Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,953,437 Δ1 bp 67.6% pseudogene (178/243 nt) yifN ← PemK toxin family pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,953,4370C.67.6% 46.2 / 56.1 37pseudogene (178/243 nt)yifNPemK toxin family pseudogene
Reads supporting (aligned to +/- strand):  ref base C (4/7);  new base . (15/10);  total (20/17)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.32e-01

GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACTCATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTA  >  CP009273/3953300‑3953580
                                                                                                                                         |                                                                                                                                               
gggcccTCAAGGGGAGAAGACTTAGGGCCTCTATGGACTACAAAGTCATTATCGGGGATGGTTATTTTATCGTCAATTTATGAGCGATATGGCATTGACTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACTCAtgtg                                                                                                                                            <  2:25293/140‑1 (MQ=255)
gggcccTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACTCAtgtg                                                                                                                                            <  2:48302/140‑1 (MQ=255)
   cccTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACT‑ATGTGGCaa                                                                                                                                        <  2:250306/143‑1 (MQ=255)
         aGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACTCATGTGGCAAACATTGATTaa                                                                                                                             <  1:189361/149‑1 (MQ=255)
              agaAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTCTTTTATTGACAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCCAC‑GATCTGGCAAACAATGATTaa                                                                                                                             >  2:74374/1‑143 (MQ=255)
                aaaaCTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGa                                                                                                                           >  2:395171/1‑143 (MQ=255)
                   aCTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATAc                                                                                                                        >  2:467127/1‑143 (MQ=255)
                     ttAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATAccc                                                                                                                      >  2:244907/1‑143 (MQ=255)
                                aTGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACTCATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGct                                                                                                      <  1:116206/149‑1 (MQ=255)
                                      tACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTa                                                                                               >  1:303515/1‑149 (MQ=255)
                                           aGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCGGTAAGTCCTTGCGCTGTTTAATCTg                                                                                          >  1:465568/1‑149 (MQ=255)
                                              cATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCAGTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAAt                                                                                             <  2:101849/143‑1 (MQ=255)
                                                  aTCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGt                                                                                         <  2:353884/143‑1 (MQ=255)
                                                           ggTTATTTTCTTGCCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACttgtt                                                                          <  1:257807/149‑1 (MQ=255)
                                                                        tCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACTCATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCa                                                                    <  2:351500/143‑1 (MQ=255)
                                                                             ttATGAGGGATATGGCATTTATTGACTTGATGACCGCCCTCTGTATGTCCGCAACCAACT‑ATGTGGCCAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTgg                                                        <  1:452739/149‑1 (MQ=255)
                                                                              tATGAGCGATACGGCAGTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACTCATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCgg                                                              <  2:49881/143‑1 (MQ=255)
                                                                                  aGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCaa                                                   >  1:390892/1‑149 (MQ=255)
                                                                                    cGATATGGCATTGATTGGCTTGATGACCGCCCTCTGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGc                                                       <  2:64758/143‑1 (MQ=255)
                                                                                    cGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGc                                                       >  2:421822/1‑143 (MQ=255)
                                                                                           gCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACTCATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACg                                                 >  2:13799/1‑143 (MQ=255)
                                                                                               tgattgaCTTGATGACCGCCCTCTGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCGTTGGCCCAACGAATct                                            >  2:441664/1‑143 (MQ=255)
                                                                                                        tgattaCCGCCCTCTTTATGTCTGCAACCAACT‑ACGTGGCAAACATGGATTCAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCTGCTTTTGCCCAACGAATCTGCTGATCaa                                   <  2:475023/143‑1 (MQ=255)
                                                                                                         gatgaCCGCTCTCTGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATTCCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCaaa                                  <  2:146878/143‑1 (MQ=255)
                                                                                                         gatgaCCGCCCTCTGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTg                            >  1:339005/1‑149 (MQ=255)
                                                                                                                    tctGTATGTCTGCAACCAACTCATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAgg                        >  2:108885/1‑143 (MQ=255)
                                                                                                                    tctGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGa                 >  1:357496/1‑149 (MQ=255)
                                                                                                                    tctGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGa                 >  1:235711/1‑149 (MQ=255)
                                                                                                                      tGTATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCGGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATa               >  1:127034/1‑149 (MQ=255)
                                                                                                                        tATGTCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAAc             <  1:187917/149‑1 (MQ=255)
                                                                                                                         aTGTCTGCAACCAACTCATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCCTGCTTCAATTTGTCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAAc             <  1:518911/149‑1 (MQ=255)
                                                                                                                            tCTGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCGGGTCATTAATAACATTa         >  1:140169/1‑149 (MQ=255)
                                                                                                                              tGCAACCAACTCATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATaa              >  2:284577/1‑143 (MQ=255)
                                                                                                                              tGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGCTCAAAGAATTGCAGGTCATTGATAACATTACt       >  1:19326/1‑149 (MQ=255)
                                                                                                                              tGCAACCAACT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACt       <  1:439466/149‑1 (MQ=255)
                                                                                                                                  accaacTCATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCCCTGTTTAATCTGCTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACAtt          >  2:53450/1‑143 (MQ=255)
                                                                                                                                   ccaacT‑ATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTa  >  1:466515/1‑149 (MQ=255)
                                                                                                                                         |                                                                                                                                               
GGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAACTCATGTGGCAAACATTGATTAAGATACCCTCTGTAAGTCCTTGCTCTGTTTAATCTGTTTCGACTGACTTGTTGTACCAGGTCGGCTTTGGCCCAACGAATCTGCTGATCAAAGAATTGCAGGTCATTGATAACATTACTGGCTA  >  CP009273/3953300‑3953580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑