Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,811,405	A→G	8.0%	intergenic (‑186/+13)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,811,405	0	A	G	8.0%	177.2 / 6.6	73	intergenic (‑186/+13)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (40/27);  new base G (2/4);  total (42/31)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.78e-01

AGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGT  >  CP009273/2811264‑2811550                                                                                                                                               |                                                                                                                                                  aGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCACCTCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                                                                                            <  2:722780/149‑1 (MQ=255)        tCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCAGTGCTGAAGATTTCGGAGAATATg                                                                                                                                     >  1:656587/1‑149 (MQ=255)        tCAATTAAGAGATGGTGCATCCGGGAGGATTACTACCATTACGGCACGGCGTTGGGGAGGTAAATAAAGCGACCTTATCATCCCTT‑GTGCGTGGTGTTAGTTCCGCTACAACTTCGATACCTTCCTTGCTGAAGATTTCGGGGaagaag                                                                                                                                     >  1:6069/1‑145 (MQ=12)          aaTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACTTTCATTGCTGAAGATTTGGGAGAATATGGt                                                                                                                                   >  1:601198/1‑149 (MQ=255)          aaTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGt                                                                                                                                   >  2:241282/1‑149 (MQ=255)          aaTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTAGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGt                                                                                                                                   >  2:579389/1‑149 (MQ=255)            ttAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTT‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGc                                                                                                                                 >  2:269648/1‑149 (MQ=255)            ttAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGc                                                                                                                                 >  2:184348/1‑149 (MQ=255)            ttAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGc                                                                                                                                 >  2:240402/1‑149 (MQ=255)            ttAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTAGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGc                                                                                                                                 >  2:598205/1‑149 (MQ=255)              aaGAGATGGTGCGTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCAGCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGgagatggttttgcat                                                                                                                               <  1:149305/149‑12 (MQ=17)              aaGAGACGTGGCATCCGGGAGTCTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATTCTACAAATGTG‑CTTCTTGTAATTCCGCTACCTTTTCGATGCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCAt                                                                                                                               <  2:25527/149‑1 (MQ=12)                     ggTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTTCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGGGAAGATGGTGCATCCGggag                                                                                                                        >  2:29674/1‑149 (MQ=255)                          aTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTc                                                                                                                   <  2:572652/149‑1 (MQ=255)                           tCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTA‑CTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAGGATTTCGGAGAATATGGTGCATCCGggagggctcg                                                                                                                  >  1:724387/1‑144 (MQ=255)                            ccGGGAGGATTCCTCGCCTCGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGa                                                                                                                 <  2:827792/149‑1 (MQ=255)                               ggaggaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAAcc                                                                                                              <  2:526536/149‑1 (MQ=255)                                 acgaTTTCACGCCCTGCCGCTCGCCCTTCGGGCCGTTGCTAACGCAACGTTATCCTTCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTc                                                                                                            <  1:118270/147‑1 (MQ=14)                                     ttACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGAc                                                                                                        >  1:134848/1‑149 (MQ=255)                                           gCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCg                                                                                                  >  1:579827/1‑149 (MQ=255)                                            ccctGCCGCTCGCCCTTCGGGCCGTTGCTAACGCAACGTTATCCTCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCgg                                                                                                 <  1:281713/146‑1 (MQ=14)                                              ttGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGtt                                                                                               >  1:131636/1‑149 (MQ=255)                                              ctGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCTTTGCTGAAGATTTCGGAGAATAGGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGtt                                                                                               <  1:532772/148‑1 (MQ=255)                                               tGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCTTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                                                              <  2:70110/149‑1 (MQ=255)                                               tGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCCCGGTTc                                                                                              >  1:800722/1‑149 (MQ=255)                                               agCGACTCGCCCTTAAGAACGGTGCAAAAGCAACGTTATCATCCCTC‑GTGACTACGGTTAATTCCACTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGACCATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCGCGGTTc                                                                                              >  1:727167/2‑149 (MQ=17)                                                gCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCg                                                                                             <  2:697496/149‑1 (MQ=255)                                                gCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCg                                                                                             <  2:365224/149‑1 (MQ=255)                                                 cGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCGTCCCTA‑GTGCCTGCGGTTGATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGt                                                                                            >  1:187820/1‑149 (MQ=255)                                                  ggCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                                                                           >  1:93394/1‑149 (MQ=255)                                                            tCGGGCCGTTGCTAAAGCAACGGTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTGCCATCCCTTCATTGCCGAAGATTTCGGTGATGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAct                                                                                 <  2:248036/149‑1 (MQ=17)                                                                        tAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCGGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCGATCCAGCTGa                                                                     >  1:484333/1‑149 (MQ=255)                                                                        tAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa                                                                     <  1:579389/149‑1 (MQ=255)                                                                        tAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa                                                                     <  1:241282/149‑1 (MQ=255)                                                                         aaaGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                    >  2:431312/1‑149 (MQ=255)                                                                         aaaGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                    >  2:87413/1‑149 (MQ=255)                                                                              accggtATCAGCCCTA‑GGGGCTGCGGTTAATTCCGCCACCTCTTCGATCCCTTCATTGCTGAAGATTTCGGCGAATAGGGTGCATCCGGGAGGATTCGAACCTCCGCCCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTggatagag                                                               <  1:41981/144‑8 (MQ=14)                                                                                      catcCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATc                                                       >  2:247294/1‑149 (MQ=255)                                                                                       atcCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGCGTCCTCTATCCAGCTGAGCTACGGATGCATCg                                                      >  1:425239/1‑149 (MQ=255)                                                                                       atcCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGCGCTCCGGATGCATCg                                                      >  2:438306/1‑149 (MQ=255)                                                                                       atcCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                                                      >  2:617314/1‑149 (MQ=255)                                                                                        tcCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCgg                                                     >  2:244766/1‑149 (MQ=255)                                                                                         cccTC‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATATCTTCATTGATGAAGATTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCggg                                                    <  2:593808/149‑1 (MQ=255)                                                                                         cccTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCggg                                                    >  1:775829/1‑149 (MQ=255)                                                                                         cccTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGCTCGTAGCCGAGGACTCTATCCAGCTGAGCGACGGAGGCAGCggg                                                    >  1:157418/1‑149 (MQ=14)                                                                                            tA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGATCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaa                                                 <  2:579827/149‑1 (MQ=255)                                                                                                     gCGGTTAATTCCGCTACCTCTTCGATACATTCATGGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGGCCGTAGCCGAGGACTCGATCCAGCTGAGCTACGGATGCATCGGGAAATTTAtttt                                         >  1:298276/1‑149 (MQ=14)                                                                                                        gTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATttactttact                                      >  2:8157/1‑149 (MQ=255)                                                                                                         ttAATTCCGCTCCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActg                                     <  2:601198/149‑1 (MQ=255)                                                                                                           aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgct                                   >  1:81663/1‑149 (MQ=255)                                                                                                              tCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCCCTCGGTTCGTAGCCGAGAAAGATGACGTTCTGAGCTAAGGGTCCATCTGGAAATTGGTTTTACTGCTGat                                >  2:629091/1‑149 (MQ=14)                                                                                                                tggtcccttctCGATTCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCAGGAAATTTATTTTACTGCTGatat                              <  2:646052/139‑1 (MQ=14)                                                                                                                cGCTATCTCTTCGATACCTTCATTGCTGAAGGTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGatat                              >  1:164826/1‑149 (MQ=255)                                                                                                                cGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGatat                              <  1:126083/149‑1 (MQ=12)                                                                                                                 gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATAtt                             <  1:240402/149‑1 (MQ=255)                                                                                                                   tACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCCCCGACCCCCCGGTTCGTAGCCGAGGCCTCAACCCCGCTGACCTACGAACGCATCGGGAAACATATTTTACTGCAGAAAttta                           >  1:220473/1‑147 (MQ=14)                                                                                                                     ccTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATa                         <  2:278073/149‑1 (MQ=255)                                                                                                                       tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACgg                                                              <  2:720182/110‑1 (MQ=35)                                                                                                                       tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATGTTGATAct                       >  2:489171/1‑148 (MQ=255)                                                                                                                       tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATAcc                       >  2:188114/1‑149 (MQ=255)                                                                                                                          tggagaCCTTCATTGCGGAAGATTTCGGTGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGct                    <  2:405905/144‑1 (MQ=255)                                                                                                                          tcgagcgctccATGGCTGAAGATTTCGGAGAATATGGGGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTATTTTACTGCTGATATTGATACCGct                    <  2:59723/139‑1 (MQ=14)                                                                                                                                ccTTCATTGCTGAAGGTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTaaa              <  2:164826/149‑1 (MQ=255)                                                                                                                                ccTTCATGGCGTAAGGTTTCGGAGAATATGGTGCATCCGGGAGGATTCGACCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTaaa              <  2:504808/149‑1 (MQ=14)                                                                                                                                  ttCATTGCTGAAGGTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAGTTTATTTTACTGCTGATATTGATACCg                      <  2:26352/139‑1 (MQ=255)                                                                                                                                  ttCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAGTTTATTTTACTGCTGATATTGATACCg                      >  1:26352/1‑139 (MQ=255)                                                                                                                                  ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGTGCCATAGTTTACTGCTGATATTGATACCGCTACTAAAGc            <  2:425251/149‑1 (MQ=14)                                                                                                                                   tCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACg                                                               <  2:527854/97‑1 (MQ=38)                                                                                                                                     aTTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCat         >  2:747736/1‑149 (MQ=255)                                                                                                                                         cTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTCAAGCCCTATCa     >  2:134701/1‑149 (MQ=255)                                                                                                                                          tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATGTTGATACCGCGACTAAAGCCATATCaa    >  2:701915/1‑149 (MQ=255)                                                                                                                                          tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCaa    >  2:306332/1‑149 (MQ=255)                                                                                                                                            aaGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATGCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATGTTTATACCGCTACTAAAGCCCTCTCAAGt  >  2:270616/1‑149 (MQ=14)                                                                                                                                               |                                                                                                                                                  AGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTA‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGT  >  CP009273/2811264‑2811550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑