| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,435,902 | G→T | 43.4% | intergenic (‑72/+75) | rpsM ← / ← rpmJ | 30S ribosomal subunit protein S13/50S ribosomal subunit protein L36 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,435,902 | 0 | G | T | 43.4% | 2.7 / 16.7 | 21 | intergenic (‑72/+75) | rpsM/rpmJ | 30S ribosomal subunit protein S13/50S ribosomal subunit protein L36 |
| Reads supporting (aligned to +/- strand): ref base G (5/7); new base T (6/3); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
CGCCATAAATCGAAGTTAATGCGATTACGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAAT‑GGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAA > CP009273/3435758‑3436044 | cGCCATAAATCGAAGTTAATGCGATTACGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATTTGTACGTTTCATGCTGAAAAGCCCGTTTTCAGGATACTCAAAT‑GGAAACGcaca < 1:291777/149‑1 (MQ=255) ttAATGCGATTACGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGAGCCACTATGCACTCCTACTATTTAATATGTACGCTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAAT‑GGAAACGCACAAACATACAAAAGAtt > 1:186785/1‑149 (MQ=255) tAATGCGATTACGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAAT‑GTAAACGCACAGACATACAAAAGATtg < 1:193922/149‑1 (MQ=255) ggCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAAT‑GGAAACTCACAGACATACAAAAGATTGGCTGGCTAATCt > 2:266788/1‑149 (MQ=255) aTGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCAGTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAAT‑GGAAACTCACAGACATACAAAAGATTGGCTGGCTAATCTAGc > 2:306468/1‑149 (MQ=255) gCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAAT‑GGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGGAAAATAAGCGaaaa > 2:280726/1‑149 (MQ=255) cACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAAT‑GGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGaaaaaa > 2:286115/1‑149 (MQ=255) aCTCCTACTATTTAATATGTACGTTCCATTCTGAAAAGCCCGTTTTCAGGATACTCAAAT‑GGAAACTCGCAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGCAAAAATCAGCCtt > 1:80262/1‑149 (MQ=255) actaTTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAAT‑GGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAGGAAAAATATGCGAAAAAAGCAACCTTGGCGCt > 1:104079/1‑149 (MQ=255) ttAATGTGTACATTCAATGATGAAAAGCCCGTTTTGAGGATATTCATAT‑GGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCATGAAAAATATAAAAAAAAATCAGCCTTGGCGCTGTTTa < 2:22998/149‑1 (MQ=255) tatGTACGTTCCATGCTGAAAAGCCCCTTTTCAGGATACTCAAAT‑GGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCt > 1:107664/1‑149 (MQ=255) acgtgccagtcggAAAAGCGCTTCTTCAGGAT‑CTCCAATGGGAAACGCAAAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGTTCAACCCAACTTTGCAAGAAAAATATTCGAAAAAATCAGCCTTGGCGCGGTTTATGCTtcggc < 2:112218/137‑1 (MQ=255) gAAAAGCCCGTTTTCAGGATACTCAAAT‑TGAAACTCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCaa > 1:89840/1‑149 (MQ=255) aaaaGCCCGTTTTCAGGATACTCAAAT‑GGAAACTCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCaaa > 2:9189/1‑149 (MQ=255) cccGTTTTCAGGATACTCAAAT‑GGAAACTCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATcaca < 1:306468/149‑1 (MQ=255) cccGTTTTCAGGATACTCAAAT‑GGAAACTCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATcaca < 1:266788/149‑1 (MQ=255) tCAGGATACTCAAAT‑GGAAACGCACAGGCATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAACTCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGa < 1:183609/149‑1 (MQ=255) tCAGCACACCCAAAT‑GGAAACGCACAGACATACAAAAGATTCGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGa < 2:117487/149‑1 (MQ=255) aTACTCAAAT‑GGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCa < 2:110151/149‑1 (MQ=255) tCAAAT‑GGAAACTCACAGACATAGAAAAGATTGGCTGGATAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCAc > 1:172723/1‑149 (MQ=255) ggAAACTCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTaa < 1:256070/149‑1 (MQ=255) | CGCCATAAATCGAAGTTAATGCGATTACGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAAT‑GGAAACGCACAGACATACAAAAGATTGGCTGGCTAATCTAGCCAGCTCAACCCAACTTTGCAAGAAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAA > CP009273/3435758‑3436044 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |