               <  1:397328/139‑1 (MQ=255)                         aTTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGaa                                                                                                                                 >  2:524891/1‑118 (MQ=255)                         aTTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGaa                                                                                                                                 <  1:524891/118‑1 (MQ=255)                         aTTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCtt                                                                                                            >  1:393146/1‑139 (MQ=255)                                aTGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTg                                                                                                     >  2:484671/1‑139 (MQ=255)                                 tGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTg                                                                                                     >  1:714153/1‑139 (MQ=255)                                 tGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGc                                                                                                    <  2:412368/139‑1 (MQ=255)                                   cGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtg                                                                                                  >  1:152876/1‑139 (MQ=255)                                    gTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATCATCTTGTTATTGCtgg                                                                                                 <  2:1316965/139‑1 (MQ=255)                                      tAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtggtg                                                                                               <  1:484671/139‑1 (MQ=255)                                      tAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtggtg                                                                                               <  2:152876/139‑1 (MQ=255)                                        aTGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGa                                                                                                                   <  2:1001983/117‑1 (MQ=255)                                        aTGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGa                                                                                                                   >  1:1001983/1‑117 (MQ=255)                                            cAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTg                                                                                         <  1:1166484/139‑1 (MQ=255)                                                 aTGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTAt                                                                                    <  1:1185190/139‑1 (MQ=255)                                                  tGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTAt                                                                                    <  2:714153/139‑1 (MQ=255)                                                  tGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATc                                                                                   >  1:707015/1‑139 (MQ=255)                                                   gCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAAt                                                                                                               <  2:447240/110‑1 (MQ=255)                                                   gCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAAt                                                                                                               >  1:447240/1‑110 (MQ=255)                                                   gCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATcg                                                                                  >  2:334350/1‑139 (MQ=255)                                                      cGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATcgccg                                                                               <  1:602006/139‑1 (MQ=255)                                                         aTGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTg                                                                            >  2:792704/1‑139 (MQ=255)                                                             gCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGgtgt                                                                        >  2:279041/1‑139 (MQ=255)                                                               cTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTCTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTgggg                                                                      <  1:792704/139‑1 (MQ=255)                                                                 gtgGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGc                                                                    >  1:405153/1‑139 (MQ=255)                                                                                  t‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGtt                                                                                                                        >  2:1280879/1‑70 (MQ=255)                                                                                  t‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGtt                                                                                                                        <  1:1280879/70‑1 (MQ=255)                                                                                             cGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGAc                                         >  1:682599/1‑139 (MQ=255)                                                                                                  tatGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCaa                                    >  2:1334028/1‑139 (MQ=255)                                                                                                        tatTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCg                                                      >  1:606871/1‑115 (MQ=255)                                                                                                        tatTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCg                                                      <  2:606871/115‑1 (MQ=255)                                                                                                         atTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGa                                          <  2:1193176/126‑1 (MQ=255)                                                                                                         atTTAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGa                                          >  1:1193176/1‑126 (MQ=255)                                                                                                            tAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATc                          >  2:1226634/1‑139 (MQ=255)                                                                                                            tAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATc                          >  1:1242537/1‑139 (MQ=255)                                                                                                            tAGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATc                          <  2:393146/139‑1 (MQ=255)                                                                                                             aGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCt                              <  1:892134/134‑1 (MQ=255)                                                                                                             aGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATCa                         >  2:904572/1‑139 (MQ=255)                                                                                                             aGCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGATATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCt                              >  2:892134/1‑134 (MQ=255)                                                                                                              gCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtg                                                                                                  <  2:360903/65‑1 (MQ=255)                                                                                                              gCCCCACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtg                                                                                                  >  1:360903/1‑65 (MQ=255)                                                                                                                  cACTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATCAaagaa                    <  2:682599/139‑1 (MQ=255)                                                                                                                   aCTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATCAaagaag                   >  1:552367/1‑139 (MQ=255)                                                                                                                    cTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGc                                                                                                    >  2:645866/1‑57 (MQ=255)                                                                                                                    cTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGc                                                                                                    <  1:645866/57‑1 (MQ=255)                                                                                                                    cTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtg                                                                                                  >  1:735812/1‑59 (MQ=255)                                                                                                                    cTTTGTGAGCGCCCGAATTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtg                                                                                                  <  2:735812/59‑1 (MQ=255)                                                                                                                                    aTTAATCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATCAAAGAAGAGACGATATTTACCCTg  <  1:1226634/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTT‑CGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCGTCTGGAAGGTTCGCCATCTTGCCGACAGCAAATTGCTTATCAAAGAAGAGACGATATTTACCCTG  >  NC_000913/1153975‑1154243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑