| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,217,123 | A→G | 19.0% | Y487C (TAC→TGC) | dld → | D‑lactate dehydrogenase, FAD‑binding, NADH independent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,217,123 | 0 | A | G | 19.0% | 54.2 / 7.8 | 31 | Y487C (TAC→TGC) | dld | D‑lactate dehydrogenase, FAD‑binding, NADH independent |
| Reads supporting (aligned to +/- strand): ref base A (13/12); new base G (2/4); total (15/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01 | |||||||||||
ATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATA > CP009273/2216987‑2217269 | aTTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGT‑GCACAAGCTCTATTACGGCCATTTTAt > 1:313233/1‑149 (MQ=255) tCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGT‑GCACAAGCTCTATTGCGGCCATTTTAtgt < 1:316204/149‑1 (MQ=255) gTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGc > 1:170925/1‑149 (MQ=255) tCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTCCCAGATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGCTATg < 1:318201/149‑1 (MQ=255) cGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTc > 1:25415/1‑149 (MQ=255) aTTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAgg > 1:29405/1‑149 (MQ=255) cTGGCGTTGGATATCGCTCTGCGGCGTAACGCCACCTAGTGGGATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGTTTACATATTGAAAAAAGGCGt < 1:330972/149‑1 (MQ=255) cTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGt < 2:313233/149‑1 (MQ=255) tgtaaatagCTCGGCAGCGCAACGACACCGAGTGGTAAGAGCATTTGCGGGGGGAGAGCGACAGCGAGCTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGAAAAAAAAAAAAAAAAAGGCGTGGAtgtg < 2:112073/141‑1 (MQ=255) tatCGCTCTGCGGCGTAACGACACCGAGTGGTAGGCGCATTTACCGCCGGAGATCGACAGCCAGCTGGT‑GCACAAGCTCTATTGCGGCCATTTTATGTGCGATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATg < 1:19069/149‑1 (MQ=255) catCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGT‑GCACAAGCTCTATTGCGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATg < 1:56332/148‑1 (MQ=255) tctGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGT‑GCACAAGCTCTATTGCGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTaa > 1:154906/1‑149 (MQ=255) gcggcgTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGGTTACATAGTGAACAAAGACGTGGATGTGCATGCGTTCAAAg > 2:168471/1‑149 (MQ=255) cggcgTAACGCCACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACA‑CAACTTTGTGGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGa < 1:439609/149‑1 (MQ=255) ggcgTAACGACACCGAGTTGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGaa < 2:393809/149‑1 (MQ=255) gaagacgggTGGTATGAGCATTTACAGCCGGAGGACGACAGCCAGCTGGT‑GCACAAGCTCGATTGCGGCCCTCTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAGAGGGCGAGGATGGACATGCGGTAAAAGAACAGAtagt > 2:328626/9‑146 (MQ=255) gTATGACCATTTACCGCCGGAGATCGACAGCCAGCTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTAc < 1:203532/149‑1 (MQ=255) tATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGT‑GCACAAGCTCTATTACGGCCATTTTAAGTGCTATGTCTTCCATCAGGCTTACCTAGTGAAAGAACGCGCGGATGCGCATGCGCTGGAAGAGCAGATGCGGGAAGTGCTAca > 2:322939/1‑149 (MQ=255) aCCGCCGGAGATCGACAGCCAGCTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAgcgcggcg < 1:168471/149‑1 (MQ=255) ccgGAGATCGACAGCCAGCTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCa < 2:29405/149‑1 (MQ=255) aTCGACAGCCAGCTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTAccc > 1:400780/1‑149 (MQ=255) tCGACAGCCAGCTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCt > 1:401758/1‑149 (MQ=255) gACAGCCAGCTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGc > 1:353897/1‑149 (MQ=255) ccagcTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGc > 1:38916/1‑149 (MQ=255) agagcTTGT‑GCACCAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGCACAGCTACATGAACTGCGACAGCAGCGCGGCGCGCAGTACCCTGCCGAGc > 1:87587/3‑149 (MQ=255) cagcTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCa > 2:57962/1‑149 (MQ=255) cTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATaa > 1:388447/1‑149 (MQ=255) gCACAAGCTCTATTACGGCCATTTTAGGTGCTATGTCTTCCATCAGGATTACAAAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCg < 2:434799/149‑1 (MQ=255) ctctATTGCGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCAttt < 2:154906/149‑1 (MQ=255) ctctATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCAttt < 1:72764/149‑1 (MQ=255) ctACGGACATTTTATGTGCTATGTATTACATCATGACAACAAAGAGAAAAAAGGGGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGATCATAACGTCGGTCATTTGtata < 2:159805/148‑1 (MQ=255) | ATTCGTTATCAGGCGGTGCATTCCGATGAAGTCGAAGACATTCTGGCGTTGGATATCGCTCTGCGGCGTAACGACACCGAGTGGTATGAGCATTTACCGCCGGAGATCGACAGCCAGCTGGT‑GCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATA > CP009273/2216987‑2217269 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |