| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,985,522 | T→C | 17.5% | L337P (CTC→CCC) | cyaA → | adenylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,985,522 | 0 | T | C | 17.5% | 35.3 / 3.0 | 23 | L337P (CTC→CCC) | cyaA | adenylate cyclase |
| Reads supporting (aligned to +/- strand): ref base T (5/14); new base C (2/2); total (7/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.10e-01 | |||||||||||
GCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGTAGGCTG‑GCGTCGCGCAGTGTTGAGCCAGTTAGTGAGCGAGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGT > CP009273/3985379‑3985668 | gCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGcc > 1:99083/1‑149 (MQ=255) aTTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGGCGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTg < 1:6282/149‑1 (MQ=255) ggaTCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGt < 2:311097/147‑1 (MQ=255) tctcGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGCTTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGGGAAAAGCCCAGCCGTGAACGCGCCTGCGTAGGCTG‑GCGTc > 1:265496/1‑149 (MQ=255) tctcGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCCCAGCCGTGAACGCGCCTGCGTAGGCTG‑GCGTc > 1:73418/1‑149 (MQ=255) ctcGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGTAGGCTG‑GCGTcg > 2:245023/1‑149 (MQ=255) tcGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGTAGGCTG‑GCGTcgc < 2:114765/149‑1 (MQ=255) aTCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGTAGGCTG‑GCGTCGCGCa < 1:300099/149‑1 (MQ=255) ccATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGTAGGCTG‑GCGTCGCGCAgt < 1:128139/149‑1 (MQ=255) tgCTGGAGCGTGTTACTGAATTCCTGACGGCGATTGAAGATTTTTCCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGTAGGCTG‑GCGTCGCGCAGTGTTGAGCCAGTTa < 1:9211/149‑1 (MQ=255) tgCTGGAGCGTGTTACTGAATCCCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGTAGGCTG‑GCGTCGCGCAGTGTTGAGCCAGTTa < 2:31768/149‑1 (MQ=255) atggtcggtgtTACTGAATACCGGACGGCGATTGAAGATTTTACCGGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGTAGGCTG‑GCGGCGCGCAGTGTTGAGCCAGTTAGt < 1:109524/142‑1 (MQ=255) aGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGAGCATACTTAGCCTT‑GCGTCGCGCAGTGTTGAGCCAGTTAGTGAGc < 2:75239/149‑1 (MQ=255) tGACGGCGATTGAAGATTTTACCGGTCTGGATTTAGTACGTCGCTGCTTCTATTTCAACGTGTGCGAAAAGCTCAGCCGTGACCGCGCCTGCGTAGGCTG‑GCGTCGCGCAGTGTTGAGCCAGTTAGTGAGCGAGTGGGGTTGGGACGaa < 1:264904/149‑1 (MQ=255) gATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGTAGGCTG‑GCGTCGCGCAGTGTTGAGCCAGTTAGTGAGCGAGTGGGGTTGGGACGAAGCTCGTc > 2:18874/1‑149 (MQ=255) tttACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGTAGGCTG‑GCGTCGCGCAGTGTTGAGCCAGTTAGTGAGCGAGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCt < 2:179793/149‑1 (MQ=255) tACCCGTCTGGATTTAGTCCGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGGCGGCTG‑GCGTCGCGCAGTGTTGAGCCAGATAGTGAGCGAGGGGGGATGGGACGAAGCTCGTCTGGCAATGCtac > 1:264156/1‑147 (MQ=255) tGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGTAGGCTG‑GCGTCGCGCAGTGTTGAGCCAGTTAGTGAGCGAGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACcgc > 2:282233/1‑149 (MQ=255) tctatgtccaaGCGTGCGACAAGCTCCGCCGTGCACGCGCCTGCGTCGGCTGCGCG‑CGCGCAGGGTATAGCCAGTCACTGAGAGAGTGTGGCCCTCTCCGAGCCCCTCTCGATATGCTCGATAACCGCGCTAACTGGAAGATTGATCAg < 1:91446/140‑1 (MQ=255) aaaaGTGTGCGAAAAGCCCAGCCGTGAACGCGCCTGCGTAGGCTG‑GCGTCGCGCAGTGTTGAGCCAGTTAGTGAGCGAGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTg < 2:265496/149‑1 (MQ=255) aaaaGTGTGCGAAAAGCCCAGCCGTGAACGCGCCTGCGTAGGCTG‑GCGTCGCGCAGTGTTGAGCCAGTTAGTGAGCGAGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTg < 2:73418/149‑1 (MQ=255) gCGAAAAGCTCAGCCGTGAACGCGCCTGCGTAGGCTG‑GCGTCGCGCAGTGTTGAGCCAGTTAGTGAGCGAGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGcac < 2:99083/149‑1 (MQ=255) gCTCAGCCGTGAACGCGCCTGCGTAGGCTG‑GCGTCGCGCAGTGTTGAGCCAGTTAGTGAGCGAGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGt < 2:143756/149‑1 (MQ=255) | GCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGTAGGCTG‑GCGTCGCGCAGTGTTGAGCCAGTTAGTGAGCGAGTGGGGTTGGGACGAAGCTCGTCTGGCAATGCTCGATAACCGCGCTAACTGGAAGATTGATCAGGTGCGTGAGGCGCACAACGAGT > CP009273/3985379‑3985668 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |