| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 444,779 | C→T | 100% | W440* (TGG→TAG) | cyoB ← | cytochrome o ubiquinol oxidase subunit I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 444,779 | 0 | C | T | 86.8% | 28.9 / ‑0.6 | 17 | W440* (TGG→TAG) | cyoB | cytochrome o ubiquinol oxidase subunit I |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (5/9); total (8/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.68e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Not supported by required number of reads on both strands. | |||||||||||
CATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTG‑CGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCC > CP009273/444667‑444915 | cATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGa < 1:297867/143‑1 (MQ=255) gCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCAcgccgc < 2:419625/149‑1 (MQ=255) acgccaCGAAGAAGCCGATGACCCAGAACCAGCGCGCGCGTTTACCCCAGGTTTCGTTCAGTGTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTg‑c < 2:77579/144‑1 (MQ=255) aGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGACCCCGTCATgg > 1:391312/1‑143 (MQ=255) gCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTAACAGCTTCTCAGCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTg‑cgc > 2:316838/1‑149 (MQ=255) agaagCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCGCGCCGCCGATGAGCACGTTATGGAAGTG‑CGCAATCAgg > 2:304099/1‑149 (MQ=255) ccGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTG‑CGCAATCAg < 1:186498/143‑1 (MQ=255) gatgatCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTACAGTTTGAAACCGAACGCTTTAGGCAACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTG‑CGCAATCAGGAACAGGc > 2:78043/1‑149 (MQ=255) atCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTG‑CGCAATCAGGAACAGGCTGtt > 2:73797/1‑149 (MQ=255) cagaacGCGCGTTTACCCCAGGTTTTGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTG‑CGCAATCAGGAACAGGCTGTTATGCAGAAc < 2:267435/149‑1 (MQ=255) tACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTG‑CGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTcc < 1:170139/143‑1 (MQ=255) ccccAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTG‑CGCAATCAGGCACAGGCTGTTATGCAGAACGACGTCcgc > 1:156034/1‑143 (MQ=255) tttCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTG‑CGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGt < 1:261212/143‑1 (MQ=255) cGTTCAGTTTGACACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGCG‑CGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACg < 1:291352/143‑1 (MQ=255) cGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTG‑GGCAATCAGGAACAGGCTGTTATGCAGAACGCAGTCCGCGCCCGGTACGGCcagc > 2:333845/1‑149 (MQ=255) ttCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTCT‑GCAGTGACTTAGTCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcagcag > 2:426869/1‑149 (MQ=255) aaCGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTG‑CGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATccc < 2:163291/149‑1 (MQ=255) | CATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTG‑CGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCC > CP009273/444667‑444915 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |