Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	1,449,090	C→A	Q303K (CAA→AAA)	paaA →	ring 1,2‑phenylacetyl‑CoA epoxidase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,449,090	0	C	A	100.0%	73.1 / NA	29	Q303K (CAA→AAA)	paaA	ring 1,2‑phenylacetyl‑CoA epoxidase subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/14);  total (15/14)

TGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGGAGAGGCTCGACGCCAAACGTAAAGCCTGGGAAGAAGGTACCTGGGTACGGGAAGCAGCGCTGGCCCATGCAC‑AAAAACAACATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGTTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAA  >  CP009273/1448951‑1449235                                                                                                                                             |                                                                                                                                                  tGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGGAGAGGCTCGACGCCAAACGTAAAGCCTGGGAAGAAGGTACCTGGGTACGGGAAGCAGCGCTGGCCCATGCAC‑AAAAAAAACAAGccc                                                                                                                                          >  2:449234/1‑149 (MQ=255)    tGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGGAGAGGCTCGACGCCAAACGTAAAGCCTGGGAAGAAGGTACCTGGGTACGGGAAGCAGCGCTGGCCCATGCAC‑AAAAAAAACATGCCCGTa                                                                                                                                       <  2:137300/149‑1 (MQ=255)     gagaTCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGGAGAGGCTCGACGCCAAACGTAAAGCCTGGGAAGAAGGTACCTGGGTACGGGAAGCAGCGCTGGCCCATGCAC‑AAAAAAAACATGCCCGTaa                                                                                                                                      >  2:86212/1‑149 (MQ=255)          cGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGGGGAGAATCCACGCCAAACGTAAAGCCAGGGAAGAAGGTACCCGGCTACGGGAAGCAGCGCGGGCCCATCCAC‑AAAAAAAACATGCCCgcgaaggcg                                                                                                                                 >  2:644990/1‑141 (MQ=255)          cGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGGAGCGGCTCGACGCCAAACGTAAAGCCTGGGAAGAAGGTACCTGGGTACGGGAAGCAGCGCTGGCCCATGCAC‑AAAAAAAACATGCCCGTAAGGTCg                                                                                                                                 >  2:351695/1‑149 (MQ=255)                  aGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGGAGAGGCTCGACGCCAAACGTAAAGCCTGGGAAGAAGGTACCTGGGTACGGGAAGCAGCGCTGGCCCATGCAC‑AAAAAAAACATGCCCGTAAGGTCGCATAAGga                                                                                                                         >  1:528483/1‑149 (MQ=255)                        ttAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGGAGAGGCTCGACGCCAAACGTAAAGCCTGGGAAGAAGATACCTGGGTACGGGAAGCAGCGCTGGCCCATGCAC‑AAAAAAAACATGCCCGTAAGGTCGCATAAGGCGATGCa                                                                                                                   >  1:687994/1‑149 (MQ=255)                         tAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGGAGAGGCTCGACGCCAAACGTAAAGCCTGGGAAGAAGGTACCTGGGTACGGGAAGCAGCGCTGGCCCATGCAC‑AAAAAAAACATGCCCGTAAGGTCGCCTAAGGAGATTCaa                                                                                                                  >  2:169463/1‑149 (MQ=255)                              aaGTAATTAACGGTCGCGGAATTTGTAATCAGGAGAGGCTCGACGCCAAACGTAAAGCCTGGGAAGAAGGTACCTGGGTACGGGAAGCAGCGCTGGCCCATGCAC‑AAAAAAAACATGCCCGTAAGGTCGCATAAGGCGGTTCAAAATGa                                                                                                             >  2:170594/1‑149 (MQ=255)                                    ttAACGGGCGCGGAATTTGTAATCAGGAGAGGCTAGACGCCAAACGTAAAGCCGGGGAAGAAGGTACCTGGGTAAGGGAAGCAGCGCTGGCCCATGCCCAAAAAAAAACATGCCCGAAAGGTCGGATCAGGAGATTCcaacagaggaaa                                                                                                        >  2:539543/1‑140 (MQ=255)                                                gAATTGGTAATCAGGAGAGGCTCGACGCCAAACGTAAAGCCTGGGAAGAAGGTACCTGGGTACGGGAAGCAGCGCTGGCCCATGCAC‑AAAAAAAACATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGt                                                                                           <  2:586954/149‑1 (MQ=255)                                                       tAATCAGGAGAGGCTCGACGCCAAACGTAAAGCCTGGGAAGAAGGTACCTGGGTACCGGAAGCAGCGCTGGCCCATGCAC‑AAAAAAAACATGCCCGTAAGGTCGCATAAGGGGGTTCAAAATGAGTAAGGTTTAATGGCCGTTATACGa                                                                                    >  1:526813/1‑149 (MQ=255)                                                        aaTCAGGAGAGGCCCGACGCCAAACGTAAAGCCTGGGAAGAAGGTACCTGGGAACGGGAAGCAGCGCTGGCCCATGCAC‑AAAAAAAACATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGaa                                                                                   <  2:241616/149‑1 (MQ=255)                                                          tCAGGAGAGGCTCGACGCCAAACGTAAAGCCTGGGAAGAAGGTACCTGGGTACGGGAAGCAGCGCTGGCCCATGCAC‑AAAAAAAACATGCCCGTAAGGGCGCATAAGGAGATTCAAAATGAGTAATGTTGACTGGCCGGTATACGAAGt                                                                                 >  1:703935/1‑149 (MQ=255)                                                            aGGAGAGGCTCGACGCGCAACGTAAAGGCTGGAAAGAAGGTACCCGGGTACGGGAAGCAGCGCTGGCCCATGCAC‑AAAAAAAACATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGttt                                                                               <  1:719455/149‑1 (MQ=255)                                                                gagGCTCGACGCCAAACGTAGAGCCTGGGAAGAAGGTACCTGGGTACGGGAAGCAGCGCTGGCCCATGCAC‑AAAAAAAACATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTcgt                                                                           <  1:612987/149‑1 (MQ=255)                                                                      cGACGCCAAACGGAAAGCCTGGGAAGAAGGTACCTGGGTACGGGAAGCAGCGCTGGCCAATGCAC‑AAAAAAAACATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTgg                                                                     <  1:84431/149‑1 (MQ=255)                                                                                    aaGCCTGGGAAGAAGGTAACTGGGTACGGGAAGCAGCGCTGGCCCATGCAC‑AAAAAAAGCATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTAt                                                       <  1:351695/149‑1 (MQ=255)                                                                                     aGCCTGGGAAGAAGGTACCTGGGTATGGGAAGCGCCGCTGGGCCATTCAC‑AAAAAAAACATGCCAGTAAGGTCGCATAAGGAGATTCAAAATGCGCAATGTTTACTGGCCGTTATACGAAGTTATCGTGCGTAGCCAGAAGGACTTaac                                                      >  2:155078/1‑147 (MQ=255)                                                                                        cTGGGAAGAAGGTACCTGGGTACGGTAAGCAGCGCTGGCCCATGCAC‑AAAAAAAACATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCTGTTACACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATcaca                                                   <  2:493468/149‑1 (MQ=255)                                                                                         tGGGAAGAAGGTACCTGGGTACGGGAAGCAGCGCTGGCGCATGCAC‑AAAAAAAACATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAGCAGGGGTTATCacac                                                  >  2:416630/1‑149 (MQ=255)                                                                                            gaagaaGGTACCTGGTCACGGGAAGCAGCGCTGGCCCTTGCAC‑AAAAAAAACATGGCCGTAAGGTCGCATAAGGAGATTTAAAATGACTATTGTTTACTGGCCGTTATACGAAGTTTTCGTGCGGGGCAAACAGGGCTTATCACACCGc                                               <  1:444154/149‑1 (MQ=255)                                                                                                           gTACGGGAAGCAGCGCTGGCCCATGCAC‑AAAAAAAACATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCCTGTTGGCAGTTTa                                <  2:528483/149‑1 (MQ=255)                                                                                                              cGGGAAGCAGCGCTGGCCCATGCAC‑AAAAAAAACATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATTACACCGCCATGTTGGCAGTTTACAt                             <  2:463186/149‑1 (MQ=255)                                                                                                                  aaGCAGCGCTGGCCTATGCAC‑AAAAAAAACATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGTTGGCAGTTTACAtgctg                         <  2:669809/149‑1 (MQ=255)                                                                                                                       gcgcTGGCCCATGCAC‑AAAAAAAACATGCCCGTAAGGTCGCGTAAGGAGATTCAAAATGAGAAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTCGCGAACAGAGCGTAGCACCCCGCCTTGTTGGCAGGGTACGTGCTGCCggt                    >  1:209825/1‑147 (MQ=255)                                                                                                                         gcTGGCCCATGCAC‑AAAAAAAACATGCCCGAAAGGTCGCATAAGGCGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGTTGGCAGTTTACATGCTGCCGATGa                  <  1:492439/149‑1 (MQ=255)                                                                                                                               ccATGCAC‑AAAAAAAACGTGCCCGTAAGGTCGCATAAGGCGAGTAAAAATGAGTAATATTTACTGGGCGTTATCCGAAGTTTTCGTGCGTAGAAAACAGGGCTTATCACACCGCCATGTTGGGAGGTTACATGCTGCGGATGCGCCGag            >  1:204810/1‑148 (MQ=255)                                                                                                                               ccATGCAC‑AAAAAAAACATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGTTGGCAGTTTACATGCTGCCGATGAGCGGAt            <  1:449234/149‑1 (MQ=255)                                                                                                                                      aaaaaaaaaaCATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGTTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACt      <  1:169463/147‑1 (MQ=255)                                                                                                                                        aaaaaaaaCATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACGGCCATGTTGGCAGTTTAGATGCTGCCGATGAGCGGATGGCACggg    >  1:651304/1‑149 (MQ=255)                                                                                                                                          aaaaaaCATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGTTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGaa  >  1:660031/1‑149 (MQ=255)                                                                                                                                             |                                                                                                                                                  TGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGGAGAGGCTCGACGCCAAACGTAAAGCCTGGGAAGAAGGTACCTGGGTACGGGAAGCAGCGCTGGCCCATGCAC‑AAAAACAACATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGTTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAA  >  CP009273/1448951‑1449235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑