Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	CP009273	2,656,840:1	(GCGCGCA)2→3	81.2%	coding (40/804 nt)	suhB →	inositol monophosphatase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 2656826	3 (0.140)	15 (0.670) +GCGCGCA	16/228	NT	81.2%	coding (26/804 nt)	suhB	inositol monophosphatase
	?	CP009273	2656827 =	4 (0.170)					coding (27/804 nt)	suhB	inositol monophosphatase

TTTCTTCATCTAAAGATTATTCACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGA‑ACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAAC‑GATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGATTATCGACACGATTCGTAA  >  CP009273/2656687‑2656970                                                                                                                                             |                                                                                                                                                  tttcttCATCTAAAGATTATTCAGGCATGTTATCGTAAAACGAAGACAGATGCAGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGA‑ACATCGCCGTgcgcgcagc                                                                                                                                          >  2:239432‑M1/1‑140 (MQ=255)               gATTATTCACGCATCTTATCATAAAACGAAGACAGATGCGGATCTCGCTGCTATACTCTGCGCCGTTTTCCTGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGA‑TCATCGCCGTGCGCGCAGCGCGCAAgg                                                                                                                                  >  1:102379/1‑143 (MQ=255)               gATTATTCACGCATCTTATCAGAAAACGAAGACAGATGCCGATCTCGCTGCGATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGA‑ACATCGCCGTGCGCGCAGCGCGCAAGGCGGGta                                                                                                                            <  2:120146/149‑1 (MQ=255)                   attCACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGCTGATGACACACCG‑GGTgcgcgcagcgcgcagcgcgcaaggcgg                                                                                                                        <  2:212039‑M1/149‑28 (MQ=255)                                   aTAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGA‑ACATCGCCGTgcgcgcagcgcgcagcgcgcaagccgggaaatttcaatgccaa                                                                                                        >  2:110710‑M1/1‑106 (MQ=255)                                   aTAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGGTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGA‑ACATCGCCGTgcgcgcagcgcgcagcgcgcaaggcgggaactttacttgacaa                                                                                                        >  2:301344‑M1/1‑106 (MQ=255)                                    tAAAACGAAGACAGATGCCGATCTCGCTGCTATACTTTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGA‑ACATCGCCGTgcgcgcagcgcgcagcgcgcaaggcgggtaatttaatt                                                                                                             <  1:309513‑M1/143‑39 (MQ=255)                                     aaaaCGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGA‑ACATCGCCGTgcgcgcagcgcgcagcgcgcaaggcgggtaatttaattg                                                                                                            >  1:46742‑M1/1‑104 (MQ=255)                                          gAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGA‑ACATCGCCGTgcgcgcagcgagcagcgccccaggcgggtcatttcattgcccaa                                                                                                       >  1:180550‑M1/1‑99 (MQ=255)                                                   tGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGA‑ACATCGCCGTgcgcgcagcgcgcagcgcgcaaggcgggtaatttaattgccaaaaactatgaa                                                                                              <  1:123134‑M1/143‑54 (MQ=255)                                                    gCCGATCTCGCTGCTATACTCTGCGCCGTTTACCCGTTCTTTAACATCCAGTGAGCGAGACCGATGCATCCGATGCTGA‑ACATCGCCGTgcgcggagcgcgcagcgcgcaaggcgggtaagttaattgccaaaaaacttgtaa                                                                                             >  1:55445‑M1/1‑89 (MQ=255)                                                         tctcGCTGCTATACCCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGA‑ACATCGCCGTgcgcgcagcgcgcagcgcgcaaggcgggtaatttaattgccaaaaactatgaaaccccggacgct                                                                                  <  2:29685‑M1/149‑66 (MQ=255)                                                                     aCTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGA‑ACATCGCCGTgcgcgcagcgcgcagcgcgcaaggcgggtaatttaattgccaaaaactatgaaaccccggacgctgtagaa                                                                            <  1:84221‑M1/143‑72 (MQ=255)                                                                     aCTCTGCGCCGTTTTCCCGTTCTTTAACAGCCAGTGAGAGAGACCGATGCATCCGATGCTGA‑ACATCGCCGTgcgcgcagcgcgcagcgcgcaaggcgggtaatttaattgccaaaaactatgaaaccccggacgctgtagaa                                                                            <  1:125935‑M1/143‑72 (MQ=255)                                                                                      cGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGA‑ACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGCAACCCCGGACGCTGTAGAAGCGAGCCAGCAAGGCAGTAAC‑GATTTCGTg                                                    >  2:285115/1‑149 (MQ=255)                                                                                                         agagggaCCGATGCATCCGAGGCTGA‑ACATCGCCGTgcgcgcagcgcgcagcgcgcaaggcgggtaatttaatggccaaaaactatgaaaccccggacgctgtagaagcgagccagaaaggcagtaacgatttcgtgaccaacgtagat                                  <  2:60246‑M1/149‑114 (MQ=255)                                                                                                         agagagaCCGATGCATCCGATGCTGA‑ACATCGCCGTgcgcgcagcgcgcagcgcgcaaggcgggtaatttaattgccaaaaactatgaaaccccggacgctgtagaagcgagccagaaaggcagtaacgatttcgtgaccaacgtagat                                  >  2:32746‑M1/1‑36 (MQ=255)                                                                                                                       aTCCGAGGCTGA‑ACATCGCCGTgcgcgcagcgcgcagcgcgcaaggcgggtaatttaattgccaaaaactatgaaaccccggacgctgtagaagcgagccagaaaggcagtaacgatttcgtgaccaacgtagataaagctgccgaagc                    <  2:53174‑M1/149‑128 (MQ=255)                                                                                                                          cGATGCTGA‑ACATCGCCGTgcgcgcagcgcgcagcgcgcaaggcgggtaatttaattgccaaaaactatgaaaccccggacgctgtagaagcgagccagaaaggcagtaacgatttcgtgaccaacgtagataaagctgccgaagcggt                 >  2:202348‑M1/1‑19 (MQ=255)                                                                                                                              gCTGA‑ACATCGCCGTgcgcgcagcgcgcagcgcgcaaggcgggtaatttaattgccaaaaactatgaaaccccggacgctgtagaagcgagccagaaaggcagtaacgatttcgtgaccaacgtagataaagctgccgaagcggtgatt             >  2:197622‑M1/1‑15 (MQ=255)                                                                                                                               cTGA‑ACATCGCCGTgcgcgcagcgcgcagcgcgcaaggcgggtaatttaattgccaaaaactatgaaaccccggacgctgtagaagcgagccagaaaggcagtaacgatttcgtgacccacgtcgatacagctgccgaagcggtgatta            >  2:30291‑M1/1‑14 (MQ=255)                                                                                                                               cTGA‑ACATCGCCGTgcgcgcagcgcgcagcgcgcaaggcgggtaatttaattgccaaaaactatgaaaccccggacgctgtagaagcgagccagaaaggcagtaacgaattcgtgaccaacgtagataaagctgccgagtggg                  >  1:241873‑M1/1‑14 (MQ=255)                                                                                                                                tGA‑ACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAAC‑GATTTCGTGACCAACGTAGATAAAGCTGCCGCAGCGGTGATTATc                >  1:50099/1‑143 (MQ=255)                                                                                                                                  a‑aCATCGCCGTgcgcgcagcgcgcagcgcgcaaggcgggtaatttaattgccaaaaactatgaaaccccggacgctgtagaagcgagtcagaaaggcagtaacgatttcgtggccaacgtagataaagctgccgaagcggtgattatcg         >  2:196584‑M1/1‑11 (MQ=255)                                                                                                                                         gCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAAC‑GATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGATTATCGACACGATTCGTaa  >  2:375613/1‑149 (MQ=255)                                                                                                                                           cGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACACCGGCCGACGTAGAAGCGAGCCAGAAAGGAAGCAACGGGCGGCCTG‑CCGACGTAAACAAATCGGCCGAAGCGGTGATTATCGACACGAtcc      >  1:374330/1‑141 (MQ=255)                                                                                                                                            gTgcgcgcagcgcgcagcgcgcaaggcgggtaatttaattgccaaaaactatgaaaccccggacgctgtagaagcgagccagaaaggcagtaacgatttcgtgaccaacgtagataaagctgccgaagcggtgattatcgaca      >  1:268160‑M1/1‑2 (MQ=255)                                                                                                                                             |                                                                                                                                                  TTTCTTCATCTAAAGATTATTCACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGA‑ACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAAC‑GATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGATTATCGACACGATTCGTAA  >  CP009273/2656687‑2656970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.