Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	741,050	C→A	L144M (CTG→ATG)	ybgL →	UPF0271 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	741,050	0	C	A	98.3%	191.6 / NA	59	L144M (CTG→ATG)	ybgL	UPF0271 family protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (27/31);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGGCGCA  >  CP009273/740910‑741189                                                                                                                                             |                                                                                                                                             gcggcgTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGa                                                                                                                                           >  1:73032/1‑144 (MQ=255) gcggcgTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGt                                                                                                                                      <  2:783497/149‑1 (MQ=255) gcggcgTAAGGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGa                                                                                                                                           <  1:61106/144‑1 (MQ=255)    gcgTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCCTCGCCAGAGCGGTAGACGCGTGCGATCCAGCCTTGATTCTCGTCGGGCTGGCGGGGCGCGAGCTGGTTc                                                                                                                                        >  1:807638/1‑144 (MQ=255)       tAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTg                                                                                                                                     <  1:182464/144‑1 (MQ=255)       tAATGCGGCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTg                                                                                                                                     <  1:418035/144‑1 (MQ=255)           gCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAgg                                                                                                                                 >  1:634508/1‑144 (MQ=255)           gCGTCATGACAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGgcaagc                                                                                                                            <  2:829948/149‑1 (MQ=255)                aTGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGgcaagc                                                                                                                            >  1:641194/1‑144 (MQ=255)                  gTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATgg                                                                                                                     >  2:551553/1‑149 (MQ=255)                   tCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGGCGGGCTGCCGGGAGAAGAGATGATTCGTGCAGGCAAGCAATATGGt                                                                                                                    >  2:138985/1‑149 (MQ=255)                     caaCCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCt                                                                                                                  <  2:305875/148‑1 (MQ=255)                        ccGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGt                                                                                                                    >  1:13327/1‑144 (MQ=255)                            aCGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGa                                                                                                                <  1:835135/144‑1 (MQ=255)                             cGGCATGTTGTACAACCAGGCGGCGAAAGACGCCCAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AAcgc                                                                                                         <  2:448786/149‑1 (MQ=255)                               gCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATAGGGTCTGAC‑AAcgcgc                                                                                                       >  2:175186/1‑149 (MQ=255)                                cATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATGTGGTCTGGC‑acc                                                                                                           >  1:890651/1‑142 (MQ=255)                                cATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AAc                                                                                                           >  1:912465/1‑144 (MQ=255)                                        aCAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGGCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAgtg                                                                                              <  2:298511/149‑1 (MQ=255)                                          aaCCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGtt                                                                                            <  2:190421/149‑1 (MQ=255)                                          aaCCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGtt                                                                                            <  2:870944/149‑1 (MQ=255)                                           aCCAGGCGGCGAAAGAAGCACAACTGGTCGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGGCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGttt                                                                                           <  2:882834/149‑1 (MQ=255)                                            ccAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAgt                                                                                               <  1:720870/144‑1 (MQ=255)                                             cAGGCGGCGAAAGAAGCCCAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGc                                                                                         <  2:1013348/149‑1 (MQ=255)                                             cAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGc                                                                                         <  2:690903/149‑1 (MQ=255)                                                     gAAAGAAGCACAACTGGCAGACGCTATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATcgcg                                                                                 >  2:767999/1‑149 (MQ=255)                                                     gAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATcgcg                                                                                 <  2:15209/149‑1 (MQ=255)                                                       cagaagCACAACGGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGt                                                                               <  2:127809/148‑1 (MQ=255)                                                            gCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTCTCa                                                                          >  2:596826/1‑149 (MQ=255)                                                             cacaACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGtt                                                                              >  1:911589/1‑144 (MQ=255)                                                                   tGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑CACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCggg                                                                        >  1:589333/1‑144 (MQ=255)                                                                   gggCAGACGCCACCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGACGAAAGC‑CTGGGGAGTGTTTGCCGATCGCGGTTATCAGGCTGAc                                                                   <  2:249576/148‑1 (MQ=255)                                                                      cAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGGCGATCGCGGTTATCAGGCTggcggc                                                                >  2:758818/1‑149 (MQ=255)                                                                            ccATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCt                                                               >  1:425224/1‑144 (MQ=255)                                                                              aTCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGt                                                        <  2:897637/149‑1 (MQ=255)                                                                              aTCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGt                                                        <  2:231230/149‑1 (MQ=255)                                                                                cGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCt                                                           <  1:166860/144‑1 (MQ=255)                                                                                      agCGGTATACGTTTGCGATCCAGCATTGATTCTCGTCGGGTTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGaa                                                <  2:725331/149‑1 (MQ=255)                                                                                        cGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGc                                              <  2:594958/149‑1 (MQ=255)                                                                                        cGGTATACGCTTGCGAGCCAGCATTGATTCTCGTCGGGCGGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCcgc                                                   <  1:172135/144‑1 (MQ=255)                                                                                            ataCGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGt                                          <  2:862437/149‑1 (MQ=255)                                                                                                 cTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGgc                                     <  2:474074/149‑1 (MQ=255)                                                                                                  ttGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGgcg                                    >  2:455848/1‑149 (MQ=255)                                                                                                  ttGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGgcg                                    >  2:867908/1‑149 (MQ=255)                                                                                                   tGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCCGGCTGACGGCTCGCTGGTGCCGCGCAGCCAGTCa                                        >  1:726485/1‑144 (MQ=255)                                                                                                    gcgcTCCTGCATTGATTCTCGTCGTGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAg                                       <  1:594942/144‑1 (MQ=255)                                                                                                           aGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGGGAAGCCAGGCCGGCGCGttgattg                           >  2:174346/1‑149 (MQ=255)                                                                                                           aGCATTGATTCTCGCCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGtt                                <  1:333197/144‑1 (MQ=255)                                                                                                               ttGATTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGaaaa                       <  2:214802/149‑1 (MQ=255)                                                                                                                  aTTCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGaa                         >  1:358580/1‑144 (MQ=255)                                                                                                                   ttCTCGTCGGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACgaa                   >  2:1013373/1‑149 (MQ=255)                                                                                                                        gtcgGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCATGCGCGTTGATTGAAAACGAAGAACa              <  2:615883/149‑1 (MQ=255)                                                                                                                         tcgGGCTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGAGCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGACTGAAAACgaag                  >  1:590662/1‑144 (MQ=255)                                                                                                                            ggCTGGCGGGAAGCGAGATGATTAGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGgcg          >  2:566472/1‑149 (MQ=255)                                                                                                                              cTGGCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGCTGATTGAAAACGAAGAACAGGCGCt        >  2:878481/1‑149 (MQ=255)                                                                                                                                 gCGGGAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑ACCGCAAGATTAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGgc     <  2:706166/149‑1 (MQ=255)                                                                                                                                   gggAAGCGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGGTTTAAAACGAAGAACATGCGCTGgcgc   >  2:864030/1‑149 (MQ=255)                                                                                                                                    ggAAGCGAGATGATTCGTGCAGGCAAGCAATATGATCTGAC‑ACCGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGCTTGAAAACGAAGAACAGGCGCCGGCGCa  >  2:916589/1‑149 (MQ=255)                                                                                                                                         cGAGATGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGGCGCa  >  1:702904/1‑144 (MQ=255)                                                                                                                                             |                                                                                                                                             GCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGAC‑AACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAGGCGCTGGCGCA  >  CP009273/740910‑741189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑