Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,682,095	A→G	intergenic (‑65/‑64)	ydgC ← / → rstA	GlpM family inner membrane protein/response regulator of RstAB two‑component system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,682,095	0	A	G	100.0%	175.1 / NA	50	intergenic (‑65/‑64)	ydgC/rstA	GlpM family inner membrane protein/response regulator of RstAB two‑component system
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/23);  total (27/23)

TTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGATTACCAGCCCCATCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAACGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTATGAACACTATCGTATTTGTGGAAGATGATGCGGAAGTCGGTTCAC  >  NC_000913/1681967‑1682204                                                                                                                                 |                                                                                                               ttGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGATTACCAGCCCCATCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATag                                                                                                      <  1:696931/139‑1 (MQ=255)              aaTCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGATTACCAGCCCCATCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAg                                                                                         >  1:367217/1‑139 (MQ=255)                   aCAGCACTACCAGCGCACCGAGCGCGGCTTTGATTACCAGCCCCATCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAgc                                                                                        <  2:149662/135‑1 (MQ=255)                   aCAGCACTACCAGCGCACCGAGCGCGGCTTTGATTACCAGCCCCATCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAgc                                                                                        >  1:149662/1‑135 (MQ=255)                       cACTACCAGCGCACCGAGCGCGGCTTTGATTACCAGCCCCATCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGcgcg                                                                                     <  1:1149622/134‑1 (MQ=255)                       cACTACCAGCGCACCGAGCGCGGCTTTGATTACCAGCCCCATCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGcgcg                                                                                     >  2:1149622/1‑134 (MQ=255)                            ccAGCGCACCGAGCGCGGCTTTGATTACCAGCCCCATCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATtgtg                                                                           >  2:992274/1‑139 (MQ=255)                                      gAGCGCGGCTTTGATTACCAGCCCCATCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTtat                                                                 >  1:673018/1‑139 (MQ=255)                                       aGCGCGGCTTTGATTACCAGCCCCATCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTtata                                                                >  2:583175/1‑139 (MQ=255)                                            ggCTTTGATTACCAGCCCCATCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTAc                                                           <  2:673018/139‑1 (MQ=255)                                                 tGATTACCAGCCCCATCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑a                                                     <  1:992274/139‑1 (MQ=255)                                                   aTTACCAGCCCCATCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AAt                                                   <  1:241186/139‑1 (MQ=255)                                                        cAGCCCCATCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTCTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGAG‑ATTGTTAt                                              >  1:934288/1‑139 (MQ=255)                                                           ccccATCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTATGaa                                           >  1:500421/1‑139 (MQ=255)                                                             ccATCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTATGAaca                                         >  2:1275697/1‑139 (MQ=255)                                                              cATCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATtgtg                                                                           <  2:1169777/105‑1 (MQ=255)                                                              cATCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATtgtg                                                                           >  1:1169777/1‑105 (MQ=255)                                                               aTCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTATGAACACt                                       >  2:300518/1‑139 (MQ=255)                                                                tCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTATGAACACTa                                      >  2:1029898/1‑139 (MQ=255)                                                                 cTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTATGAACACTAt                                     <  2:367217/139‑1 (MQ=255)                                                                      tACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTATGAACACTATCGTAt                                <  1:300518/139‑1 (MQ=255)                                                                           tAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGcgcg                                                                                     >  2:438335/1‑82 (MQ=255)                                                                           tAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGcgcg                                                                                     <  1:438335/82‑1 (MQ=255)                                                                            aaCACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATc                                                              >  1:845925/1‑104 (MQ=255)                                                                            aaCACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATc                                                              <  2:845925/104‑1 (MQ=255)                                                                            aaCACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTAt                                              >  1:1293785/1‑119 (MQ=255)                                                                            aaCACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTAt                                              <  2:1293785/119‑1 (MQ=255)                                                                                   ccATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTTTA‑CTTCCGTGAAAGGTAATAAAACCAATGGTATTTGTGGAGgttga                   >  2:173246/1‑139 (MQ=255)                                                                                    cATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTATGAACACTATCGTATTTGTGGAAgatgat                  >  1:263665/1‑139 (MQ=255)                                                                                       aaCAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTATGAACACTATCGTATTTGTGGAAGATGATGCg               <  2:500421/139‑1 (MQ=255)                                                                                           aGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑a                                                     <  1:197486/97‑1 (MQ=255)                                                                                           aGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑a                                                     >  2:197486/1‑97 (MQ=255)                                                                                           aGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTATGAACACTATCGTATTTGTGGAAGATGATGCGGAAg           >  2:129917/1‑139 (MQ=255)                                                                                            gTCATCAGTAGAATACCTGATGAAAACTTGTATAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTATGAACACTATCGTATTTGTGGAAGATGATGCGGAAGt          >  1:928027/1‑139 (MQ=255)                                                                                              catcaGTAGAATACCTCATGAAAACTTGTTTAGAAGCGATTGATCGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACTGTG‑AATGTTATGAACACTATCGTATTTGTGGAAGATGATGCGGAAGTCg        >  1:340169/1‑139 (MQ=255)                                                                                                tcaGTAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTATGAACACTATCGTATTTGTGGAAGATGATGCGGAAGTCGGt      <  1:129917/139‑1 (MQ=255)                                                                                                tcaGTAGAATACCTGATGAAAACTTGTATAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTATGAACACTATCGTATTTGTGGAAGATGATGCGGAAGTCGGt      <  2:928027/139‑1 (MQ=255)                                                                                                    tAGAATACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTATGAACACTATCGTATTTGTGGAAGATGATGCGGAAGTCGGTTCAc  <  2:550700/139‑1 (MQ=255)                                                                                                       aaTACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATg                                                  <  2:592324/88‑1 (MQ=255)                                                                                                       aaTACCTGATGAAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATg                                                  >  1:592324/1‑88 (MQ=255)                                                                                                              gatgaAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATtgtg                                                                           >  1:1120992/1‑57 (MQ=255)                                                                                                              gatgaAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATtgtg                                                                           <  2:1120992/57‑1 (MQ=255)                                                                                                              gatgaAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGa                                                                          >  2:119019/1‑58 (MQ=255)                                                                                                              gatgaAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGa                                                                          <  1:119019/58‑1 (MQ=255)                                                                                                              gatgaAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGAc                                                                         >  2:808309/1‑59 (MQ=255)                                                                                                              gatgaAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGAc                                                                         <  1:808309/59‑1 (MQ=255)                                                                                                              gatgaAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTATGAACACTATCGTATTTGTGGAAGATGATGCGGAAGTCGGt      <  2:1057835/125‑1 (MQ=255)                                                                                                              gatgaAAACTTGTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTATGAACACTATCGTATTTGTGGAAGATGATGCGGAAGTCGGt      >  1:1057835/1‑125 (MQ=255)                                                                                                                         gTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTATGAACACTATc                                    <  2:229411/84‑1 (MQ=255)                                                                                                                         gTTTAGAAGCGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTATGAACACTATc                                    >  1:229411/1‑84 (MQ=255)                                                                                                                                 |                                                                                                               TTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGATTACCAGCCCCATCTTTTTACCTTAACACTTCCATAACAAGTCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAACGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTTTTATATCTACCGTG‑AATGTTATGAACACTATCGTATTTGTGGAAGATGATGCGGAAGTCGGTTCAC  >  NC_000913/1681967‑1682204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑