Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	183,620	G→A	*386* (TAG→TAA)	cdaR →	carbohydrate diacid regulon transcriptional regulator, autoregulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	183,620	0	G	A	100.0%	111.5 / NA	35	*386* (TAG→TAA)	cdaR	carbohydrate diacid regulon transcriptional regulator, autoregulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/12);  total (23/12)

TTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGAT  >  NC_000913/183492‑183753                                                                                                                                 |                                                                                                                                      ttgttTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTa                                                                                                                            >  2:636590/1‑139 (MQ=255)            tCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTa                                                                                                                                   <  2:177943/121‑1 (MQ=255)            tCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTa                                                                                                                                   >  1:177943/1‑121 (MQ=255)            tCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCATGATTTGGGCAATTTTGATGACAGGTTGCGGCTGTATGTGGCGTTACAAC‑GGGTGAAGAGCGGTAAGTTATGCGTTAAGGATGAggcg                                                                                                                 >  1:662557/1‑139 (MQ=255)                 aTACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCt                                                                                                            <  1:636590/139‑1 (MQ=255)                       tGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCa                                                                                                      >  2:629416/1‑139 (MQ=255)                            tATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTccc                                                                                                 >  2:116250/1‑139 (MQ=255)                              tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                               >  2:132294/1‑139 (MQ=255)                              tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                               >  2:256911/1‑139 (MQ=255)                              tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                               >  2:782269/1‑139 (MQ=255)                               cGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                              >  1:72100/1‑123 (MQ=255)                               cGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                              <  2:72100/123‑1 (MQ=255)                                   ttAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGcc                                                                                          >  1:169388/1‑139 (MQ=255)                                     aaTCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                                        <  1:1230435/139‑1 (MQ=255)                                            tatCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTa                                                                                 >  2:673256/1‑139 (MQ=255)                                                 gAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAggg                                                                            >  2:757074/1‑139 (MQ=255)                                                    cTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGaa                                                                         >  1:395940/1‑139 (MQ=255)                                                       aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                      >  2:636342/1‑139 (MQ=255)                                                                  tttGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAAcc                                                           <  1:629416/139‑1 (MQ=255)                                                                      ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCa                                                       >  2:1257806/1‑139 (MQ=255)                                                                      ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCa                                                       >  2:1187827/1‑139 (MQ=255)                                                                                atgaCAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGatt                                             >  2:1369259/1‑139 (MQ=255)                                                                                     aGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTa                                        >  2:1350997/1‑139 (MQ=255)                                                                                       gTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                      >  2:17318/1‑107 (MQ=255)                                                                                       gTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                      <  1:17318/107‑1 (MQ=255)                                                                                       gTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTAcg                                      >  2:744690/1‑139 (MQ=255)                                                                                              ctgTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGt                               <  1:132294/139‑1 (MQ=255)                                                                                                       gCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGa                      <  1:1257806/139‑1 (MQ=255)                                                                                                              aaCTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCtt               <  2:1214528/139‑1 (MQ=255)                                                                                                                   ggtGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAAt           <  2:169388/137‑1 (MQ=255)                                                                                                                   ggtGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAAt           >  2:1249124/3‑139 (MQ=255)                                                                                                                            cGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGtt                                                    <  1:950602/89‑1 (MQ=255)                                                                                                                            cGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGtt                                                    >  2:950602/1‑89 (MQ=255)                                                                                                                            cGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGAt  >  2:38652/1‑139 (MQ=255)                                                                                                                            cGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGAt  <  1:1369259/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                      TTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGAT  >  NC_000913/183492‑183753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑