Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	1,191,733	C→T	N385N (AAC→AAT)	icd →	e14 prophage; isocitrate dehydrogenase, specific for NADP+

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,191,733	0	C	T	100.0%	40.1 / NA	22	N385N (AAC→AAT)	icd	e14 prophage; isocitrate dehydrogenase, specific for NADP+
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/9);  total (13/9)

GCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGG‑CTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGA  >  CP009273/1191587‑1191798                                                                                                                                                    |                                                                  gCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc                                                                 <  1:731164/149‑1 (MQ=25)    acccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaa                                                              >  2:543614/1‑149 (MQ=21)           gTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGGTAAAGGTATGGGAGGCGCAATCAATGCCAAGACCGTa                                                       >  1:273059/1‑149 (MQ=18)                gcgcCGAAAGAGGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCGGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTa                                                  <  2:46792/149‑1 (MQ=17)                 cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGGAGGCGCAATCAGTGCCAAGAACGTAActttt                                                 >  1:171861/1‑145 (MQ=18)                 cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACCGACGAGGAATGGC‑TGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt                                                 <  1:506064/149‑1 (MQ=18)                   ccGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCCCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTTACTTGAATGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGa                                               <  1:31977/149‑1 (MQ=18)                    cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGGTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGAc                                              >  1:729438/1‑149 (MQ=17)                      aaaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt                                            >  1:14217/1‑149 (MQ=17)                      aaaTAGGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt                                            <  2:41421/149‑1 (MQ=17)                            gCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACg                                      >  1:813580/1‑149 (MQ=14)                              cGGTCAGGACAAAGTAAATCCTGGCTCTTTTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTc                                    <  2:16573/149‑1 (MQ=14)                              cGGGCAGGACATAGTGAATCCTGGCTCTTTTATGCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTc                                    <  2:468857/149‑1 (MQ=11)                                gTCAGGACAAAGTAAATCCTGTCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTg                                  >  2:801840/1‑149 (MQ=14)                                gTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGGGATGATGCTGCGCCATATGGGTTGGACTGAATCGG‑CTGGCCTGGCTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTg                                  <  2:751202/149‑1 (MQ=14)                                     gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgatgga                             >  2:16514/1‑149 (MQ=14)                                      aCAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgatggga                            >  1:400441/1‑147 (MQ=11)                                                     gCTCTATTATTCTCGCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGctgctg             <  2:544749/149‑1 (MQ=14)                                                         tattatTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCATGACCGTAACTTATGACTTTGAACGCCTGATGGAAGGCGCTAAGCTGCTGAAAt         <  1:525884/149‑1 (MQ=14)                                                         tattatTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAAt         >  1:927107/1‑149 (MQ=14)                                                         tattatTCTCTCAGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAAt         >  2:800875/1‑149 (MQ=14)                                                          attattCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACAGTAACTTATGAATTCGAAGGTCTGATGGAAGGCCCTAAGCTGCTGAAATg        >  2:849119/1‑149 (MQ=14)                                                                ctctcCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGG‑CTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGACGCTAAGCTGCTGAACTGTTCaga  >  2:120041/1‑149 (MQ=14)                                                                                                                                                    |                                                                  GCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGG‑CTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGA  >  CP009273/1191587‑1191798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑