Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,664,986	C→T	*275* (TAG→TAA)	gadX ←	acid resistance regulon transcriptional activator, autoactivator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,664,986	0	C	T	100.0%	161.3 / NA	50	*275* (TAG→TAA)	gadX	acid resistance regulon transcriptional activator, autoactivator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/26);  total (24/26)

AACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTCTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTCCTGATACTCTGTGGGCGTCATCCCA  >  NC_000913/3664859‑3665115                                                                                                                                 |                                                                                                                                  aaCTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTAt                                                                                                                        >  2:494800/1‑139 (MQ=255)    ttACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTAttcc                                                                                                                     <  2:553428/139‑1 (MQ=255)      aCTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTtata                                                                                                                               <  1:1161636/127‑1 (MQ=255)      aCTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTtata                                                                                                                               >  2:1161636/1‑127 (MQ=255)      aCTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTAt                                                                                                                        <  1:1157608/134‑1 (MQ=255)      aCTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTAt                                                                                                                        >  2:1157608/1‑134 (MQ=255)        tGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTAttcctt                                                                                                                   >  1:1040936/1‑137 (MQ=255)        tGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTAttcctt                                                                                                                   <  2:1040936/137‑1 (MQ=255)            agCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAg                                                                                                             >  1:1127462/1‑139 (MQ=255)              cacaAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGGTTTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGa                                                                                                            <  2:1291091/139‑1 (MQ=255)                  aaGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTTTTCCTTCCGCAGAACGGt                                                                                                       <  1:323499/139‑1 (MQ=255)                      ttCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTtat                                                                                                                                <  1:108242/110‑1 (MQ=255)                      ttCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTtat                                                                                                                                >  2:108242/1‑110 (MQ=255)                                      gAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCt                                                                                   <  1:295024/139‑1 (MQ=255)                                       aGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCtt                                                                                  >  1:347386/1‑139 (MQ=255)                                          gtTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTgcg                                                                               <  2:982579/139‑1 (MQ=255)                                          gtTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTgcg                                                                               <  1:494800/139‑1 (MQ=255)                                           ttATAACGGTTTATTGGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTgcgc                                                                              >  1:913369/1‑139 (MQ=255)                                                   gTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATAc                                                                      >  2:75226/1‑139 (MQ=255)                                                      tattaGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTg                                                                   >  2:286882/1‑139 (MQ=255)                                                             cTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTta                                                                                                                                 <  1:422424/70‑1 (MQ=255)                                                             cTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTta                                                                                                                                 >  2:422424/1‑70 (MQ=255)                                                              tGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTg                                                                                 >  1:1327830/1‑117 (MQ=255)                                                              tGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTg                                                                                 <  2:1327830/117‑1 (MQ=255)                                                                gagaCGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTc                                                         >  2:907243/1‑139 (MQ=255)                                                                    cGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACgg                                                     <  1:984800/139‑1 (MQ=255)                                                                              tCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAAtc                                           >  1:637005/1‑139 (MQ=255)                                                                                     cccGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTgcgc                                    >  1:597392/1‑139 (MQ=255)                                                                                     cccGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGAC‑ATCTCTGCGcc                                   >  1:248209/1‑139 (MQ=255)                                                                                      ccGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGcc                                   >  1:764624/1‑139 (MQ=255)                                                                                        ggTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAAt                                                                        >  1:542979/1‑100 (MQ=255)                                                                                        ggTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAAt                                                                        <  2:542979/100‑1 (MQ=255)                                                                                        ggTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGa                                 >  1:525922/1‑139 (MQ=255)                                                                                         gTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTta                                                                                                                                 >  1:293252/1‑42 (MQ=255)                                                                                         gTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGAt                                <  2:637005/139‑1 (MQ=255)                                                                                          ttcccTATGTCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATATTCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATc                               <  1:333590/137‑1 (MQ=255)                                                                                            cccTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGc                             <  2:913369/139‑1 (MQ=255)                                                                                            cccTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGc                             <  2:525922/139‑1 (MQ=255)                                                                                            cccTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGc                             <  2:1252494/139‑1 (MQ=255)                                                                                              cTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTc                           <  2:347386/139‑1 (MQ=255)                                                                                              cTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTc                           <  2:597392/139‑1 (MQ=255)                                                                                                      gg‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCTCAGAACGGTCAGTGCCGt                                                                                              <  1:759067/64‑1 (MQ=255)                                                                                                      gg‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGt                                                                                              >  2:759067/1‑64 (MQ=255)                                                                                                                tATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTCCTGATACTCTGTGGGCg          <  1:907243/139‑1 (MQ=255)                                                                                                                   gTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTCCTGATACTCTGTGGGCGTCa       <  1:40569/139‑1 (MQ=255)                                                                                                                        aGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTc                           >  2:1093246/1‑114 (MQ=255)                                                                                                                        aGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTc                           <  1:1093246/114‑1 (MQ=255)                                                                                                                        aGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTCCTGATACTCTGTGGGCGTCATCCCa  >  2:170685/1‑139 (MQ=255)                                                                                                                          tAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTgcg                                                                               <  1:1342525/60‑1 (MQ=255)                                                                                                                          tAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTgcg                                                                               >  2:1342525/1‑60 (MQ=255)                                                                                                                                 |                                                                                                                                  AACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTCTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTCCTGATACTCTGTGGGCGTCATCCCA  >  NC_000913/3664859‑3665115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑