Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	468,093	Δ1 bp	coding (40/339 nt)	glnK →	nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	468,092	0	G	.	100.0%	279.0 / NA	60	coding (39/339 nt)	glnK	nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (36/24);  total (36/24)

TCTTAACTTCCTGCTCTCTTTCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAA  >  CP009273/467950‑468233                                                                                                                                               |                                                                                                                                              tcttAACTTCCTGCTCTCTTTCTCGTTTTTCATTTCTGGCACACCGCGTGCAATACCTTCTTCGTTTGGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATAAAGCTGGGGACCGTGATATTAAAGCCATTCAAGCT‑GAAGACg                                                                                                                                        >  2:143594/1‑149 (MQ=255)      aCTTCCTGCTCTCTTTCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAGCCATTCAAGCT‑GAAGACGTTCGt                                                                                                                                   <  2:471285/149‑1 (MQ=255)       cTTCCTGCTCTCTTTCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTg                                                                                                                                  <  2:330205/149‑1 (MQ=255)          ccTGCTCTCTTTCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAg                                                                                                                               <  2:533123/149‑1 (MQ=255)           cggCTCTCTTTCTTGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGc                                                                                                                              <  1:383419/147‑1 (MQ=255)                ctctTTCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTAt                                                                                                                         <  2:790452/149‑1 (MQ=255)                 tctTTCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATc                                                                                                                        <  1:798598/149‑1 (MQ=255)                   tttCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCtt                                                                                                                      <  2:706577/149‑1 (MQ=255)                          tttttCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTGTCTTcctgtgg                                                                                                               >  1:183416/1‑144 (MQ=255)                              tCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTAtt                                                                                                           <  2:716999/149‑1 (MQ=255)                               cATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCAGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTc                                                                                                          <  1:647449/149‑1 (MQ=255)                                  ttCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCCgg                                                                                                       >  1:498861/1‑149 (MQ=255)                                    cTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGc                                                                                                     <  1:541841/149‑1 (MQ=255)                                       gCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTg                                                                                                  >  2:134303/1‑149 (MQ=255)                                       gCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGAGACAAGCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTg                                                                                                  >  2:874542/1‑149 (MQ=255)                                              gCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGTTAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGGTATCTTCCATTGGTATTCAGGGCCTGACCGTcg                                                                                           >  1:226679/1‑148 (MQ=255)                                                  gCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGa                                                                                       >  1:166284/1‑149 (MQ=255)                                                    aaTACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGGCGGAACGTCACCGAAg                                                                                     >  2:423265/1‑149 (MQ=255)                                                          ttcttcGTGTAGCAGAACCATTCCCAAATTCTGCGCGGAGGGGATCTATGTAGCTGGTGACCGTGATAATCAAACCATTCGAGCT‑GAAGACGTGCATGAAGCGTTATCTTCCGTTGGTCTTCGGGGCCTGCCCGTCACCGAAGTAAAAg                                                                               >  1:170348/1‑149 (MQ=255)                                                           tcttcGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATATTCCATTGGTATTCAGGGCCTGACCGTAACCGAACTGAAAgg                                                                              >  1:188699/1‑149 (MQ=255)                                                             ttcGTGGAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGtt                                                                            <  2:137579/149‑1 (MQ=255)                                                                gtgtAGCAGAACCATCACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCg                                                                         <  2:264480/149‑1 (MQ=255)                                                                 tgtAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATTTTCCATTGGTATTCAGGGGCTGACCGTCACCGAAGTGAAAGGTTTCgg                                                                        >  2:191619/1‑149 (MQ=255)                                                                   tAGCAGAACCATTACCGAATTCTGACCCAGGCATAAGTTTGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGGGGAAGGTTTGGGGc                                                                      >  2:216114/1‑149 (MQ=255)                                                                     gCAGAACCATGACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGt                                                                    <  1:143594/149‑1 (MQ=255)                                                                      cAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATGCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTc                                                                   <  1:775080/149‑1 (MQ=255)                                                                         aaCCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGa                                                                <  2:387018/149‑1 (MQ=255)                                                                         aaCCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCAATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGTTTTCGGGCGTCAGa                                                                >  2:306064/1‑149 (MQ=255)                                                                            cATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGACGTGAAAGGTTTCGGGCGTCAGAAAg                                                             >  2:795208/1‑149 (MQ=255)                                                                               tACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGc                                                          >  1:613123/1‑149 (MQ=255)                                                                                  cGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATg                                                       >  1:883394/1‑149 (MQ=255)                                                                                    aattgttagcGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGCCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGcc                                                     <  1:847044/140‑1 (MQ=255)                                                                                        cTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGc                                                 <  1:874542/149‑1 (MQ=255)                                                                                        cTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGc                                                 <  1:134303/149‑1 (MQ=255)                                                                                         tGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTTACCGTCACCGGAGTGAAAGGGTTCGGGCGTCAGAAAGGGCATGCCGAGCt                                                >  2:69714/1‑149 (MQ=255)                                                                                           aCCGGAGGGGATCTATGAAGCTGGTGACCGTGCTAATCAAACCATTCAAGCT‑GACGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGCCCGTCACCGAAGTGAAAGGTGTCGGGCGGCAGGAGGGGCATGCCGAGCTGt                                              >  1:691905/1‑149 (MQ=255)                                                                                              ggAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGCAAGGGCATGCCGAGCTGTAcc                                           >  1:440094/1‑149 (MQ=255)                                                                                                 ggggATCGATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCggg                                        <  1:557334/149‑1 (MQ=255)                                                                                                      tCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCg                                   >  2:363718/1‑149 (MQ=255)                                                                                                         aTGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTGCCGGGGGGCggga                                >  2:80069/1‑147 (MQ=255)                                                                                                          tGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAAt                               >  2:202164/1‑149 (MQ=255)                                                                                                              gCTGGTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAg                           <  2:613123/149‑1 (MQ=255)                                                                                                               gcgttctccGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGc                          <  1:443330/142‑1 (MQ=255)                                                                                                                  gTGACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTc                       >  2:885739/1‑149 (MQ=255)                                                                                                                    gACCGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGGCaa                     >  2:416008/1‑149 (MQ=255)                                                                                                                       cGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAAttt                  >  1:549190/1‑149 (MQ=255)                                                                                                                       cGTGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACAGGGGGGCGGAATACAGCGTCAAttt                  >  1:789878/1‑149 (MQ=255)                                                                                                                         tGATAATCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTcc                >  1:210672/1‑149 (MQ=255)                                                                                                                            tcaTCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGc             <  1:748457/147‑1 (MQ=255)                                                                                                                              aTCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCa           <  2:728544/149‑1 (MQ=255)                                                                                                                              aTCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGCGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCa           >  2:854782/1‑149 (MQ=255)                                                                                                                               tCAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGACGGAATACAGCGTCAATTTCCTGCCaa          >  2:274316/1‑149 (MQ=255)                                                                                                                                cAAACCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTACCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGCGAAAGGTGGCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGGCAATTTCCTGCCaaa         >  1:618650/1‑149 (MQ=255)                                                                                                                                 aaaCCATTCAAGCT‑GAAGACGTTCGTGAAGTGATGGCATCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCaaaa        >  2:657913/1‑149 (MQ=255)                                                                                                                                 aaaCCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCaaaa        >  1:407004/1‑149 (MQ=255)                                                                                                                                  aaCCATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAg       >  1:313357/1‑149 (MQ=255)                                                                                                                                    ccATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTa     >  2:257771/1‑149 (MQ=255)                                                                                                                                     cATTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTaa    >  2:134257/1‑149 (MQ=255)                                                                                                                                      aTTCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTaaa   >  2:703100/1‑149 (MQ=255)                                                                                                                                       ttCAAGCT‑GAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATGCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTaaaa  <  1:382736/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                              TCTTAACTTCCTGCTCTCTTTCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAA  >  CP009273/467950‑468233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑