Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	CP009273	299,113	IS5 (–) +4 bp	coding (412‑415/615 nt)	yagU →	DUF1440 family inner membrane protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	269666 =	NA (NA)	49 (1.090)	30/296	0.6	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	CP009273	= 299116	0 (0.000)					coding (415/615 nt)	yagU	DUF1440 family inner membrane protein
*	?	CP009273	= 270860	NA (NA)	40 (0.890)	32/296	0.5	100%	noncoding (320/1221 nt)	IS30	repeat region
	?	CP009273	299113 =	0 (0.000)					coding (412/615 nt)	yagU	DUF1440 family inner membrane protein

AAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/269813‑269666 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTGGAAATACTTCAGCGACCACACAATAACCGACAGCAAACACTATCGAAAAGATAATGTGCGTAACACCAACCCAGTTAAAGACATGCCCGGCAAAGGTATAAAC  <  CP009273/299116‑298972                                                                                                                                                                                                                                                                                                        AAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGACGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCC                                                                                                                                                  <  2:721841/149‑1 AAAAACTGGGGATAACGCCTTAAAGGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATAAACAGAAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCC                                                                                                                                                  <  2:441478/149‑1  AAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGGGTGAGCCGA‑AAAGAATTTCCCCGCTTATTCGACCCTTCCCg                                                                                                                                                 >  1:216771/1‑148  AAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCT                                                                                                                                                 >  2:221205/1‑149   AAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGCGAAAAAACCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGC‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTA                                                                                                                                                >  1:82984/1‑149    AACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCGGA‑GAAGAATTTCCCCGCTAATTCGACACTTCCCTAA                                                                                                                                               >  2:287851/1‑149    AACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAAGTTCCCCGCTTATTCGCACCTTCCCTAA                                                                                                                                               >  1:513482/1‑149     accactGATCACGCCTTAAACGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGCAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAA                                                                                                                                              <  2:364587/143‑1      CTGGGGATAACGGCTTAAATGGCGAAGAAACGGTCTAAATGGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAG                                                                                                                                             <  2:137562/149‑1      CTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTACCTAAAG                                                                                                                                             >  2:484025/1‑149      CTGGGGATAACGCCTTAAAGGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAG                                                                                                                                             <  2:577436/149‑1         GGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAACGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCCTATCCGCACCTTCCCTAAAGCAC                                                                                                                                          <  2:501169/149‑1             TAATGCCTTAAATGGCGAAGAAACGGTCCAAATAGGCTGAGTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGC                                                                                                                                      <  2:100146/149‑1              AACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGGGAAAAAATCGGCTCAAACATGAGGAAATGAAATGACGGAGTCAGCCGA‑AAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCtgcgtcg                                                                                                                                     >  2:323515/1‑142               ACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTAAGACGA‑GAAGAATTTCACCGCGTATTCGCACCTTCCCTCAAGgcctaggtg                                                                                                                                    >  2:99713/1‑140                 GCCTTAAAGTGAGAAGAAACGGACTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGT                                                                                                                                  <  1:438928/149‑1                   CTTAAAAGGCGAAGAATCGGTCTAAACCGGCTGATTCAAGGAAAAAAAAAAAAAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAA                                                                                                                                <  2:493805/149‑1                    TTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGGAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAG                                                                                                                               >  1:693750/1‑149                             GAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGT‑TGTTGATTCAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCCCCTGCCAGTAAGCCCTGCCAG                                                                                                                      >  1:171291/1‑149                              cAGAAACGGTCTAAATTGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGA                                                                                                                     <  1:230639/148‑1                                 AAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAAACGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTT                                                                                                                  >  2:529646/1‑149                                   ACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTA                                                                                                                >  2:382539/1‑149                                    CGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGACGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAA                                                                                                               >  1:702489/1‑149                                     GGTCTAAATAGGCTGATTCAAGGCATTTACAGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCACCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAAT                                                                                                              >  1:577361/1‑149                                         TAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTT                                                                                                          <  1:444867/149‑1                                         TAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCAGCCAGAGTTTAATTTTT                                                                                                          >  2:572903/1‑149                                             TAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTGGAA                                                                                                      <  2:562937/149‑1                                             TAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTGGAA                                                                                                      <  2:562912/149‑1                                                 CTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTCGAAATAg                                                                                                  >  1:673194/1‑148                                                      TCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGGGTTTAATTTTTGGAAATCCTTCAG                                                                                             >  1:424779/1‑149                                                        AAGGCATTTACGGGAGAAAAAATCGGCTGAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTGGAAATACTTCAGCG                                                                                           >  1:444122/1‑149                                                        AAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTTGAAATACTTCAGCG                                                                                           >  2:556969/1‑149                                                           GCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTGGAAATACTTCAGCGACC                                                                                        <  1:252625/149‑1                                                                     GAGAAAAAATCGGCTCAAGCGTGAAGTAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTGGAAATACTTCAGCGACCACACAATAAC                                                                              <  2:93318/149‑1                                                                        AAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTGGAAATACTTCAGCGACCACACAATAACCGA                                                                           >  1:141806/1‑149                                                                          AAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCAGCCAGCAAGCATTGCCTGATTTTAATTTTTGGAAATACTTCAGCGACCACACAATAACCGACA                                                                         <  1:725605/149‑1                                                                                      AACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTGGAAATACTTCAGCGACCACACAATAACCGACAGCAAACACTATC                                                             >  1:614955/1‑149                                                                                           GAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTGGAAATACTTCAGCGACCACACAATAACCGACAGCAAACACTATCGAAAA                                                        <  1:382539/149‑1                                                                                           GAAGAAATGAAATGACCGAGTCAGCCGA‑GAAGACTTTCCCCGCCTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATCTTTGGAAATACTTCAGCGACCACACAATAACCGACAGCAAACACTATCGAAAA                                                        <  2:54077/149‑1                                                                                            AAGAAATGAAATGACTGAGTCAGCCGC‑GGGGCAGTGGCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTGGAAATCCTTCAGCGACCACACAATAACCGACAGCAAACACTGTCGACAAG                                                       >  1:360310/1‑149                                                                                               AAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTGGAAATACTTCAGCTACCACACAATAACCGACGGCAAACACTATCGAAAAGATA                                                    >  2:378384/1‑149                                                                                                     aaacACTGAGCCACCCGA‑GAAGAAGTCCCCCGCCTATCCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTGGAAATACTTCAGCGACCACACAATAACCGACAGCAAACACTATCGAAAAGATAATGTGC                                              <  1:63531/145‑1                                                                                                      ATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTCATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTGGAAATACTTCAGCGACCACACAATAACCGACAGCAAACACTATCGAAAAGATAATGTGCG                                             >  1:472170/1‑149                                                                                                      ATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTAATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTGGAAATACTTCAGCGACCACACAATAACCGACAGCAAACACTATCGAAAAGATAATGTGCG                                             >  2:708834/1‑149                                                                                                                 AGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTACGCCATCCCAGAGTTAAATCTTTGGAAATACTACAGCGACCACACAATAACCGACAGCAAACACTATCGAAAAGATAATGTGCGTACACCCAACC                                  >  2:42274/1‑149                                                                                                                             ATTTCCCCGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTGGAAATACTTCAGCGACCACACAATAACCGACAGCAAACACTATCGAAAAGATAATGTGCGTAACACCAACCCAGTTAAAGAC                       <  2:171291/149‑1                                                                                                                                TCCCAGCTTATTCGCACCTTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTGGAAATACTTCAGCGACCACACAATAACCGACAGCAAACACTATCGAAAAGATAATGTGCGTAACACCAACCCAGTTAAAGACATG                    >  2:375006/1‑149                                                                                                                                    CGCTTATCCGCACCTTCCCTAAAGCACCTACCAGTAAGCCCTGCCAGAGTTAAATTTTTGGAAATACTTCAGCGACCACACAATAACCGACAGCAAACACTATCGAAAAGATAATGTGCGTAACACCAACCCAGTTAAAGACATGCCCG                <  2:464099/149‑1                                                                                                                                                  TTCCCTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTGGAAATACTTCAGCGACCACACAATAACCGACAGCAAACACTATCGAAAAGATAATGTGCGTAACACCAACCCCGTTAGAGGCATGGCCGGCAAAGGTATAGAC  >  1:257431/1‑149                                                                                                                                                                                                                                                                                                        AAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGA‑GAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/269813‑269666 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAAAGCACCTGCCAGTAAGCCCTGCCAGAGTTTAATTTTTGGAAATACTTCAGCGACCACACAATAACCGACAGCAAACACTATCGAAAAGATAATGTGCGTAACACCAACCCAGTTAAAGACATGCCCGGCAAAGGTATAAAC  <  CP009273/299116‑298972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.